Close-up view of maize roots growing in soil within a rhizobox. Three roots are within clear plastic tubes with liquid and have an associated post-it note with details of the treatment next to the tubes. Photo credit: Erin Sparks

Evaluating nitrogen uptake: Methods and applications for plant research

A new #AppsPlantSci review by Pierce, @razda5.bsky.social, Huddell & @erinsparks.bsky.social

bsapubs.onlinelibrary.wiley.com/doi/full/10.... @danforthcenter.bsky.social #botany #plantscience #nitrogenfertilization #nanoSIMS

We used #isotope incubations, #nanoSIMS, fluorescence microscopy and 'omics to tackle how urea is used for nitrification, and by which organisms, in the coastal Gulf of Mexico, the open ocean Angola gyre, and the Black sea.

Huge congrats to everyone involved! 🦠

Visiting the #nanoSIMS facility @openuniversity.bsky.social

Our colleague Angela Vogts and key collaborator for @erc-expand.bsky.social presenting at the #nanoSIMS international workshop @noc.ac.uk @ird-fr.bsky.social

Getting ready for the #nanoSIMS workshop @openuniversity.bsky.social next week

GitHub - lpolerecky/LANS: A MATLAB-based program for processing and analysis of nanoSIMS data A MATLAB-based program for processing and analysis of nanoSIMS data - lpolerecky/LANS

For our #nanoSIMS data processing we use Look@nanoSIMS software, or LANS for short:
geolab.sites.uu.nl/looknanosims...

There has been an extensive update in the manual, so this is the time to check it out if you havent already! find it here:

github.com/lpolerecky/L...

Doing my last edits on my keynote for today - bringing some #soilscience into the physics realm #SIMSEurope #SoilBiogeochemistry #NanoSIMS @alixvidal.bsky.social @tuberlin.bsky.social @ucph.bsky.social

Comparing Raman and NanoSIMS for heavy water labeling of single cells | Microbiology Spectrum Accurate and reliable measurements of cellular properties are fundamental to understand the function and activity of microbes. This study addresses to what extent Raman microspectroscopy and nano-scal...

New paper out! In the @environmicrobio.bsky.social lab, we measured deuterium incorporation in 543 single cells using Raman and NanoSIMS. This allowed us to directly compare each technique and resolve any possible methodological artifacts
#NanoSIMS #Raman

journals.asm.org/doi/10.1128/...

A multimodal imaging pipeline to decipher cell-specific metabolic functions and tissue microenvironment dynamics Nature Protocols - Evaluating the metabolic organization of complex tissue microenvironments is useful for understanding cellular function within tissues. This protocol describes a multimodal...

I'm happy to share my latest work published in #NatureProtocols.

Read it here:

For those without a subscription - rdcu.be/d7WGO

For those with a subscription - www.nature.com/articles/s41...

#imaging #microscopy #cancer #immunology #Tcells #Bcells #SEM #NanoSIMS
#FrancisCrickInstitute #CRUK

Time to play "What's that #NanoSIMS component?"

SURFconext

Great #sclero study by Cornélia Brosset and colleagues describing using #nanoSIMS measurements to precisely separate #day and #night layers in #giantclams to study the relationship between #shell #chemistry and water #temperature.
www.sciencedirect.com/science/arti...

NanoSIMS mass spec images of Brady soils amended with 13C root detritus, showing associations between mineral particles,aggregates, and fungal hyphae

As part of her thesis work, Abbygail R. McMurtry added 13C enriched roots to paleosols from Nebraska and tracked their fate in mineral-associated pools
w/ Marie-Anne de Graaff @emsaurios.bsky.social @aaberhe.com he.com @cwmsoils.bsky.social @moreorloess.bsky.social
#NSF #nanoSIMS #LLNL #doescience

NanoSIMS mass spectrometer and florescence images illustrating how cells of F. sacchari from sediments of Great Boiling Spring have assimilated 13C- starch and 15N-ammonium

#NanoSIMS evidence of 13C- starch and 15N-ammonium assimilation

Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota Nature Communications - Few aerobic hyperthermophilic microorganisms are known to degrade polysaccharides. Here, Nou et al. use genomic information to enrich and optical tweezers to isolate an...

New aerobic polysaccharide-degrading isolate from #GreatBoilingSpring
Genome-guided isolation of hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota
rdcu.be/d1gbR
w/ B. Hedlund, M Palmer, J Dodsworth, A Dekas, #LLNL #nanoSIMS

The facilities at University De Pau Et Des Pays De L'adour are AMAZING! Excellent #ICPMS, multicollectors, femtosecond laser, #NanoSIMS #TOFSIMS. Plus we got to wear very fetching hair nets and to see matthias vignon with a beard net 🤣 #otolith #isotope