Finally got the SIM perfectly calibrated on all four channels ๐ (movie shows a 15x15 ฮผm area inside a cell) #realtimemicroscopy
Mmmh no wonder my mosaic stitching is off there's an inversion in the Y stage coordinate in the metadata #realtimemicroscopy
Session on ye olde STORM scope to teach @rlouis.bsky.social how we did it in the good old days #realtimemicroscopy
Real-time, High-throughput Super-resolution Microscopy via Panoramic Integration
Bozdag, G. O., Burnetti, A. J. et al.
Paper
Details
#RealTimeMicroscopy #SuperResolutionImaging #PanoramicIntegrationTech
Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin ๐ฅ๐ถ๏ธ #realtimemicroscopy
Someone's got to take these STORM images while @roylab-ucsd.bsky.social enjoys fancy hotels #realtimemicroscopy
AX-NSPARC confocal demo delivers so far. Large field of view *and* super-resolution! Cell phone capture straight from the scope monitor #realtimemicroscopy
Actin and clathrin-coated pits are reassuringly found along the plasma membrane on the 3D-SIM stacks #realtimemicroscopy
Straight out if the N-SIM #realtimemicroscopy
In my happy place #realtimemicroscopy
Neuron after a day in culture stained for actin (gray), microtubules (orange) and DNA (blue). It looks like a psychedelic drunk starfish
Showing the SIM ropes to our new facility boss @bertrandvernay.bsky.social with nice baby neurons prepared by Florence! #realtimemicroscopy
Trying some #FRAP with keratinocytes and CellMask on a Stellaris5.
Sample by Nuun Wisetyaningsih
#realtimemicroscopy ๐ฌ
Look at that absolute unit of a glia (pic is 170x160 ยตm, 4900x5200 pixels). Good sample to test my new mosaic SIM workflow! #realtimemicroscopy
Look at that absolute unit of a glia (pic is 170x160 ยตm, 4900x5200 pixels). Good sample to test my new mosaic SIM workflow! #realtimemicroscopy
A fun nerdy day making SIM images and coding a mosaic stitching script to follow axons at 120 nm resolution #realtimemicroscopy
Blinky blinky (finally movies on Bluesky!) #realtimemicroscopy #PIonthescope
Blinky blinky #realtimemicroscopy #PIonthescope
My kind of PSF #realtimemicroscopy
Good ol' STORM of spectrin rings, it's been a while! #realtimemicroscopy
Good ol' STORM of spectrin rings, it's been a while! #realtimemicroscopy
in my happy place #realtimemicroscopy
Dive into the tiny universe #realtimemicroscopy
Even checking the TIRF-SIM with beads feels like fun after weeks without touching a scope #realtimemicroscopy
Old school to new school โก๐ฌ #realtimemicroscopy @terrymosoly
This is fine #realtimemicroscopy #cellfie
Freezing weather in Marseille means challenging room temperature for SIM calibration... Scope likes its toasty 23ยฐC. Looks like it's OK now #realtimemicroscopy
Are SMLM calibrations better in a Sunday empty lab? Time to find out #realtimemicroscopy
Taking care of a jealous SIM scope after three wonderful days at #SMLMS2022 #realtimemicroscopy
Thanks to Yoshi and @christmachu the TIRF-SIM is now on fleek #realtimemicroscopy
Nikon X Tokai X Ludin X Warner homemade collab is ready to play #realtimemicroscopy