🦴 A picture is worth a thousand words—but this one speaks of #bone formation, remodelling, repair, and renewal!

The small white/grey dots are #osteocytes

🔬 Scanning electron microscopy after resin cast etching
🔍 rdcu.be/ebD4R

#biomaterials #biomineralization #microscopy #electronmicroscopy 🧪

#Grogu spotted in #bone!

🦴🔬🧪🩻

#yoda #babyyoda #starwars #science #sciencefiction #imaging #microscopy #electronmicroscopy #biomineralization #osteocytes

🦴🔬🩻🧪🥼 Last #imaging session for 2024. Looking at some #bone using an environmental scanning electron microscope! See you again in 2025!

#microscopy #bioimaging #electronmicroscopy #biomineralization #collagen #apatite #osteocytes

2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS) - Current Osteoporosis... Purpose of Review Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2...

Interested in osteocyte imaging?
In the summer, we published a review where we discuss common approaches and pitfalls for 2D and 3D imaging:

#bone #osteocytes #imaging
@ects.bsky.social (Hoping this is the correct ECTS)

doi.org/10.1007/s119...

This is a scanning electron microscopy image of osteocytes clinging to the surface of a titanium implant that has been surface modified by selective laser ablation to create a hierarchical, macro-micro-nano topography: https://pubs.acs.org/doi/full/10.1021/ab500127y The imaging technique is called Resin Cast Etching: https://link.springer.com/article/10.1007/s00223-022-01058-9

🦴🔬🧪 #Osteocytes in #bone around an osseointegrated titanium #implant

Osteocytes are mechanosensory cells that form a neuron-like network within bone and can regulate bone remodelling.

pubs.acs.org/doi/full/10....

#biomineralization #biology #orthopaedics #science #electronmicroscopy #imaging

The proximity of osteocytes (Ot) to implant surfaces observed with (a) histology and (b) BSE-SEM. (c) Resin cast etching allows visualization of osteocyte attachment to implant surfaces. (d) Osteocyte attachment to an acid-etched surface. (e) Interdigitation of canaliculi with the implant surface (arrowheads). (f, g) Canaliculi (Ot.Ca) adherent to the surface of a laser-ablated implant. (h) Cross-sectional view of canaliculi (*) in close association with surface TiO2 layer of a laser-ablated implant (HAADF-STEM). (i) Osteocytes align parallel to the lamellar direction, which closely follows the microcontour of the laser-ablated implant surface. (j) Adjacent to the implant surface, osteocytes form an interconnected network. (k) Canaliculi (arrowheads) can be identified within the first several micrometers from the implant surface (HAADF-STEM). (l) Mineralized collagen fibrils appear to “wrap around” the canaliculus (*).

🧪🔬🦴💠 A Review of the Impact of Implant Biomaterials on Osteocytes

journals.sagepub.com/doi/full/10....

#bone #biomaterials #osteocytes #microscopy #histology #electronmicroscopy #imaging #biology #science #aging #biomechanics #osteoporosis #bonehealth #pathology

Mine was very broadly on osseointegration of metal implants in #bone, with focus on bone cells called #osteocytes.

gupea.ub.gu.se/bitstream/ha...

Regrettably, I have never given an entire lecture on this to undergraduates.

Many growth factors affecting bone formation and resorption are released from the extracellular matrix and cells (such as osteoblasts) in such bone fragments, including transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP), fibroblast growth factor (FGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa-β ligand (RANKL), and osteoprotegerin (OPG). Here, scanning electron microscopy shows autogenous bone fragments surrounded by new bone, one week after a surgical procedure involving placement of titanium implants in rat tibia. Read more: https://link.springer.com/article/10.1007/s00223-017-0283-2

🧪🔬🦴Small fragments of #bone generated during #surgery can have a strong osteoconductive effect. Bone #drilling can produce fragments of various sizes that provide a suitable substrate and present a large surface area for new bone formation. The presence of #osteocytes confirms such #osteoconduction.

🦴🔬#Osteocyte – the master orchestrator of #bone #remodeling. ~42 billion in the average adult human skeleton, #osteocytes make up ~90–95% of all #cells within bone, interconnected by ~3.7 trillion dendritic projections with an estimated, total end-to-end length of 175,000 km 🔍