Quería shipeo con su crush y sentir love bombing, pero fue tan creepy y poco aesthetic que la red flag le hizo funarla. Se sentía cringe, pero el bro supo que la culpa era del hype y se fue de chill, porque él era BAE y le hizo ghosting a la vergüenza. Al final, el mejor POV fue LMAO.

#microflow

NAGASE Industry Invests in MiChS: Advancing Microflow Synthesis Technology NAGASE Industry has acquired shares of MiChS to enhance its capabilities in high-value chemical product development and supply.

NAGASE Industry Invests in MiChS: Advancing Microflow Synthesis Technology #Japan #Tokyo #NAGASE #MiChS #Microflow

Tomorrow I’m giving a webinar explaining all of our story with @sergeych.bsky.social on #microflow #multinozzle ESI and how we managed to get more with less 😉 #lipidomics #metabolomics folks please share (and attend 😄)

Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography Mass Spectrometry (Unified-HILIC/AEX/MS): A Single-Run Method for Comprehensive and Simultaneous Analysis of Polar Metabolome One of the technical challenges in the field of metabolomics is the development of a single-run method to detect the full complement of polar metabolites in biological samples. However, an ideal method to meet this demand has not yet been developed. Herein, we proposed a simple methodology that enables the comprehensive and simultaneous analysis of polar metabolites using unified-hydrophilic-interaction/anion-exchange liquid chromatography mass spectrometry (unified-HILIC/AEX/MS) with a polymer-based mixed amines column composed of methacrylate-based polymer particles with primary, secondary, tertiary, and quaternary amines as functional groups. The optimized unified-HILIC/AEX/MS method is composed of two consecutive chromatographic separations, HILIC-dominant separation for cationic, uncharged, and zwitterionic polar metabolites [retention times (RTs) = 0–12.8 min] and AEX-dominant separation for polar anionic metabolites (RTs = 12.8–26.5 min), by varying the ratio of acetonitrile to 40 mM ammonium bicarbonate solution (pH 9.8). A total of 400 polar metabolites were analyzed simultaneously through a combination of highly efficient separation using unified-HILIC/AEX and remarkably sensitive detection using multiple reaction monitoring-based triple quadrupole mass spectrometry (unified-HILIC/AEX/MS/MS). A nontargeted metabolomic approach using unified-HILIC/AEX high-resolution mass spectrometry (unified-HILIC/AEX/HRMS) also provided more comprehensive information on polar metabolites (3242 metabolic features) in HeLa cell extracts than the conventional HILIC/HRMS method (2068 metabolic features). Our established unified-HILIC/AEX/MS/MS and unified-HILIC/AEX/HRMS methods have several advantages over conventional techniques, including polar metabolome coverage, throughput, and accurate quantitative performance, and represent potentially useful tools for in-depth studies on metabolism and biomarker discovery.

Microflow LC meets multinozzle ESI: A powerful setup enabling enhanced lipidomics coverage with 15x less sample. Great read by Meister et al. in *Analytical Chemistry*!

#lipidomics #massspec #microflow

🔗 pubs.acs.org/doi/10.1021/...

Hyphenation of microflow chromatography with electrospray ionization mass spectrometry for bioanalytical applications focusing on low molecular weight compounds: A tutorial review Benefits of miniaturized chromatography with various detection modes, such as increased sensitivity, chromatographic efficiency, and speed, were recognized nearly 50 years ago. Over the past two deca...

Also check out our review about the fundamentals of #microflow #chromatography, lots of tips and tricks
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...

Top ion map is classical RP lipidomics, bottom is using microflow-multinozzle ESI MS. Sterol esters ionize markedly better

We combined #microflow with #multinozzle ionization-HRMS for #untargeted #lipidomics
Look at CEs!