Expanding the landscape of BREX diversity: uncovering multi-layered functional frameworks and identification of novel BREX-related defense systems Abstract. Despite extensive scrutiny of BREX systems, several overarching questions persist regarding the functional modalities of individual components an

Happy to share our article On BREX @narjournal.bsky.social We here redefine the core of BREX, uncover hidden functional domains, and identify 3 new BREX-related(BR) RM-like defense systems, expanding the BREX landscape. @rakesh-raj21.bsky.social #Phagedefense #BREX #prokaryoticimmunity

Where do these dinucleotides come from?
We propose that during phage infection, phage-encoded nucleases chop up the host chromosome, generating pdNG fragments as byproducts.
ApeA essentially detects its own genome being destroyed 🔪🧬 (7/11)
#Microbiology #PhageDefense

OLD amputates the anticodon arm of tRNAs during P2–Lambda interference Abstract. ATPase-coupled Toprim (Topoisomerase-primase) nucleases, known as Overcoming Lysogenization Defect (OLD) proteins, are crucial for diverse antiph

Using a phage-inspired genetic screen and biochem, NEB, Harvard Medical School, and University of Illinois Urbana-Champaign researchers discovered OLD inactivates tRNAs by a completely novel mechanism: severing anticodon arms! https://nebiolabs.com/4rysTEk
#tRNA #PhageDefense

📝 Online now - the Review "Proteases in bacteriophage defense systems and their potential in bioengineering" from Stan Brouns and colleagues.

#PhageDefense #ProteaseAssociatedDefenseSystems #PADS #ProteinEngineering

Read it here: authors.elsevier.com/sd/article/S...