#ultraID

Benchmarking of proximity-dependent biotinylation enzymes across cellular compartments and time windows Proximity-dependent biotinylation has become a powerful approach for mapping protein interactions and subcellular organization in living cells. Although a growing number of engineered biotin ligases h...

www.biorxiv.org/content/10.1...
For anybody doing proximity-dependent biotinylation have a look at this reference preprint from the @gingraslab.bsky.social . Ten enzymes benchmarked in various conditions, it appears #ultraID is the enzyme of choice for combined specificity, speed and low background🧪