Many processes influence boundary sharpness and placement: gene regulatory interactions, spatial averaging, cell sorting to name a few.
Our analysis shows that cellular readout noise is the main determinant of boundary sharpness, i.e. #TZW.
Preprint: doi.org/10.64898/202...
🧵 6/6
By matching simulations with measurement of #TZW & #positional #error, we inferred kinetic and readout noise levels - and found them in the reported range.
This further supports that reliable long-range morphogen patterning is feasible with physiological noise levels:
x.com/DagmarIber/s...
🧵 5/6
We uncover a trade-off regarding cell size:
• Larger cells yield sharper boundaries (smaller #TZW)
• Smaller cells reduce variability in boundary position between embryos (lower positional error)
The measured cell size in the neural tube perfectly balances boundary sharpness and precision.
🧵 4/6
Our theoretical & computational analysis shows that #TZW is primarily set by #noise in the #cellular #readout process, not by fluctuations in the morphogen gradient itself.
For exponential gradients, a noisy readout threshold naturally yields a position-independent TZW.
🧵 3/6
According to prevailing theory, transition zones should widen exponentially with distance from the morphogen source, due to stochastic effects at low morphogen copy numbers.
#Contrary, we find that #TZW remains about #constant, independent of readout position and developmental timepoint.
🧵 2/6
Many processes influence boundary sharpness and placement: gene regulatory interactions, spatial averaging, cell sorting to name a few.
Our analysis shows that cellular readout noise is the main determinant of boundary sharpness, i.e. #TZW.
Preprint: doi.org/10.64898/202...
🧵 6/6
By matching simulations with measurement of #TZW & #positional #error, we inferred kinetic and readout noise levels - and found them in the reported range.
This further supports that reliable long-range morphogen patterning is feasible with physiological noise levels:
x.com/DagmarIber/s...
🧵 5/6
It's here! The latest episode of #TZW TV, WAR 2-8! The TIGER is finally revealed! But what is her history with either Rarity #friendshipismagic or Garnet #stevenuniverse? This and more!
www.deviantart.com/kingfezprodu...
That time the late Rob Ford (while Mayor of Toronto) dropped in for a bite at the Zombie Walk (with Mike Gohm). #Toronto #Zombie #WalkingDead #RobFord #TZW #TorontoZombieWalk #CrackMayor
