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#eSRRF
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🚨 Important detail in the #ExM & #eSRRF protocol: #eSRRF is not only available as plugin in #ImageJ /Fiji 🌴, #NanoPyx also brings it into the #python world 🐍: doi.org/10.1038/s415...
How to get started? Check out the training manual: doi.org/10.5281/zeno...

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It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw

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🧐 How can I make sure my sample expands properly and how do I find the right eSRRF processing parameters?
Here you will find all the nitty-gritty details on #ExM sample preparation, 🔬 & #eSRRF processing 🏄, common pitfalls 🪨 and troubleshooting advice🛠️.

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🚨 #ExM meets #eSRRF 🔬🏄🌊 the full protocol now in ‪@natprot.nature.com‬: doi.org/10.1038/s415...

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I‘m already on my way to Prag for @embo.org #SLM2025 #Super-resolution practical course! Looking forward to contribute with live-cell #eSRRF 🏄‍♀️ , meet some familiar faces and many new ones 🤩. Here some impressions from the 🚂: Öresund, Berlin, Dresden & the Bastei.

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Exciting news! 🎉 I’m thrilled to join the @pnordenfelt.bsky.social Lab @lunduniversity-med.bsky.social as a postdoc! I’ll be working on bacterial infections using #DDM and #SuperResolution imaging, uncovering new insights into host-pathogen interactions. 🔬🦠 #PostdocLife #Microbiology #eSRRF 🏄‍♀️

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🚨🔬🤖📜 New paper! Introducing #LiquidEngine and #NanoPyx - #AI for accelerating #microscopy that explores how to maximise performance 🚀. Brainchild of
Bruno, @inesmcunha.bsky.social and António. Adventure with @guijacquemet.bsky.social et al. Faster #SRRF & #eSRRF!!

📄: www.nature.com/articles/s41...

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Too much or too little? Pursuing the optimal balance in super-resolution microscopy.

Too much or too little 🤷 ❓Getting the best of 🤝 image fidelity✨ & resolution 📌, is a matter of balance in super-resolution microscopy ⚖️🧘‍♀️🔬… and the cause of🧑🏻‍🔬👉 👨‍🦳. #eSRRF ’s data-driven parameter optimization comes to the rescue🛟 t.co/TY3o4xZPzI

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Some #eSRRF in action @henriqueslab.bsky.social @christlet.bsky.social

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Over the moon to see #eSRRF out! Another chapter in our great collaboration with @henriqueslab.bsky.social and all the gang. Special shoutout to NeuroCyto postdoc @terrymosoly who used #eSRRF to super-resolve microtubules in living neurons!
www.nature.com/articles/s41...

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Microscopes come in all flavours 🌶️, we did our best to try many 🔬! A shoutout to the #eSRRF team 🌍: Simao Coelho, Damian Martinez, @HenriquesLab.bsky.social 🇵🇹, Theresa Wiesner, Angelique Jimenez, @christlet.bsky.social 🇫🇷, Jonathan Nixon-Abel 🇬🇧🇺🇸,…

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Delighted to have contributed to #eSRRF ! Beautiful and incredibly useful work by
@henriqueslab.bsky.social
. Congratulations to
@hannah-superres.bsky.social
Romain Laine and everyone else involved 🚀🚀🔬🔬

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No more tweaking parameters to find the best results, #SQUIRREL 🐿️ will tell you the optimal parameter set ✨Sounds like a great idea? Romain Laine, Siân Culley and @henriqueslab.bsky.social made it happen with #eSRRF!

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🧵Have you ever tried #SRRF? Yes… then you are gonna 😍 this! No?… What took you so long? 😁 Finally #eSRRF is published @naturemethods! We are taking live-cell 3D #superresolution to the next level🏄‍♀️ 🔬: www.nature.com/articles/s41...

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Forgot to tag the #MiFoBio2023 fam - perfect timing for #eSRRF being out! https://www.nature.com/articles/s41592-023-02057-w

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Special shoutout to NeuroCyto postdoc @terrymosoly from the lab who used #eSRRF to super-resolve microtubules in living neurons!
https://www.nature.com/articles/s41592-023-02057-w
@mrs_imaging_amu @CNRSbiologie @inp_marseille

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Over the moon to see #eSRRF out @naturemethods! Another chapter in our great collaboration with @HenriquesLab and all the gang. Check @Hannah_SuperRes's short thread below ⬇️
twitter.com/Hannah_SuperRes/status/1...

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Amazing talk by @HenriquesLab ! Fully convinced to try #eSRRF on some live cell SAF imaging …!

x.com/HenriquesLab/s…

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