Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis - Nature Metabolism In vivo regulation of neuronal lipid droplet formation mediates whole-body energy homeostasis in a sex-specific manner in Drosophila and mice.

Beyond delighted to share our amazing collaboration with Thierry Alquier's lab @alquierthierry.bsky.social on a conserved role for neuronal lipid droplets in regulating energy homeostasis in vivo led by Celena Cherian, Romane Manceau, Danie Majeur, and Colin Miller www.nature.com/articles/s42... /1

The reticulon homology domain of Pex30 generates membrane curvature at ER subdomains for lipid droplet biogenesis www.biorxiv.org/content/10.6...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Stunning, Andre!! Great to see this out.

It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that:
www.nature.com/articles/s41...

Visualizing suborganellar lipid distribution using correlative light and electron microscopy - Nature Cell Biology Lennartz et al. introduce a correlative light and electron microscopy workflow, Lipid-CLEM, combining near-native lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively a...

I am extremely excited that my first first-author paper from my PhD is finally out 😆!! We developed a new CLEM workflow to measure lipid densities at the nanoscale. So if you are into lipids and super-resolution imaging approaches, this one is for you: www.nature.com/articles/s41...

I’m so happy to join the Editorial Board of JCB! I look forward to handling your manuscripts

Glad to see our tools helping to advance the field!

PLIN5 phosphorylation orchestrates mitochondria lipid-droplet coupling to control hepatic lipid flux and steatosis - Nature Metabolism Using single-cell tissue imaging and spatial proteomics to study mitochondria–lipid droplet coupling in hepatocytes, PLIN5 phosphorylation is identified as a mediator of lipid flux and nutrient stress...

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🚨 New paper out in Nature Metabolism! Chapter 2 of our mitochondria zonation story (following our 2024 Nature Communications work). We asked how zonated liver mitochondria adapt when lipid flux surges. 🧵👇
www.nature.com/articles/s42...

Awesome paper and super useful resource of CRISPR-based screens to identify regulators of #LipidDroplet biology under different metabolic conditions: crisprlipid.org

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly - Nature CRISPR–Cas9 screening identifies CLCC1 as a factor that increases neutral lipid flux to prevent hepatic steatosis and promotes nuclear pore complex assembly by promoting membrane bending and fusi...

Sooo happy to share our new paper in @nature.com “CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.” A terrific collaboration with @arrudalab.bsky.social, led by co–first authors Alyssa Mathiowetz and Emily Maymand.
www.nature.com/articles/s41...

Wow, congratulations Prasanna!! So well deserved. 🥂🎉

Thrilled to share that the first part of my PhD work from @cohenlaboratory.bsky.social lab is now on bioRxiv! www.biorxiv.org/content/10.6... Check out the threads below:

Thanks Rachid! 🙏

Thank you, Bianca!

Thanks for your kind words, Andre!

Thank you, Toni!

bsky.app/profile/cohe...

I'm delighted to present TWO new preprints from my lab, led by @mczanellati.bsky.social and @sherryhsu.bsky.social, about how #organelles remodel throughout #NeuronalDifferentiation. Check out the threads about their papers below:

Thank you to all the co-authors – this was a wonderful collaboration with the lab of Mohanish Deshumukh. Thank you also to Wendy Salmon for expert microscopy advice, and to
NIGMS, @chanzuckerberg.bsky.social, and @allenphilanthro.bsky.social for funding! 13/13

A huge thank you to all co-authors, WendySalmon (UNC Hooker Imaging Core) for expert microscopy advice, and NIH
and @chanzuckerberg.bsky.social for funding!!

Our data support a model in which tubulin acetylation coordinates lysosome-ER interactions to facilitate lysosome remodeling. This work establishes tubulin acetylation as a key cytoskeletal regulator important for neuronal differentiation. 9/9

Finally, we asked whether the observed morphological changes were coupled to functional changes. Loss of acetylated microtubules leads to enlarged, highly acidified lysosomes, and accumulation of autolysosomes, consistent with defective lysosomal reformation. 8/9

We also looked at lysosome dynamics and maturation. We found tubulin acetylation governs lysosome dynamics by supporting normal fission frequency. 7/9

One of the top hits we found was altered lysosome-endoplasmic reticulum (ER) contact. Super-resolution microscopy reveals that lysosome-ER contacts preferentially associate with acetylated microtubules in iNeurons. 6/9

Leveraging our quantitative analysis pipeline github.com/SCohenLab/in..., we systematically profiled organelles in iNeurons. Loss of tubulin acetylation broadly alters organelle morphology, distribution, and interactions, with #lysosome-organelle interactions most affected. 5/9

Does tubulin acetylation dictate organelle behavior? We used quantitative multispectral imaging to simultaneously profile 8 membrane-bound organelles and decipher how tubulin acetylation shapes organelle architecture and interaction networks during neuronal differentiation. 4/9

Are these PTMs spatially patterned? We saw that they are spatially organized. Remarkably, a perinuclear population of acetylated tubulin progressively accumulates during neuronal differentiation. 3/9

Led by @sherryhsu.bsky.social, we began by profiling 3 tubulin post-translational modifications (PTMs) - acetylation, polyglutamylation, and detyrosination - during neuronal differentiation. In iPSC-derived neurons, we see dramatic enrichment of all 3 PTMs during differentiation. 2/9

During #NeuronalDifferentiation, #organelles dramatically reorganize. #Microtubules are essential tracks for transport. But are they just highways, or can they instruct organelle behavior? Are tubulin modifications a regulatory code that guides organelle position or function? 1/9