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#TiH
Somatic embryogenesis in banana (Musa spp.): A review of methodologies and prospects for enhanced propagation.
Details: maxapress.com/article/doi/10.48130/tih...
#PlantBiotechnology #Agriculture

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Lara

Lara

Our new group member Lara focuses on cloning arGSTs from plants! ๐Ÿงฌโœจ Thanks to Najnin for excellent support in the lab.

www.izmb.uni-bonn.de/en/pbb/team

#PlantSciences #Puckerlab #UniBonn #PlantBiotechnology #MolecularBiology #Anthocyanins #Evolution

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In this study, Cao et al. explored whether the cut-dip-budding strategy could be adapted for soybean to achieve efficient, tissue culture-free genetic transformation.
doi.org/10.1016/j.xi...
#MolecularBreeding #plantbiotechnology

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Grafting trick could let us gene-edit a huge variety of plants Many plants including cocoa, coffee and avocado cannot be gene-edited but a technique involving grafting could change that, opening the door to more productive and nutritious varieties

Grafting trick could let us gene-edit a huge variety of plants #Science #Biology #Genetics #GeneEditing #PlantBiotechnology #AgricultureInnovation
purescience.news/article?id=9...

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New pathway engineered into plants lets them suck up more COโ‚‚ Lots of people are excited about the idea of using plants to help us draw down some of the excess carbon dioxide we've been pumping into the atmosphere. It would be nice to think that we could reforest...

New pathway engineered into plants lets them suck up more COโ‚‚ #Science #Biology #Ecology #ClimateChange #PlantBiotechnology #CarbonCapture

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New pathway engineered into plants lets them suck up more COโ‚‚ Engineered pathway lets carbon be plugged directly into key metabolic pathways.

New pathway engineered into plants lets them suck up more COโ‚‚ #Science #Biology #Ecology #ClimateChange #PlantBiotechnology #CarbonCapture

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๐Ÿš€ Excited to share our new publication in Lab on a Chip!

Our article โ€œParallel DLD Microfluidics for Chloroplast Isolation and Sortingโ€ has just been accepted ๐Ÿ‘‰ lnkd.in/d3Z93fRA

#Microfluidics #LabOnAChip #PlantBiotechnology #Chloroplasts #Innovation

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Wheat Plants Engineered To Make Their Own Fertilizer All the science news you can handle in a single feed

Wheat Plants Engineered To Make Their Own Fertilizer #Science #Biology #Botany #SustainableAgriculture #PlantBiotechnology #GeneticEngineering

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Figure 1. The tartronyl-coenzyme A (TaCo) pathway as a photorespiratory bypass in plant chloroplast. 
(a) A schematic representation of plant photorespiration pathway (black) and GS2/Fd-GOGAT Cycle (light blue) and the TaCo bypass (orange).

Figure S1. Schematic diagram depicting the multigene expression vectors TaCo-pYL1300H designed for TaCo bypass implementation.

Each enzyme was fused at the N-terminus with RC2, a highly efficient chloroplast-targeting peptide, and at the C-terminus with specific tags: 3 ร— FLAG for glycolyl-CoA synthase (GCS), 3 ร— HA for glycolyl-CoA carboxylase (GCCM5), and Myc for tartronyl-CoA reductase (TCR). 
Each cassette was driven by different promoters and terminators: the enhanced CaMV 35S promoter (proE35S) and OCS terminator for RC2-GCS-3ร—FLAG, the rice Actin promoter (proACT) and pea (Pisum sativum) 3A terminator for RC2-GCC-3ร—HA, and the maize (Zea mays) Ubiquitin promoter (proUbi) and NOS terminator for RC2-TCR-Myc.

Figure 1. The tartronyl-coenzyme A (TaCo) pathway as a photorespiratory bypass in plant chloroplast. (a) A schematic representation of plant photorespiration pathway (black) and GS2/Fd-GOGAT Cycle (light blue) and the TaCo bypass (orange). Figure S1. Schematic diagram depicting the multigene expression vectors TaCo-pYL1300H designed for TaCo bypass implementation. Each enzyme was fused at the N-terminus with RC2, a highly efficient chloroplast-targeting peptide, and at the C-terminus with specific tags: 3 ร— FLAG for glycolyl-CoA synthase (GCS), 3 ร— HA for glycolyl-CoA carboxylase (GCCM5), and Myc for tartronyl-CoA reductase (TCR). Each cassette was driven by different promoters and terminators: the enhanced CaMV 35S promoter (proE35S) and OCS terminator for RC2-GCS-3ร—FLAG, the rice Actin promoter (proACT) and pea (Pisum sativum) 3A terminator for RC2-GCC-3ร—HA, and the maize (Zea mays) Ubiquitin promoter (proUbi) and NOS terminator for RC2-TCR-Myc.

Figure 1. The tartronyl-coenzyme A (TaCo) pathway as a photorespiratory bypass in rice chloroplast.

(j) Biomass per plant (n = 6).
(k) Grain yield per plant (n = 6).
(l) Grain number per panicle (n = 6).
(m) Mature paddy rice grains. Scale bar, 5 mm.
(n) Grain length (n = 20).
(o) Grain width (n = 20).
(p) The 1000-grain weight (n = 6).

Bars (j) to (k) and (n) to (p) represent means ยฑ SDs. Significant differences (P < 0.05) are indicated by letters using ANOVA with Tukey's multiple-comparison test (Data S4).

