Recent #Research by Stephanie F. Gaudreau & Tuan V. Bui from @uottawa.ca
examines the distinct #developmental dynamics of opposing persistent currents shape #motoneuron firing during motor maturation of zebrafish 🐟️ 🧠
📜 Read the study: physoc.onlinelibrary.wiley.com/doi/10.1113/...
Top: Schematic representation of the experimental protocol. For each training (1 to 4), trained mice realized five successive swimming sessions (S1 to S5) of 15 seconds separated by 45 second breaks twice a day (9 AM and 5 pm) on post-natal day 1 (P1) and P2. Untrained mice were tested only once either at P1 9 AM or P1 5 pm or P2 9 AM or P2 5 pm during two swimming sessions of 7 seconds each (S1 green box and S2 red box). The greatest number of limbs used during the first 7 seconds of each session was scored (motor score). Bottom left: representative confocal images of transverse tibialis anterior section from a P3 mouse, showing neurofilament (NF), α-bungatoxin (α-BTX,) and laminin labeling. Bottom right: representative image of NMJ detection (upper panel) and 3D reconstruction (lower panel) with Fiji. Calibration bars: 10 μm.
The neuromuscular system is highly plastic in early life. This study shows that brief #swim training in mouse pups accelerated swimming pattern acquisition & altered #motoneuron properties, revealing the sensitivity of the #neuromuscular system during development @plosbiology.org 🧪 plos.io/3S3eRdp
Top: Schematic representation of the experimental protocol. For each training (1 to 4), trained mice realized five successive swimming sessions (S1 to S5) of 15 seconds separated by 45 second breaks twice a day (9 AM and 5 pm) on post-natal day 1 (P1) and P2. Untrained mice were tested only once either at P1 9 AM or P1 5 pm or P2 9 AM or P2 5 pm during two swimming sessions of 7 seconds each (S1 green box and S2 red box). The greatest number of limbs used during the first 7 seconds of each session was scored (motor score). Bottom left: representative confocal images of transverse tibialis anterior section from a P3 mouse, showing neurofilament (NF), α-bungatoxin (α-BTX,) and laminin labeling. Bottom right: representative image of NMJ detection (upper panel) and 3D reconstruction (lower panel) with Fiji. Calibration bars: 10 μm.
The neuromuscular system is highly plastic in early life. This study shows that brief #swim training in mouse pups accelerated swimming pattern acquisition & altered #motoneuron properties, revealing the sensitivity of the #neuromuscular system during development @plosbiology.org 🧪 plos.io/3S3eRdp
Top: Schematic representation of the experimental protocol. For each training (1 to 4), trained mice realized five successive swimming sessions (S1 to S5) of 15 seconds separated by 45 second breaks twice a day (9 AM and 5 pm) on post-natal day 1 (P1) and P2. Untrained mice were tested only once either at P1 9 AM or P1 5 pm or P2 9 AM or P2 5 pm during two swimming sessions of 7 seconds each (S1 green box and S2 red box). The greatest number of limbs used during the first 7 seconds of each session was scored (motor score). Bottom left: representative confocal images of transverse tibialis anterior section from a P3 mouse, showing neurofilament (NF), α-bungatoxin (α-BTX,) and laminin labeling. Bottom right: representative image of NMJ detection (upper panel) and 3D reconstruction (lower panel) with Fiji. Calibration bars: 10 μm.
The neuromuscular system is highly plastic in early life. This study shows that brief #swim training in mouse pups accelerated swimming pattern acquisition & altered #motoneuron properties, revealing the sensitivity of the #neuromuscular system during development @plosbiology.org 🧪 plos.io/3S3eRdp
