Post-translational modifications of GlmR integrate metabolic and stress signals to maintain cell envelope homeostasis in Bacillus subtilis Author summary Bacteria must integrate feedback from multiple metabolic processes and environmental cues to efficiently allocate carbon to produce essential building blocks such as nucleotides, amino ...

🫵 Post-translational modifications of GlmR integrate metabolic and stress signals to maintain cell envelope homeostasis in Bacillus subtilis:

👇👇👇

dx.plos.org/10.1371/jour...

@cdiAMP #subtiwiki

YqeK governs the acquisition and removal of nucleoside tetraphosphate caps in Firmicutes Abstract. Nucleoside tetraphosphate (Np4) caps have heretofore been reported in only one bacterial species: the model Gram-negative organism Escherichia co

👉 YqeK governs the acquisition and removal of nucleoside tetraphosphate caps in Firmicutes:

academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki

Reversible DNA condensation drives natural transformation - Nature Communications The molecular mechanisms underlying natural transformation remain poorly understood. Here, the authors use optical tweezers to show how the periplasmic DNA receptor ComEA drives the inward pulling of ...

🫴 Reversible DNA condensation drives natural transformation:

www.nature.com/articles/s41...

@natcomms.nature.com #subtiwiki

Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding Abstract. The broadly conserved ParB protein performs crucial functions in bacterial chromosome segregation and replication regulation. The cellular functi

👉 Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding:

academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki

Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system - Nature Biotechnology Mutational rates of bacterial evolution are increased using an error-prone orthogonal system.

Highly mutagenic continuous evolution in Wolfgang Escherichia coli using a Φ29-based (Bacillus subtilis phage!) orthogonal replication system:

www.nature.com/articles/s41...

@natbiotech.nature.com #subtiwiki

Important new discovery: AsnS controls replication restart from DNA:RNA hybrids!
Houra Merrikh lab #SubtiWiki
@biorxiv-microbiol.bsky.social

@heyzajr.bsky.social

www.biorxiv.org/content/10.6...

For Bacillus subtilis aficionados: Who recognizes the people in the photos?

#subtiwiki #subtilis #MIT

The #SubtiWiki paper of the month for February has been chosen. It reports about AppppA-mediated control of protein acetylation. Congrats to the groups of @bangebalcony.bsky.social and collaborstors!

subtiwiki.uni-goettingen.de/wiki/index.p...

The next International Conference on Gram-Positive Bacteria will take place between 29 June – 2 July 2027 in Ljubljana, Slovenia.

#subtiwiki

Regulatory rewiring drives intraspecies competition in Bacillus subtilis Author summary Microbial competition and cooperation are key in shaping the structure and evolution of microbial communities. Our study on Bacillus subtilis, a model for microbial social interactions,...

Checkout this article we found at PLOS:

👉 Regulatory rewiring drives intraspecies competition in Bacillus subtilis!

dx.plos.org/10.1371/jour...

#subtiwiki

Validate User

👉 Diversity and distribution of bacterial DNA polymerases:

academic.oup.com/nar/article/...

#subtiwiki

@narjournal.bsky.social

A protein adaptor mediating Ap4A-dependent control of protein acetylation - Nature Communications This study identifies AcuB as a conserved inhibitor of the deacetylase AcuC in bacteria. AcuB directly suppresses AcuC activity, and this inhibition is enhanced the alarmone diadenosine tetraphosphate...

👉 A protein adaptor mediating Ap4A-dependent control of protein acetylation:

www.nature.com/articles/s41...

@natcomms.nature.com #subtiwiki

Oligomerization of fbaA Is Essential for Nonclassical Protein Export in Bacillus subtilis Bacillus subtilis is a widely used microbial host for both fundamental research and the industrial production of enzymes and biopharmaceuticals. However, its four known signal peptide–dependent secret...

Wow: Oligomerization of the glykolytic enzyme FbaA Is Essential for Nonclassical Protein Export in Bacillus subtilis:

| ACS Synthetic Biology pubs.acs.org/doi/10.1021/...

#subtiwiki

From now on, the #SubtiWiki interaction widget also includes interactions that involve RNA molecules (with proteins, metabolites, or other RNAs)! Please contact us if you miss any RNA interaction in B. subtilis!

The #SubtiWiki paper of the month for February has been selected. Great work on the final phase of cell division.

subtiwiki.uni-goettingen.de/wiki/index.p...

Phage infection fronts trigger early sporulation and viral entrapment in bacterial populations Abstract. Bacteriophage (phage) infect, lyse, and propagate within bacterial populations. However, physiological changes in bacterial cell state can protec

👉 Phage infection fronts trigger early sporulation and viral entrapment in bacterial populations:

academic.oup.com/ismej/articl...

