Swapped and non-swapped TRAAK states co-exist in membranes at a ratio influenced by temperature - Nature Communications TRAAK is expressed in the nervous system and helps regulate neuronal excitability. Here, the authors use EPR to quantify the relative populations of TRAAK’s swapped and non-swapped cap states in membr...

In our study in @natcomms.nature.com we used #EPR #DEER #PELDOR spectroscopy with single subunit heterologous spin labelling and monitored the entire cap conformational ensemble of the human mechanosensitive TRAAK channel and showed it adopts two states in membranes.

www.nature.com/articles/s41...

We also used #EPR #PELDOR studies and #MDsimulations to propose how protonation affects the conformational ensemble and induces coformational changes allowing the movement of antimicrobial peptides and protons across the #membrane.