Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in Bacillus subtilis

bioRxix from Stephen C Jacobson
#Bsubtilis #CellBiology #MicroSky

Starvation-independent alarmone production inhibits translation through GTP depletion
- bioRxiv from @heatherfeaga.bsky.social
#ppGpp #Bsubtilis

Evolutionary bioenergetics of sporulation | PNAS Energy is required for the expression and maintenance of complex traits. In many habitats, however, free energy available to support biosynthesis i...

Evolutionary bioenergetics of sporulation

"energetic cost of sporulation in units of ATP by integrating time-resolved genome, transcriptome, and proteome profiles"
#Bsubtilis #MicroSky

#PNAS by @ckarakoc.bsky.social @shoestrapped.bsky.social @jaytlennon.bsky.social

www.pnas.org/doi/10.1073/...

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

Conserved B. anthracis sporulation genes have mutant phenotypes that are similar, but not identical, to B. subtilis mutants. Top: Representative fluorescence and phase-contrast micrographs of sporulating B. anthracis and B. subtilis. Wild-type strains were induced to sporulate by nutrient exhaustion at 37 °C in PA (B. anthracis) or DSM (B. subtilis). Time after the onset of sporulation is indicated in the top left corner of the B. anthracis images. The membranes were stained (0–5 hour) with TMA-DPH. Bottom: Representative fluorescence images of the indicated B. anthracis mutants at hour 4 of sporulation. All strains harbor forespore (PspoIIQ-yfp) and mother cell (PspoIID-cfp) transcriptional reporters. σF activity in the forespore (SigF) and σE activity in the mother cell (SigE) were visualized with YFP (false-colored green) and CFP (false-colored red), respectively. The membranes were stained with TMA-DPH. Disporic cells in spoIIR::Tn (yellow carets), impaired engulfment in spoIID::Tn and spoIIQ::Tn (white carets), and small forespores in spoIIIAE::Tn (light blue carets) are highlighted. Scale bars indicate 5 μm.

Is #Bsubtilis a good model for the spore-forming #anthrax #pathogen B. anthracis? This study identifies >150 B. anthracis #sporulation genes and cytologically phenotypes the mutants, revealing similarities & striking differences between model & pathogen @plosbiology.org 🧪 plos.io/3YsvlyZ

Green synthesis of silver nanoparticles using Ageratum conyzoides for activated carbon compositing to prepare antimicrobial cotton fabric Wearing socks or shoes for an extended time can result in the growth of microorganisms and associated foot odor, which affects foot hygiene. Therefore, the development of an antimicrobial cloth layer…

Scientists fabricated PCFs@HAC-AgNPs using #AgNPs from ACLE, coated on PCFs, which demonstrated strong #antibacterial activity against #Saureus and #Bsubtilis, and potential as sustainable odor-reducing #ShoeComponents.

#OpenAccess in Nanotechnology Reviews:

A genetic screen reveals key residues and new insights into the polymerization cycle of MreB - Arnaud Chastanet
#CellWall of #Bsubtilis
#BACELL2025

Saurabh Bhattacharya (Sigal Ben Yehuda group)- The native molecular cargo transported via interspecies bacterial nanotubes

#Bsubtilis #nanotubes

#MultiBac2025