#subtiwiki @spp2330.bsky.social

Cell wall hydrolysis promotes a second wave of transpeptidation to achieve cell separation following septation in Bacillus subtilis - Nature Communications Cell division in many Gram-positive bacteria involves the synthesis of a peptidoglycan septum that is then hydrolyzed to facilitate cell separation. Here the authors show that, after septum formation,...

🫴 Cell wall hydrolysis promotes a second wave of transpeptidation to achieve cell separation following septation in Bacillus subtilis:

👇👇👇

www.nature.com/articles/s41...

#subtiwiki

Can someone pinpoint if there is a Pseudomonas putida (e.g. KT2442) database where we can find out which lab has certain deletion mutants and/or report strains?

Coming from the B.subtilis field with #SubtiWiki containing all these info, it is difficult to find all available mutants in the papers

Starvation-independent alarmone production inhibits translation through GTP depletion:

www.biorxiv.org/content/10.6...

#subtiwiki (p)ppGpp

A DNA recognition-mimicry switch governs induction in arbitrium phages Temperate phages integrate multiple information sources to regulate lysis-lysogeny transitions. SPbeta-like phages use arbitrium signaling and DNA dam…

🫴 Fantastic work: DNA recognition-mimicry switch governs induction in arbitrium phages:

www.sciencedirect.com/science/arti...

@spp2330.bsky.social #subtiwiki @cp-cellhostmicrobe.bsky.social

Validate User

How do bacteria sense the antibiotic rifampicin? They employ a widespread dual-promoter based alarm system!

academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki

Advancing Fast-Track Genome Engineering in Bacillus subtilis Phages Phage genome engineering methods accelerate the study of phage biology, the discovery of new functions, and the development of innovative genetic engineering tools. Here, we present QuickPhage, a rapi...

A new tool for engineering lytic phages of the Gram-positive model bacterium Bacillus subtilis:

| ACS Synthetic Biology pubs.acs.org/doi/10.1021/...

#subtiwiki

A mechanotransduction mechanism for antibiotic defense in Gram-positive bacteria In this issue of Structure, Yu et al. show that the Bacillus subtilis ytr operon encodes two distinct ABC transporters. The authors present the cryo-EM structures of YtrEF in the apo and ADP-vanadate-...

🫴 A mechanotransduction mechanism for antibiotic defense in Gram-positive bacteria (Bacillus subtilis, with some interesting suppressor mutants):

In

Structure www.cell.com/structure/fu...

#subtiWiki #subtilis

Molecular characterisation of the Bacillus subtilis SpbK antiphage defence system - PubMed Bacteria have a variety of mechanisms for limiting predation by phages. SpbK is a Toll/interleukin-1 receptor (TIR) domain-containing antiphage defence protein from Bacillus subtilis that provides protection against the temperate phage SPβ via abortive infection. Here we structurally characterise Sp …

🫴 Molecular characterisation of the Bacillus subtilis SpbK antiphage defence system:

pubmed.ncbi.nlm.nih.gov/41462020/

@spp2330.bsky.social #subtilis #Subtiwiki @natcomms.nature.com

Metastable folding of Bacillus subtilis glmS ribozyme modulates turnover by RNase J1 Rapid turnover of glmS mRNA in Bacillus subtilis by 5′-3’ exoribonuclease RNase J is essential for feedback regulation of glucosamine-6-phosphate (Glc…

🫳 Metastable folding of Bacillus subtilis glmS ribozyme modulates turnover by RNase J1:

www.sciencedirect.com/science/arti...

#subtiwiki

😎 Today in Friday's seminar, a breakthrough was reported: Bacillus subtilis grows with Coca-Cola and energy drinks:

#subtiwiki #cola #energy

The FsrA‐Mediated Iron‐Sparing Response Regulates the Biosynthesis of the Epipeptide EPE in Bacillus subtilis Under iron-limited conditions, FsrA base-pairs with the intergenic region between epeX and epeE, enhancing epeE translation and triggering EPE production. Toxin-mediated cell lysis releases nutrients...

👉 The FsrA-Mediated Iron-Sparing Response Regulates the Biosynthesis of the Epipeptide EPE in Bacillus subtilis:

👇

Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...

@molecularmicro.bsky.social #subtiwiki

Our team wishes you a Merry Christmas and all the best for the new year!
@uni-goettingen.de #Subtiwiki

Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis How good is Bacillus subtilis as a model for the spore-forming pathogen Bacillus anthracis? Using high throughput genetics to identify B. anthracis sporulation genes and cytological analysis of the mu...

The Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis:

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dx.plos.org/10.1371/jour...

@plosbiology.org #subtiwiki

@joergstuelke.bsky.social #subtiwiki

Love the new structural homology feature on subtiwiki! Thank you!