2) However, my beloved model microbe #Ecoli doesn’t need glyoxylate for growth - it is nonessential. How do you design a strain that is #auxotrophic for such a metabolite ? Easy, you “just” rewire the metabolism, so that biomass precursor must be made from #glyoxylate!

Dear #Streptomyces community, Jana (@janitensen.bsky.social) and I want to test the evolvability/plasticity of Streptomyces metabolic networks. We plan to use #auxotrophic strains for this. If you have some and are willing to share, please get in touch with us! Thank you!

To implement these solutions, we can use #auxotrophic metabolic sensors, coupling growth to target metabolic module activity. In this #preprint co-led with Helena Schulz, we develop a suite of glyoxylate and glycolate sensors with wide-range sensitivity & different scopes. 🔬🌱
doi.org/10.1101/2024...