⏪ G&D RECAP ⏪

🗓️ April 2025
OUTLOOK: Where and why have so many metabolic enzymes gone from developing spores of Bacillus subtilis?
By Peter Setlow
➡️ https://genesdev.cshlp.org/content/39/7-8/426.full

#bacteria #sporulation #protease #metabolism #dormancy

⏪ G&D RECAP ⏪

🗓️ April 2025
RESEARCH PAPER: MdfA is a novel ClpC adaptor protein that functions in the developing Bacillus subtilis spore
By Massoni et al., Rivka Isaacson, and Amy Camp
➡️ https://genesdev.cshlp.org/content/39/7-8/510.full

#bacteria #sporulation #protease #metabolism #dormancy

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

(PDF) Impact of temperature on vegetative and pathogenic development of the fungal multi-host pathogen Lasiodiplodia theobromae (Pat.) Griffon & Maubl. PDF | Lasiodiplodia theobromae is an economically important multi-host plant pathogen infecting nearly 500 hosts, including perennial crops. In Peru, L.... | Find, read and cite all the research you n...

Impact of temperature on vegetative and pathogenic development of the fungal multi-host pathogen Lasiodiplodia theobromae (Pat.) Griffon & Maubl.

#Lasiodiplodia
#dieback
#sporulation
#temperature
#virulence

www.researchgate.net/publication/...

Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation | mSystems Understanding how bacteria survive extreme conditions is key to tackling challenges in health, food safety, and industry. This study reveals a previously unexplored layer of control in Bacillus subtil...

Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...

Interested in #Cdiff #BugSlayers #sporulation and interdisciplinary research combining #microbiology #structuralbiology and #biochemistry? Please get in touch about possibilities to join us in #Salgadolab. #microSky

Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has ...

🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social

Scientists discover protein key to bacteria's survival in extreme environments Scientists have discovered a protein that enables bacteria to shut down into dormant spores under extreme conditions. The process, which enables the bacteria to become practically indestructible, expl...

Scientists discover #protein key to #bacteria's survival in extreme environments.

#extremophiles #sporulation #Bacillus #metabolism #protein #MdfA #nzymes #ClpC

phys.org/news/2025-03...

Genomic and phenotypic characterization of a Clostridioides difficile strain of the epidemic ST37 type from China - PubMed <span><i>Clostridioides difficile</i> strains of sequence type (ST) 37, primarily including PCR ribotype (RT) 017, are prevalent in mainland China. Our study aimed to compare the major virulence facto...

Our group contributed to a recent manuscript by Xingmin Sun's group; an important study on #Cdiff RT017/ST37, which is a dominant type in Asia #sporulation #toxinproduction #adherence #mousemodel pubmed.ncbi.nlm.nih.gov/39492989/

Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile Molecular Microbiology is a broad-scope microbiology journal for basic and translational research that contributes to understanding molecular principles in microbes.

Great to be part of new paper from Monica & Adriano labs
at ITQB-Nova on SpoIVB2 protease in #Cdiff #sporulation, its impact on SpoIIP activity (Charlotte Roughton, Salgado lab) and in vivo cleavage (Hailee, @thejoesorg.bsky.social lab). Congratulations everyone!
doi.org/10.1111/mmi....

MSCA opens €417 million call for Postdoctoral Fellowships Postdoctoral Fellowships offer researchers holding a PhD the opportunity to gain new skills and experience while carrying out their own research project abroad. The deadline to apply is 11 September 2...

If you would like to join the #Salgadolab work on #Cdiff, #BugSlayers and #sporulation using a range of techniques from #structuralbiology, #biochemistry, #microbiology - get in touch! Happy to discuss and support applications!

2024 #MSCA Postdoctoral Fellowships call is open!
europa.eu/!8TwbVX

Cell division machinery drives cell-specific gene activation during differentiation in Bacillus subtilis
#sporulation

PNAS from @ramamurthilab.bsky.social

www.pnas.org/doi/10.1073/...