For Bacillus subtilis aficionados: Who recognizes the people in the photos?

#subtiwiki #subtilis #MIT

A mechanotransduction mechanism for antibiotic defense in Gram-positive bacteria In this issue of Structure, Yu et al. show that the Bacillus subtilis ytr operon encodes two distinct ABC transporters. The authors present the cryo-EM structures of YtrEF in the apo and ADP-vanadate-...

🫴 A mechanotransduction mechanism for antibiotic defense in Gram-positive bacteria (Bacillus subtilis, with some interesting suppressor mutants):

In

Structure www.cell.com/structure/fu...

#subtiWiki #subtilis

Molecular characterisation of the Bacillus subtilis SpbK antiphage defence system - PubMed Bacteria have a variety of mechanisms for limiting predation by phages. SpbK is a Toll/interleukin-1 receptor (TIR) domain-containing antiphage defence protein from Bacillus subtilis that provides protection against the temperate phage SPβ via abortive infection. Here we structurally characterise Sp …

🫴 Molecular characterisation of the Bacillus subtilis SpbK antiphage defence system:

pubmed.ncbi.nlm.nih.gov/41462020/

@spp2330.bsky.social #subtilis #Subtiwiki @natcomms.nature.com

Staying in the loop to make ends meet: roles and regulation of GlmR in Bacillus subtilis The metabolic networks of most life forms integrate cost-benefit analysis to properly budget carbon and other essential nutrients through continuous assessment of nutrient availability and environment...

🥸 Staying in the loop to make ends meet: roles and regulation of GlmR in Bacillus subtilis:

www.biorxiv.org/content/10.1...

#subtiwiki #cdiAMP #subtilis

Cross-kingdom RNA trafficking from bacteria to fungi enables plant protection against fungal pathogens In this study we engineered gram-positive and gram-negative beneficial bacteria to produce double-stranded RNAs targeting essential genes in important fungal pathogens. We demonstrate that RNAs can be...

Great: Cross-kingdom RNA trafficking from bacteria (including Bacillus subtilis) to fungi enables plant protection against fungal pathogens!

👇

Molecular Plant www.cell.com/molecular-pl...

#subtiwiki #subtilis

Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization Vitamin B6 refers to a family of water-soluble B vitamin, which is essential for various physiological functions, including amino acid metabolism, neu…

🫴 Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization:

www.sciencedirect.com/science/arti...

#vitaminB6 #subtilis

ComFB, a widespread family of c-di-NMP receptor proteins | PNAS Cyclic dimeric-GMP (c-di-GMP) is a ubiquitous bacterial second messenger that regulates a variety of cellular processes, including motility, biofil...

🫴 Wow: ComFB is a widespread family of c-di-NMP receptor proteins!

👇👇👇

www.pnas.org/doi/10.1073/...

@pnas.org #subtiwiki #subtilis

Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation | mSystems Understanding how bacteria survive extreme conditions is key to tackling challenges in health, food safety, and industry. This study reveals a previously unexplored layer of control in Bacillus subtil...

Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...

Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome Abstract. Recent evidence indicates that the bacterial Rho helicase regulates Bacillus subtilis gene expression in a growth-dependent manner. This regulati

🫴 Fantastic material for our special lecture "molecular microbiology":

Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome url: academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki #subtilis

A ptsH mutation suppresses growth defects and antibiotic sensitivity in a cpgA mutant defective in metabolite proofreading | Journal of Bacteriology Metabolism relies on the concerted action of hundreds of enzymes, many of which have some activity with non-canonical substrates. The resulting reactions constitute an often-ignored underground metabolism. Glyceraldehyde-3-phosphate dehydrogenase ...

A ptsH mutation (encoding the HPr-G54D variant) suppresses growth defects and antibiotic sensitivity in a Bacillus subtilis cpgA mutant defective in metabolite proofreading!

Where? In Journal of Bacteriology!

🫳 journals.asm.org/doi/10.1128/...

#subtiwiki #subtilis #CCR

Expression level of anti-phage defence systems controls a trade-off between protection range and autoimmunity - Nature Microbiology The authors examine several defense systems and find that increased expression enhances their protection range, albeit at a cost of autoimmunity.

🫴 Expression level of anti-phage defence systems controls a trade-off between protection range and autoimmunity:

www.nature.com/articles/s41...

#subtilis #subtiwiki #SPbeta @natmicrobiol.nature.com

🫴 The Monofunctional FMN Riboswitch-Binding Regulator RibR From Bacillus amyloliquefaciens Promotes Riboflavin Biosynthesis

@molecularmicro.bsky.social #subtilis #subtiwiki

Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/...

Characterization of the MgsR-dependent promoter structure in Bacillus subtilis—application of a novel pHIS plasmid-based screening system for promoter element analysis Abstract. Transcriptional control is a finely tuned process, crucial for adaptation, differentiation, and survival. However, deciphering the contribution o

🫴 Characterization of the MgsR-dependent promoter structure in Bacillus subtilis—application of a novel pHIS plasmid-based screening system for promoter element analysis:

academic.oup.com/nar/article/...

@narjournal.bsky.social #subtiwiki #subtilis

Also doch der Turgor!

