Two computer screens. The right-hand screen shows Holger Puchta infront of a screen presenting his work. The left-hand screen shows his presentation. The Nobel Price medal is shown, portraits of Emmanuelle Charpentier and Jennifer Doudna, as well as a sketch showing the Cas9 enzyme cutting DNA. The slide is titled "A tsunami came over biology in 2012".

We had Holger Puchta from @kit.edu here today.
He gave a fantastic and very entertaining #PlantScience talk about his pioneering journey in #PlantGenomeEditing, from the 1990s until today.
His new chromosome engineering breakthroughs are 🤯🤯🤯.

Have a look at the papers I'll link below, too.

The future of genome editing in plants - Nature Plants Future genome editing in plants will mimic the natural evolutionary processes that shape genomes. It will be used to reshape plant genomes in a manner that could have happened naturally, but more prec...

📜 The future of genome editing in plants

🧑‍🔬 Larry Gilbertson, Holger Puchta, R. Keith Slotkin

📔 @natplants.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCRISPR #PlantGE #PlantGenomics #PlantNGTs #GenomeEditing #PlantGenomeEditing

A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants An all-in-one CRISPR toolbox is developed to aid guide RNA library-based loss-of-function and gain-of-function screens in plants.

📜 A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants

🧑‍🔬 Yanhao Cheng, Yiping Qi, et al.

📔 @theplantcell.bsky.social

🔗 academic.oup.com/plcell/advan...

#️⃣ #PlantScience #PlantCRISPR #PlantMethods #PlantGenomics #PlantNGTs #PlantGE #PlantGenomeEditing

Intronization of Coding Sequences for Optimization of Gene Expression The amount of proteins present in cells of living organisms depends on various factors, including the rate of transcription of coding sequences, the efficiency with which pre-mRNA transcripts are proc...

📜 Intronization of Coding Sequences for Optimization of Gene Expression

🧑‍🔬 @cbtomsen.bsky.social, Sylvestre Marillonnet

📔 CRISPR-Cas Methods

🔗 link.springer.com/10.1007/978-...

#️⃣ #PlantScience #PlantCRISPR #PlantNGTs #PlantGenomeEditing #Translation #PlantGenomics #PlantGenetics

Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis We introduced two targeted DNA double-strand breaks on the same Arabidopsis chromosome using CRISPR-Cas9 and replaced the FLOWERING LOCUS T ( FT ) promoter with that of a histone variant gene through ...

📜 Promoter replacement by genome editing creates gain-of-function traits in Arabidopsis

🧑‍🔬 Takashi Nobusawa, Makoto Kusaba, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantNGTs #PlantGenomeEditing #PlantGenomics #PlantCRISPR

Beyond a few bases: methods for large DNA insertion and gene targeting in plants Large, precise DNA insertions remain challenging to achieve in plants due to both biological and technical bottlenecks. This review highlights recent advances in CRISPR-Cas systems, recombinases, and...

📜 Beyond a few bases: methods for large DNA insertion and gene targeting in plants

🧑‍🔬 Katie Vollen, Jose M. Alonso, Anna N. Stepanova

📔 @theplantjournal.bsky.social

🔗 onlinelibrary.wiley.com/doi/10.1111/...

#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #GenomeEditing #PlantGenomeEditing

CRISPR–Cas applications in agriculture and plant research - Nature Reviews Molecular Cell Biology CRISPR–Cas-mediated genome, epigenome and transcriptome engineering in crops is crucial as the human population grows and climate deteriorates. This Review discusses new CRISPR–Cas tools for precise e...

📜 CRISPR–Cas applications in agriculture and plant research

🧑‍🔬 Aytug Tuncel, Changtian Pan, Yiping Qi, et al.

📔 @natrevmcb.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantNGTs #PlantCRISPR #Agriculture #PlantGenomics #PlantGenomeEditing

China adds gene-edited wheat to growing biotech crop list | AGDAILY For the first time, China has granted safety approval to gene-edited wheat, signaling a cautious step forward in Beijing's adoption of GM food crops.

"China adds gene-edited wheat to growing biotech crop list"

via AGDaily, by Heidi Crnkovic.

“China has approved the safety of gene-edited wheat for the first time"

www.agdaily.com/crops/china-...

#PlantScience #PlantGMOs #Agriculture #Wheat #PlantGenomeEditing

CRISPR gene editing to improve crop resistance to parasitic plants Parasitic plants pose a significant threat to global agriculture, causing substantial crop losses and hampering food security. In recent years, CRISPR (Clustered Regularly Interspaced Short Palindromi...

📜 CRISPR gene editing to improve crop resistance to parasitic plants

🧑‍🔬 Min-Yao Jhu, Evan E. Ellison, Neelima R. Sinha

📔 Frontiers in Genome Editing

🔗 www.frontiersin.org/articles/10....

#️⃣ (Feeds) #PlantScience #ParasiticPlants #PlantGenomeEditing #PlantImmunity