New original research #paper alert 🚨 by Sian et al.: Optimizing 2D in vitro #differentiation conditions for #C2C12 murine #myoblasts on gelatin #hydrogel

#myoblue

doi.org/10.1007/s109...

Fig. 1. Automated continuous chaotic bioprinting. a) Schematic illustration showing automated chaotic bioprinting of noodles with internally aligned lamellar microstructures, using the three-element KSM (Kenics static mixer) printhead, with highlighted lateral and transverse views. b) Schematics of various three-dimensional (3D) hybrid food items, produced by simultaneous chaotic bioprinting of microalgae and muscle cells. c) Schematic illustration showing bioink designs for microalga and muscle cell-bioprinting.

Fig. 6. 3D-bioprinted hybrid food. a) Photographs showing 3D hybrid drumsticks on day 0 and day 16, bioprinted with C2C12 cell- and Chlamydomonas cell-bioinks. b) Viability values of bioprinted C2C12 cells and Chlamydomonas cells within the hybrid drumsticks, assessed over the 15-day culture period at 37 °C. Data are presented as themean values ± SEMs (n = 3 independent samples, each obtained from a separate bioprinting run). c) Fluorescence confocal micrographs showing the expression of skeletal muscle myosin (green) by C2C12 cells and Chlamydomonas cells with red autofluorescence of chlorophyll on day 16, within bioprinted hybrid drumsticks. d) Photographs of bioprinted hybrid cuboids at day 0 and day 14, using C2C12 cell- and Chlamydomonas cell-bioinks. e) Photographs of cut pieces of bioprinted hybrid cuboids on day 21, used for cooking studies. f) Comparison of mechanical properties of hybrid food before and after cooking on day 21. Data are presented as the mean values ± SEMs (n = 4 independent samples, each obtained from a separate bioprinting run) and a two-tailed paired t-test was applied to measure p values.

Fig. 7. Chaotic-bioprinted chicken-microalga hybrid noodle. a) Photograph of hybrid chicken-microalga noodles on day 21, bioprinted with chicken cell- and Chlamydomonas cell-laden bioinks. b) Fluorescence micrograph showing live chicken cells (green) and Chlamydomonas cells with red autofluorescence of chlorophyll, within the bioprinted hybrid chicken-microalga noodles on day 21. c) Viability values of chicken cells and Chlamydomonas cells within bioprinted hybrid chicken-microalga noodles, assessed over the 21-day culture period at 37 °C. Data are presented as the mean values ± SEMs (n = 3 independent samples, each obtained from a separate bioprinting run). d) Fluorescence confocal micrographs showing the expressions of F-actin, MYH2, and skeletal muscle myosin (green) by chicken cells and Chlamydomonas cells with red autofluorescence of chlorophyll, within bioprinted chicken-microalga hybrid noodles on day 21.

Interesting work by Maharjan et al. (2025) on the 3D #bioprinting of plant- and animal cell-based hybrid foods, especially noodles that are made of 30–40% edible #microalgae ( #Chlamydomonas or Chlorella) and 60–70% muscle cells (C2C12 or chicken #myoblasts) 🍜🍗🧐.
🔗 www.nature.com/articles/s41...