Our Simple Animal & Plant #cell paper models make it easy to learn the parts of #cells & see the similarities & differences! More here: buff.ly/RIsh8JE
#EduSky #nucleus #mitochondria #chloroplast #endoplasmicreticulum #vacuole #cellmembrane #cellwall #animalcell #plantcell
Description: Colocalization of AtFH1-mScarlet-I and the tonoplast marker VHP1:mGFP Source: Fiugure from the Original study
#Arabidopsis rocks again in #research! 🌱 We know Class I formin AtFH1 affects #plant #cell architecture. But disrupting AtFH1 alters #vacuole structure and dynamics, hinting it’s an active cargo affecting the #tonoplast on its way out!
Read more ⬇️
🔗 www.frontiersin.org/journals/pla...
(TŽ)
This week #JournalClub @labsoap.bsky.social @science.org #LYVAC #vacuole #lysosome www.science.org/doi/10.1126/...
New lab / new life 🌱
The Julian Lab is now open at the Institute of Molecular Plant Biology (IMPB), BOKU University 🇦🇹
We explore how plants defend their vacuoles and how vacuoles defend them! 🛡️
Excited to collaborate with @angegross.bsky.social’s lab.
#PlantBiology #Vacuole #VQC #BOKU #IMPB
Novel reporters for inclusion damage revealed instability of CpoS-deficient inclusions. Top left: Schematic showing the principle of detecting inclusion damage using the split-GFP approach. Right: Fluorescence microscopic detection of inclusion damage during infection with CTL2-cpoS::cat. HeLa cells were transfected with a plasmid driving GFP1–10 expression, infected with the indicated strains (5 IFU/cell), and then fixed, stained (DNA (Hoechst) staining), and imaged at 26 hpi (scale = 20 µm). Bottom left: FIB-SEM analysis validating inclusion damage at the ultrastructural level. GFP1–10-expressing HeLa cells were infected with the indicated strain and fixed at 24 hpi. A cell containing green-fluorescent bacteria was identified by fluorescence microscopy and subjected to FIB-SEM analysis (scale = 1 µm, green arrows highlight bacteria in the host cell cytosol).
Intracellular pathogens like #Chlamydia can hide from host defenses in a #vacuole. This study shows that CpoS maintains the stability of Chlamydia’s parasitophorous vacuole; early vacuole destabilization clears infection, suggesting a potential therapeutic target @plosbiology.org 🧪 plos.io/3Ur0YqJ
Novel reporters for inclusion damage revealed instability of CpoS-deficient inclusions. Top left: Schematic showing the principle of detecting inclusion damage using the split-GFP approach. Right: Fluorescence microscopic detection of inclusion damage during infection with CTL2-cpoS::cat. HeLa cells were transfected with a plasmid driving GFP1–10 expression, infected with the indicated strains (5 IFU/cell), and then fixed, stained (DNA (Hoechst) staining), and imaged at 26 hpi (scale = 20 µm). Bottom left: FIB-SEM analysis validating inclusion damage at the ultrastructural level. GFP1–10-expressing HeLa cells were infected with the indicated strain and fixed at 24 hpi. A cell containing green-fluorescent bacteria was identified by fluorescence microscopy and subjected to FIB-SEM analysis (scale = 1 µm, green arrows highlight bacteria in the host cell cytosol).
Intracellular pathogens like #Chlamydia can hide from host defenses in a #vacuole. This study shows that CpoS maintains the stability of Chlamydia’s parasitophorous vacuole; early vacuole destabilization clears infection, suggesting a potential therapeutic target @plosbiology.org 🧪 plos.io/3Ur0YqJ
Novel reporters for inclusion damage revealed instability of CpoS-deficient inclusions. Top left: Schematic showing the principle of detecting inclusion damage using the split-GFP approach. Right: Fluorescence microscopic detection of inclusion damage during infection with CTL2-cpoS::cat. HeLa cells were transfected with a plasmid driving GFP1–10 expression, infected with the indicated strains (5 IFU/cell), and then fixed, stained (DNA (Hoechst) staining), and imaged at 26 hpi (scale = 20 µm). Bottom left: FIB-SEM analysis validating inclusion damage at the ultrastructural level. GFP1–10-expressing HeLa cells were infected with the indicated strain and fixed at 24 hpi. A cell containing green-fluorescent bacteria was identified by fluorescence microscopy and subjected to FIB-SEM analysis (scale = 1 µm, green arrows highlight bacteria in the host cell cytosol).
Intracellular pathogens like #Chlamydia can hide from host defenses in a #vacuole. This study shows that CpoS maintains the stability of Chlamydia’s parasitophorous vacuole; early vacuole destabilization clears infection, suggesting a potential therapeutic target @plosbiology.org 🧪 plos.io/3Ur0YqJ
Dr Noelia Lander reviews the structure of #cAMP compartments in #Trypanosoma #cruzi. #Contractile #Vacuole #Environmental #Sensing #Flagellar #Distal #Metacyclogenesis #Osmoregulation #Signaling #Microdomains
authors.elsevier.com/a/1lRke5Eb1x...
🆕 Review by @josejulian.bsky.social on one of my favourite organelles: #vacuole (signaling & biogenesis & quality control) www.sciencedirect.com/science/arti... There's so much exciting stuff waiting to be discovered on these mammoth organelles!
Bacterial #pathogens hijack host #cell #peroxisomes for replication #vacuole expansion and integrity
#virulence @hosmic.bsky.social @mikrobiokosmos.bsky.social
doi.org/10.1126/scia...
For the first time in plants, we used APEX-based electron microscopy to map the precise localization of ATG8 at the vacuolar membrane after stress! 🚀
Pushing the boundaries of plant cell biology—one EM image at a time. ⚡👀
#PlantScience #ElectronMicroscopy #Autophagy #Vacuole #NaturePlants