Figure 1. The tartronyl-coenzyme A (TaCo) pathway as a photorespiratory bypass in rice chloroplast. (j) Biomass per plant (n = 6). (k) Grain yield per plant (n = 6). (l) Grain number per panicle (n = 6). (m) Mature paddy rice grains. Scale bar, 5 mm. (n) Grain length (n = 20). (o) Grain width (n = 20). (p) The 1000-grain weight (n = 6). Bars (j) to (k) and (n) to (p) represent means ยฑ SDs. Significant differences (P < 0.05) are indicated by letters using ANOVA with Tukey's multiple-comparison test (Data S4).

Great work by Chen et al. (2025) on introducing the tartronyl-CoA (TaCo) pathway into rice chloroplasts as a synthetic, carbon-positive photorespiratory bypass to enhance CO2 fixation efficiency and increase biomass, grain yield and grain size in rice ๐ŸŒพ. #PlantBiotechnology
๐Ÿ”— doi.org/10.1111/pbi....

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Greenpeace warnt vor grรผner Gentechnik โ€“ zu Recht? | SWR Wissen
Greenpeace warnt vor grรผner Gentechnik โ€“ zu Recht? | SWR Wissen YouTube video by SWR Wissen

This is a fantastic documentary on #PlantBiotechnology, genetically engineered #PlantGMOs, & the anti-scientific disinformation campaigns that #Greenpeace has been running against them.
In German, but there are English subtitles.

SWR is German public broadcaster, & I don't see a COI.

#PlantScience

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LR students leave their mark at 2025 North Carolina Academy of Science meeting Lenoir-Rhyne University students showcased their academic excellence and research expertise at the 2025 North Carolina Academy of Science (NCAS) annual meeting in late March.

I'm very proud of our #undergraduateresearch students. My advisees Ivy & Elijah presented their #cotton projects in #plantbiotechnology, #plantbiology at #NCAS2025. ๐Ÿงช๐Ÿ”ฌ๐ŸŽ‰๐ŸŽ‰

www.lr.edu/news/lr-stud...

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Advances in Subcellular Accumulation Design for Recombinant Protein Production in Tobacco | BioDesign Research Plants and their use as bioreactors for the generation of recombinant proteins have become one of the hottest topics in the field of Plant Biotechnology and Plant Synthetic Biology. Plant bioreactors ...

Tobacco bioreactors boost recombinant protein yield via subcellular strategies! #PlantBiotechnology #SyntheticBio
Details: spj.science.org/doi/10.34133/bdr.0047

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Diagram illustrating the 4 stages in DNA-free base editing: (1) in vitro transcription, (2) protoplast transfection, (3) protoplast regeneration, and (4) base-edited plants.

Diagram illustrating the 4 stages in DNA-free base editing: (1) in vitro transcription, (2) protoplast transfection, (3) protoplast regeneration, and (4) base-edited plants.

This remarkable new RNA-based adenine and cytosine #BaseEditing system achieves targeted and efficient A-to-G and C-to-T conversions in lettuce, with potential applications in #crop breeding and #PlantBiotechnology.
doi.org/10.1111/jipb...
#PlantScience

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We're excited to ๐—ผ๐—ณ๐—ณ๐—ถ๐—ฐ๐—ถ๐—ฎ๐—น๐—น๐˜† ๐—ผ๐—ฝ๐—ฒ๐—ป ๐—ฟ๐—ฒ๐—ด๐—ถ๐˜€๐˜๐—ฟ๐—ฎ๐˜๐—ถ๐—ผ๐—ป ๐—ฎ๐—ป๐—ฑ ๐—ฎ๐—ฏ๐˜€๐˜๐—ฟ๐—ฎ๐—ฐ๐˜ ๐˜€๐˜‚๐—ฏ๐—บ๐—ถ๐˜€๐˜€๐—ถ๐—ผ๐—ป๐˜€ ๐—ณ๐—ผ๐—ฟ ๐˜๐—ต๐—ฒ ๐Ÿต๐˜๐—ต ๐—”๐—ป๐—ป๐˜‚๐—ฎ๐—น ๐—จ๐—™ ๐—ฃ๐—น๐—ฎ๐—ป๐˜ ๐—ฆ๐—ฐ๐—ถ๐—ฒ๐—ป๐—ฐ๐—ฒ ๐—ฆ๐˜†๐—บ๐—ฝ๐—ผ๐˜€๐—ถ๐˜‚๐—บ! Check out our website for more information: tinyurl.com/4kz8hk3b

#PlantScience #PlantPathology #Agronomy #Symposium #UniversityofFlorida #Plantbiotechnology #Plantphysiology

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CRISPRi-based circuits to control gene expression in plants - Nature Biotechnology Programmable and reversible CRISPRi-based genetic circuits function in a variety of plants.

๐Ÿ“œ CRISPRi-based circuits to control gene expression in plants

๐Ÿง‘โ€๐Ÿ”ฌ Muhammad Adil Khan, Brendan N. Kidd, Ryan Lister, et al.

๐Ÿ“” @natureportfolio.bsky.social Biotechnology

๐Ÿ”— www.nature.com/articles/s41...

๐Ÿ”Š www.nature.com/articles/s41...

#๏ธโƒฃ #PlantScience #PlantSynthBio #PlantBiotechnology #CRISPR

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A quote from Marc van Montagu, stating that "Nonetheless, this was a scientific race conducted on amicable terms, with information being exchanged and synchronized publication of many of the notable papers.".

A quote from Marc van Montagu, stating that "Nonetheless, this was a scientific race conducted on amicable terms, with information being exchanged and synchronized publication of many of the notable papers.".

The development of plant transformation revolutionized #PlantSciences, was vital to kickstart the field of #PlantMolecularBiology, and launched the #PlantBiotechnology / PlantGMOs sector. And, remarkably, the race was competitive but fair, with high-profile papers for all involved!

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