ChimeraX helps us handles so many chains with the doublet microtubule #WeNeedChimeraX

So... #WeNeedChimeraX and we need to support it generously so it can evolve faster, give us new ways to discover drug targets, new ways to understand drug binding, etc.

Without tools like this, all the structural biology we fund risks creating results, but not knowledge.

I want to add that I've been witnessing first-hand how the ChimeraX people are incredibly reactive and helpful on the user mailing list. Keep up the good work and let us know how we can help #WeNeedChimeraX

Mechanistic Determinants of Slow Axonal Transport and Presynaptic Targeting of Clathrin Packets Clathrin has established roles in endocytosis, with clathrin-cages enclosing membrane infoldings, followed by rapid disassembly and reuse of monomers. However, in neurons, clathrin synthesized in cell-bodies is conveyed into axons and synapses via slow axonal transport; as shown by classic pulse-chase radiolabeling. What is the cargo-structure, and mechanisms underlying transport and presynaptic-targeting of clathrin? What is the precise organization at synapses? Combining live-imaging, mass-spectrometry (MS), Apex-labeled EM-tomography and super-resolution, we found that unlike dendrites where clathrin transiently assembles/disassembles as expected, axons contain stable ‘transport-packets’ that move intermittently with an anterograde bias; with actin/myosin-VI as putative tethers. Transport-packets are unrelated to endocytosis, and the overall kinetics generate a slow biased flow of axonal clathrin. Synapses have integer-numbers of clathrin-packets circumferentially abutting the synaptic-vesicle cluster, advocating a model where delivery of clathrin-packets by slow axonal transport generates a radial organization of clathrin at synapses. Our experiments reveal novel trafficking mechanisms, and an unexpected nanoscale organization of synaptic clathrin.

#WeNeedChimeraX I repped ChimeraX yesterday during my webinar - started a few months ago using it to push the visualization of our 3D super-resolution microscopy to the next level. Here are a few microns of axon with ~100 nm clathrin clusters inside. More: https://t.co/iKpdcx9Crv https://t.co/ci

#CryoEM map of human TFIIH in #ChimeraX. Pretty, no? #WeNeedChimeraX!

Great visualisation tools such as ChimeraX are indispensable for structural biologists! #WeNeedChimeraX