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#RealTimeMicroscopy
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Finally got the SIM perfectly calibrated on all four channels ๐ŸŒˆ (movie shows a 15x15 ฮผm area inside a cell) #realtimemicroscopy

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Mmmh no wonder my mosaic stitching is off there's an inversion in the Y stage coordinate in the metadata #realtimemicroscopy

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Session on ye olde STORM scope to teach @rlouis.bsky.social how we did it in the good old days #realtimemicroscopy

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Real-time, High-throughput Super-resolution Microscopy via Panoramic Integration
Bozdag, G. O., Burnetti, A. J. et al.
Paper
Details
#RealTimeMicroscopy #SuperResolutionImaging #PanoramicIntegrationTech

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Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin ๐Ÿ”ฅ๐Ÿ•ถ๏ธ #realtimemicroscopy

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Someone's got to take these STORM images while @roylab-ucsd.bsky.social enjoys fancy hotels #realtimemicroscopy

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AX-NSPARC confocal demo delivers so far. Large field of view *and* super-resolution! Cell phone capture straight from the scope monitor #realtimemicroscopy

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Actin and clathrin-coated pits are reassuringly found along the plasma membrane on the 3D-SIM stacks #realtimemicroscopy

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Straight out if the N-SIM #realtimemicroscopy

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In my happy place #realtimemicroscopy

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Neuron after a day in culture stained for actin (gray), microtubules (orange) and DNA (blue). It looks like a psychedelic drunk starfish

Neuron after a day in culture stained for actin (gray), microtubules (orange) and DNA (blue). It looks like a psychedelic drunk starfish

Showing the SIM ropes to our new facility boss @bertrandvernay.bsky.social with nice baby neurons prepared by Florence! #realtimemicroscopy

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Trying some #FRAP with keratinocytes and CellMask on a Stellaris5.
Sample by Nuun Wisetyaningsih
#realtimemicroscopy ๐Ÿ”ฌ

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Look at that absolute unit of a glia (pic is 170x160 ยตm, 4900x5200 pixels). Good sample to test my new mosaic SIM workflow! #realtimemicroscopy

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Look at that absolute unit of a glia (pic is 170x160 ยตm, 4900x5200 pixels). Good sample to test my new mosaic SIM workflow! #realtimemicroscopy

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A fun nerdy day making SIM images and coding a mosaic stitching script to follow axons at 120 nm resolution #realtimemicroscopy

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Blinky blinky (finally movies on Bluesky!) #realtimemicroscopy #PIonthescope

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Blinky blinky #realtimemicroscopy #PIonthescope

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My kind of PSF #realtimemicroscopy

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Good ol' STORM of spectrin rings, it's been a while! #realtimemicroscopy

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Good ol' STORM of spectrin rings, it's been a while! #realtimemicroscopy

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in my happy place #realtimemicroscopy

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Dive into the tiny universe #realtimemicroscopy

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Even checking the TIRF-SIM with beads feels like fun after weeks without touching a scope #realtimemicroscopy

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Old school to new school โšก๐Ÿ”ฌ #realtimemicroscopy @terrymosoly

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This is fine #realtimemicroscopy #cellfie

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Freezing weather in Marseille means challenging room temperature for SIM calibration... Scope likes its toasty 23ยฐC. Looks like it's OK now #realtimemicroscopy

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Are SMLM calibrations better in a Sunday empty lab? Time to find out #realtimemicroscopy

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Taking care of a jealous SIM scope after three wonderful days at #SMLMS2022 #realtimemicroscopy

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Thanks to Yoshi and @christmachu the TIRF-SIM is now on fleek #realtimemicroscopy

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Nikon X Tokai X Ludin X Warner homemade collab is ready to play #realtimemicroscopy

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