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Green sprouts

Green sprouts

And there they are, future peas to harvest. Now they will be transfered to the greenhouse for more light & less warmth.
#allotment #pisum #growyourgreens

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Erwt Akelei, een sugarsnap

Erwt Akelei, een sugarsnap

Op de vensterbank

Op de vensterbank

My way of circumventing the mice in the garden: sowing in flat containers divided by cardboard. In this way I get little rows that I can plant out later this month. Until the greens peep out they are in the kitchen, later they move to the greenhouse 🌱
#tuinieren #pisum #erwten

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Soaking dry seeds

Soaking dry seeds

Next step soaking the pea seeds so I can sow them tomorrow. Every year I choose a blue pod variety because it makes harvesting easy.

#allotment #permaculture #pisum
#sowing

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Fig. 8 Hypothetical model showing the reciprocal regulation of target of rapamycin (TOR) and abscisic acid (ABA) signaling during the transition from the cell division to the cell expansion phase of legume seed development.
The activators of TOR observed in this paper are shown, but further molecular and hormonal influences on the developmental transition are omitted for simplicity.
The bottom panel extends a basic model by Weber et al. (2005) to show the approximate timing of relative changes in three established signaling metabolites: Glc, glucose; Suc, sucrose; and Gln, glutamine. Sucrose levels are known to be correlated with the signaling metabolite T6P.

Fig. 8 Hypothetical model showing the reciprocal regulation of target of rapamycin (TOR) and abscisic acid (ABA) signaling during the transition from the cell division to the cell expansion phase of legume seed development. The activators of TOR observed in this paper are shown, but further molecular and hormonal influences on the developmental transition are omitted for simplicity. The bottom panel extends a basic model by Weber et al. (2005) to show the approximate timing of relative changes in three established signaling metabolites: Glc, glucose; Suc, sucrose; and Gln, glutamine. Sucrose levels are known to be correlated with the signaling metabolite T6P.

Fig. 5. Glutamine (Gln) activates target of rapamycin (TOR) in developing pea cotyledons.
(a) The in vivo RPS6-Ser240 phosphorylation status in pea embryos throughout development. Replicate immunoblots are shown in Supporting Information Fig. S5.
(b) The relative RPS6-Ser240 phosphorylation signal is plotted, and the fresh weight of the embryos is plotted against days after pollination (DAP). Error bars represent the standard error of the mean.
(c–e) RPS6-Ser240 phosphorylation was assessed in 90–130 mg pea embryos incubated for 2 or 4 h with or without Gln (62.5 mM) and sucrose (142 mM) (c), Gln (62.5 mM) and glucose (100 mM) (d) or individual amino acids (62.5 mM) and NH4NO3 (20 mM) (e). 
Two biological replicates are shown. Replicate immunoblots are shown in Fig. S6.

Fig. 5. Glutamine (Gln) activates target of rapamycin (TOR) in developing pea cotyledons. (a) The in vivo RPS6-Ser240 phosphorylation status in pea embryos throughout development. Replicate immunoblots are shown in Supporting Information Fig. S5. (b) The relative RPS6-Ser240 phosphorylation signal is plotted, and the fresh weight of the embryos is plotted against days after pollination (DAP). Error bars represent the standard error of the mean. (c–e) RPS6-Ser240 phosphorylation was assessed in 90–130 mg pea embryos incubated for 2 or 4 h with or without Gln (62.5 mM) and sucrose (142 mM) (c), Gln (62.5 mM) and glucose (100 mM) (d) or individual amino acids (62.5 mM) and NH4NO3 (20 mM) (e). Two biological replicates are shown. Replicate immunoblots are shown in Fig. S6.

Great work by O’Leary et al. (2025) on how Gln-driven #PlantTOR signaling occurs during the early development of pea embryos (i.e. cell division phase), but is unexpectedly inactive during #legume seed storage protein biosynthesis. #Pisum #glutamine

🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...

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65886.01 Pisum sativum 'Sugar Magnolia' #PisumsativumSugarMagnolia #Pisumsativum #Pisum #pea #vegetable #seed #food #brain #peabrain

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#GenéticaMendeliana en mi jardín 🤓🫛
#science
#genes
#genetics
#Pisum

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