L2/3 pyramidal cell type composition and gene expression are selectively regulated during development. Top: Scatter plot of PC1 vs. PC2 for L2/3 neurons at stages P12 (top row) and P22 (bottom row). Within each row, the leftmost panel highlights cells colored by their type-identity (L2/3_A, L2/3_B, and L2/3_C). In the remaining three panels within each row, cells are colored based on their aggregate expression levels of markers for each type. Between P12 and P22, L2/3_A markers decrease in expression and increase in specificity, while L2/3_B markers increase in expression during development, driving cell type identity maturation. Bottom: Representative FISH images showing labeling of cell type markers Cdh13, Trpc6, and Chrm2 at P12 (top row) and P22 (bottom row) within whisker somatosensory cortex L2/3. Arrowheads indicate putative Cdh13-expressing interneurons. Righthand images show a merge of all three labels.

A transcriptomic atlas of the developing mouse #BarrelCortex reveals that sensory experience only minimally affects neuronal cell type maturation, and that conserved molecular mechanisms underlie cortical development & plasticity @karthikshekhar.bsky.social &co in @plosbiology.org 🧪 plos.io/43u4XrM

L2/3 pyramidal cell type composition and gene expression are selectively regulated during development. Top: Scatter plot of PC1 vs. PC2 for L2/3 neurons at stages P12 (top row) and P22 (bottom row). Within each row, the leftmost panel highlights cells colored by their type-identity (L2/3_A, L2/3_B, and L2/3_C). In the remaining three panels within each row, cells are colored based on their aggregate expression levels of markers for each type. Between P12 and P22, L2/3_A markers decrease in expression and increase in specificity, while L2/3_B markers increase in expression during development, driving cell type identity maturation. Bottom: Representative FISH images showing labeling of cell type markers Cdh13, Trpc6, and Chrm2 at P12 (top row) and P22 (bottom row) within whisker somatosensory cortex L2/3. Arrowheads indicate putative Cdh13-expressing interneurons. Righthand images show a merge of all three labels.

A transcriptomic atlas of the developing mouse #BarrelCortex reveals that sensory experience only minimally affects neuronal cell type maturation, and that conserved molecular mechanisms underlie cortical development & plasticity @karthikshekhar.bsky.social &co in @plosbiology.org 🧪 plos.io/43u4XrM

L2/3 pyramidal cell type composition and gene expression are selectively regulated during development. Top: Scatter plot of PC1 vs. PC2 for L2/3 neurons at stages P12 (top row) and P22 (bottom row). Within each row, the leftmost panel highlights cells colored by their type-identity (L2/3_A, L2/3_B, and L2/3_C). In the remaining three panels within each row, cells are colored based on their aggregate expression levels of markers for each type. Between P12 and P22, L2/3_A markers decrease in expression and increase in specificity, while L2/3_B markers increase in expression during development, driving cell type identity maturation. Bottom: Representative FISH images showing labeling of cell type markers Cdh13, Trpc6, and Chrm2 at P12 (top row) and P22 (bottom row) within whisker somatosensory cortex L2/3. Arrowheads indicate putative Cdh13-expressing interneurons. Righthand images show a merge of all three labels.

A transcriptomic atlas of the developing mouse #BarrelCortex reveals that sensory experience only minimally affects neuronal cell type maturation, and that conserved molecular mechanisms underlie cortical development & plasticity @karthikshekhar.bsky.social &co in @plosbiology.org 🧪 plos.io/43u4XrM

FXR1 Deletion from Cortical Parvalbumin Interneurons Modifies their Excitatory Synaptic Responses Fragile X autosomal homolog 1 (FXR1), a member of the fragile X messenger riboprotein 1 family, has been linked to psychiatric disorders including autism and schizophrenia. Parvalbumin (PV) interneuro...

First @bsky.app post to share my second, second author paper! Congrats to Dr. Scheuer for being #PhDone (with the first PhD at least). Have a look at #parvalbumin Interneurons in #barrelcortex after #FXR1 deletion!🐁
@uwmadison.bsky.social @sfnjournals.bsky.social
doi.org/10.1523/ENEURO.0363-24.2024

rodents have a region of the somatosensory cortex that was named the #barrelcortex after its shape, and which contains the barrel field. The 'barrels' of the barrel field stain darker than neighbouring regions (within cortical layer IV) when stained to reveal the presence of cytochrome c oxidase. 🧵2