Great paper from David Rudner's laboratory!

#cdAMP #subtiwiki #subtilis

The SinR•SlrR heteromer attenuates transcription of a long operon of flagellar genes in Bacillus subtilis During growth, Bacillus subtilis differentiates into subpopulations of motile individuals and non-motile chains, associated with dispersal and biofilm…

🫴 The SinR•SlrR heteromer attenuates transcription of a long operon of flagellar genes in Bacillus subtilis

www.sciencedirect.com/science/arti...

#subtiwiki #subtilis

A phage-encoded counter-defense inhibits an NAD-degrading anti-phage defense system Author summary Bacterial viruses (bacteriophages or phages) are widespread and abundant across the planet. Bacteria have a variety of immune systems, often found on mobile genetic elements, to combat ...

👉 ICEBs1 protects against SPβ infection but Φ3T has a counter-defense gene!

Checkout this article we found at PLOS: dx.plos.org/10.1371/jour...

#subtilis #SPβ #Φ3T #subtiwiki

Structural basis of directionality control in large serine integrases Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF...

🫴 Structural basis of directionality control in large serine integrase from temperate Bacillus subtilis phage SPbeta:

www.biorxiv.org/content/10.1...

#subtiwiki #subtilis #integration #excision

Dynamics of an algae–bacteria microcosm: Photosynthesis, chemotaxis, and expulsion in inhomogeneous active matter | PNAS In nature, there are significant relationships known between microorganisms from two kingdoms of life, as in the supply of vitamin B12 by bacteria ...

🥸 The resulting generalization of Fick's law has been proposed in the context of chemotaxis and is mathematically related to the "turbulent pumping" in magnetohydrodynamics:

www.pnas.org/doi/10.1073/...

#subtiwiki #subtilis @pnas.org

Phosphorelay changes and plasticity underlie the life history evolution of Bacillus subtilis sporulation and germination in serial batch culture Bacterial endospores facilitate survival in extreme and unpredictably fluctuating environments. However, under abundant nutrient conditions, the production of endospores is quickly reduced or lost. We...

🫴 Phosphorelay changes and plasticity underlie the life history evolution of Bacillus subtilis sporulation and germination in serial batch culture:

www.microbiologyresearch.org/content/jour...

@microbiologysociety.org #subtilis #subtiwiki

Regulation of acetyl-CoA biosynthesis via an intertwined acetyl-CoA synthetase/acetyltransferase complex - Nature Communications Here the authors investigate the regulatory mechanisms of acetyl-CoA (Ac-CoA) biosynthesis in Bacillus subtilis, probing the interaction between acetyl-CoA synthetase (AcsA) and acetyltransferase (Acu...

🫴 Mechanistic insights into the regulatory framework underlying acetyl-CoA biosynthesis from acetate in Bacillus subtilis:

👇👇👇

www.nature.com/articles/s41...

@bangebalcony.bsky.social #subtiwiki #subtilis @naturecomms.bsky.social

Pyruvate kinase directly generates GTP in glycolysis, supporting growth and contributing to guanosine toxicity | mBio In this study, we reveal that pyruvate kinase, a key glycolytic enzyme, primarily generates GTP from GDP in Bacillus subtilis, relative to other nucleotide triphosphates, such as ATP. This finding, uncovered through genetic selection for mutants that ...

WOW!

🫴 Pyruvate kinase directly generates GTP in glycolysis, supporting growth and contributing to guanosine toxicity

👇

journals.asm.org/doi/10.1128/...

@asm.org #subtilis #subtiwiki

🫴 Next BACELL meeting:

bacell2025.eu

#subtilis #subtiwiki #amsterdam

Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis - Nature Communications Some bacteria use a large protein complex, the stressosome, to sense and respond to environmental stress. Here, Khadka et al. show that a long-assumed sensor in the complex is dispensable for sensing,...

🫴 Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis

👇

www.nature.com/articles/s41...

@naturecomms.bsky.social #subtilis #stress

A mutation in RNA polymerase imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism Patel and Helmann identify metabolic pathways that are dysregulated in RNAP mutant strains with altered β-lactam sensitivity. Genetic and physiological studies demonstrate that BCAA and Pyr synthesis ...

🫴 A mutation in RNA polymerase imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism: Now in Cell Reports by @johnhelmann.bsky.social

👇

www.cell.com/cell-reports...

#subtiwiki #subtilis @cellpress.bsky.social

A screenshot of the results table of a Foldseek search on SubtiWiki. It shows multiple entries and corresponding metrics. One of the rows is expanded, revealing a 3D visualization of a superimposition of the target and the query structure corresponding to the search hit.

Try out our integration of the amazing protein structure search tool #Foldseek on #SubtiWiki (subtiwiki.uni-goettingen.de/foldseek)! Just upload a structure or use PDB/AlphaFoldDB entries to find similar structures. #IMPRS-GS #UniGoettingen
#alphafold #bacillus #subtilis

#Biocontrol of the #wheat pathogen #Zymoseptoria tritici using cyclic #lipopeptides from #Bacillus #subtilis

link.springer.com/article/10.100…