His group was first to report stable #PlantCRISPR plants in 2014 @theplantjournal.bsky.social :
onlinelibrary.wiley.com/doi/10.1111/...

Here's a recent review of him et al. on plant genome editing:
bsky.app/profile/soms...

And here, one on chromosome engineering:
bsky.app/profile/soms...

Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level - Nature Communications Genetic variance is vital for breeding programs and mutant screening, yet traditional mutagenesis methods wrestle with genetic redundancy and a lack of specificity in gene targeting. Here the authors ...

📜 Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level

🧑‍🔬 Amichai Berman, Ning Su, Yuqin Zhang, @eilonshani.bsky.social, et al.

📔 @natcomms.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantTools #Tomato #PlantCRISPR

A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha The liverwort Marchantia polymorpha is a widely used model organism for studying land plant biology, which has also proven to be a promising testbed for bioengineering. CRISPR/Cas9 technology has emer...

📜 A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha

🧑‍🔬 Eftychios Frangedakis, @jmhibberd.bsky.social, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantGenomics #PlantMethods #PlantEvoDevo #Marchantia #PlantCRISPR

The future of genome editing in plants - Nature Plants Future genome editing in plants will mimic the natural evolutionary processes that shape genomes. It will be used to reshape plant genomes in a manner that could have happened naturally, but more prec...

📜 The future of genome editing in plants

🧑‍🔬 Larry Gilbertson, Holger Puchta, R. Keith Slotkin

📔 @natplants.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCRISPR #PlantGE #PlantGenomics #PlantNGTs #GenomeEditing #PlantGenomeEditing

A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants An all-in-one CRISPR toolbox is developed to aid guide RNA library-based loss-of-function and gain-of-function screens in plants.

📜 A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants

🧑‍🔬 Yanhao Cheng, Yiping Qi, et al.

📔 @theplantcell.bsky.social

🔗 academic.oup.com/plcell/advan...

#️⃣ #PlantScience #PlantCRISPR #PlantMethods #PlantGenomics #PlantNGTs #PlantGE #PlantGenomeEditing

Intronization of Coding Sequences for Optimization of Gene Expression The amount of proteins present in cells of living organisms depends on various factors, including the rate of transcription of coding sequences, the efficiency with which pre-mRNA transcripts are proc...

📜 Intronization of Coding Sequences for Optimization of Gene Expression

🧑‍🔬 @cbtomsen.bsky.social, Sylvestre Marillonnet

📔 CRISPR-Cas Methods

🔗 link.springer.com/10.1007/978-...

#️⃣ #PlantScience #PlantCRISPR #PlantNGTs #PlantGenomeEditing #Translation #PlantGenomics #PlantGenetics

Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis We introduced two targeted DNA double-strand breaks on the same Arabidopsis chromosome using CRISPR-Cas9 and replaced the FLOWERING LOCUS T ( FT ) promoter with that of a histone variant gene through ...

📜 Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis

🧑‍🔬 Takashi Nobusawa, Makoto Kusaba, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantNGTs #PlantGenomeEditing #PlantGenomics #PlantCRISPR

Beyond a few bases: methods for large DNA insertion and gene targeting in plants Large, precise DNA insertions remain challenging to achieve in plants due to both biological and technical bottlenecks. This review highlights recent advances in CRISPR-Cas systems, recombinases, and...

📜 Beyond a few bases: methods for large DNA insertion and gene targeting in plants

🧑‍🔬 Katie Vollen, Jose M. Alonso, Anna N. Stepanova

📔 @theplantjournal.bsky.social

🔗 onlinelibrary.wiley.com/doi/10.1111/...

#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #GenomeEditing #PlantGenomeEditing

CRISPR–Cas applications in agriculture and plant research - Nature Reviews Molecular Cell Biology CRISPR–Cas-mediated genome, epigenome and transcriptome engineering in crops is crucial as the human population grows and climate deteriorates. This Review discusses new CRISPR–Cas tools for precise e...

📜 CRISPR–Cas applications in agriculture and plant research

🧑‍🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.

📔 @natrevmcb.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing

Advancing programmable gene expression in plants using CRISPRi-based Boolean gates - Nature Biotechnology To advance the toolset for controlling plant gene expression, we developed a CRISPR interference-based platform for the construction of synthetic Boolean logic gates that is functional in multiple pla...

Advancing programmable gene expression in plants using CRISPRi-based Boolean gates

Adil Khan & Ryan Lister on their work published in @natureportfolio.bsky.social Biotechnology

#PlantScience #PlantSynBio #PlantCRISPR #PlantNGTs #PlantGenomics

www.nature.com/articles/s41...