5) Next, we characterised the strains by verifying expected metabolic fluxes by isotope tracing and determining their selective demands with #glyoxylate and #glycolate.
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Hashtag
#glyoxylate
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2) However, my beloved model microbe #Ecoli doesn’t need glyoxylate for growth - it is nonessential. How do you design a strain that is #auxotrophic for such a metabolite ? Easy, you “just” rewire the metabolism, so that biomass precursor must be made from #glyoxylate!
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1) #Glyoxylate is a key intermediate of synthetic #CO2fixation metabolism - for example, it is the product of the CETCH, the HOPAC or the MOG cycles. To implement those designs in vivo, we want to couple cell growth to those synthetic pathways.
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