Happy our lab could contribute to this exciting story from the Thomas Becker and Nikolaus Pfanner labs! A protein #Translocase forms #LipidDroplet #Mitochondria #ContactSites via a substrate-mimicry mechanism! www.nature.com/articles/s41...

Awesome paper and super useful resource of CRISPR-based screens to identify regulators of #LipidDroplet biology under different metabolic conditions: crisprlipid.org

Such a pleasure to write this review on #LipidDroplet heterogeneity, dynamics and #organelle interactions with Mike @hennelab.bsky.social ! Thank you to the editorial team @natrevmcb.nature.com and to Yoni Malis for assistance with draft illustrations

Registration is open for the FASEB #LipidDroplet meeting in Scottsdale, July 26-30! This is THE premier North American conference on LDs, you do not want to miss it!

Supported by the DFG, FOR 5815 is an interdisciplinary research consortium dedicated to unveiling how #LipidDroplet (LD) heterogeneity contributes to fundamental cellular processes, health, and disease.

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.

☕ @mikelangelipid.bsky.social @olzmannlab.bsky.social & co identify a #LipidDroplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of #ferroptosis.
@berkeleymcb.bsky.social
bit.ly/49mTeyF

The Myo2 adaptor Ldm1 and its receptor Ldo16 mediate actin-dependent lipid droplet motility Zhao et al. identify the molecular machinery for lipid droplet motility, consisting of the type V myosin Myo2, the Myo2 adaptor protein Ldm1, and its surface receptor Ldo16. They uncover a molecular l...

Proud to share our study on #LipidDroplet #Motility #Mitochondria #MembraneContactSite now in Cell Reports @cp-cellreports.bsky.social! Big thanks to @rwedlichsoldner.bsky.social, @flofroehlich.bsky.social, @ruferbus.bsky.social, Daniel Kümmel lab, Thomas Becker lab! www.cell.com/cell-reports...

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis - Nature Cell Biology Lange et al. identify a lipid droplet quality control pathway in which FSP1 safeguards stored neutral lipids from lipid peroxidation, thereby preventing the induction of ferroptosis.

Excited to share our study out in @natcellbio.nature.com! Led by @mikelangelipid.bsky.social, we identify the first #LipidDroplet lipid quality control pathway: LD-localized FSP1 protects stored lipids from oxidative damage and prevents LD-initiated #ferroptosis.
www.nature.com/articles/s41...

This latest review explores the role of #Perilipin 2 (PLIN2) in #LipidDroplet biogenesis, contact sites, dynamics, and #Lipophagy, spotlighting new #therapeutic opportunities targeting #PLIN2 for #LiverDisease intervention. #medsky

#OpenAccess: www.sciencedirect.com/science/arti...

Spotlight @jcb.org: Zhang and Ding @kumedcenter.bsky.social highlight new work from Sen et al. (rupress.org/jcb/article/...), describing how ethanol impairs hepatocyte #LipidDroplet breakdown via a novel UBXD8-p97/VCP-HSD17β13 axis. rupress.org/jcb/article/...

#Steatosis

Spotlight: Chen Zhang and Wen-Xing Ding @kumedcenter.bsky.social highlight new work from Sen et al. (rupress.org/jcb/article/...), describing how ethanol impairs hepatocyte #LipidDroplet breakdown via a novel UBXD8-p97/VCP-HSD17β13 axis. rupress.org/jcb/article/...

#Steatosis

In @jcb.org, Sen, Weller, McNiven et al. found the association of a #LipidDroplet protein segregase ATPase, VCP/p97, is reduced in EtOH-damaged hepatocytes, preventing the targeting of a key LD enzyme HSD17β13 to the lysosome, thereby increasing LD content in the cell rupress.org/jcb/article/...

Sen, Weller, McNiven et al @mayoclinic.org found the association of a #LipidDroplet protein segregase ATPase, VCP/p97, is reduced in EtOH-damaged hepatocytes, preventing the targeting of a key LD enzyme HSD17β13 to the lysosome, thereby increasing LD content in the cell rupress.org/jcb/article/...

Lipid Droplets: Cell-Biological Control of Metabolism Lipid droplets are master regulators of nutrient and energy homeostasis, but their functions go far beyond these classical roles: They are important in cellular differentiation and various stress res…

Learn about #LipidDroplet #biology, their role in human disease and more at EMBO Workshop "Lipid Droplets: Cell-Biological Control of #Metabolism" in Sant Feliu de Guíxols, Spain, 21–26 September 2025

Deadline: 10 June 2025

meetings.embo.org/event/25-lip...
#EMBOlipidDroplets #EMBOevents 🧪

I am beyond thrilled to co-organize this FASEB #LipidDroplet conference with Mike @hennelab.bsky.social in 2026! Check out the lineup and plan to participate - you do not want to miss this one!! #lipidtime

Dear #LipidDroplet friends, we are so much looking forward to the 2nd edition of our #EMBOLipidDroplets Workshop. Join us 21-26 September 2025 at beautiful Costa Brava!

Abstract submission deadline is 10 June 2025.

@embo.org
@biologists.bsky.social

meetings.embo.org/event/25-lip...

Triacylglycerol mobilization underpins mitochondrial stress recovery - Nature Cell Biology Baker et al. show that mitochondrial stress recovery requires mobilization of lipid droplet triacylglycerol stores to facilitate cardiolipin biosynthesis and mitochondrial biogenesis.

💫NEW: @zakbaker.bsky.social, Pagliarini & co show that #mitochondria stress recovery requires mobilization of #LipidDroplet triacylglycerol stores to facilitate #cardiolipin biosynthesis and mitochondrial biogenesis.
www.nature.com/articles/s41...

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis Lipid droplets (LDs) are organelles that store and supply lipids based on cellular needs. While mechanisms preventing oxidative damage to membrane phospholipids are established, the vulnerability of L...

Excited to share our new preprint! Exceptional work by @mikelangelipid.bsky.social identifies the first #LipidDroplet lipid quality control pathway! LD-localized FSP1 safeguards neutral lipids from peroxidation and prevents #ferroptosis. 🤩www.biorxiv.org/content/10.1101/2025.01....

Check out this preprint from collaborator Lance Johnson @johnsonlab.bsky.social and team characterizing the APOE3 vs E4 #LipidDroplet -ome! Some interesting Easter eggs in there 🐇🥚😉

Lipid Droplets: Cell-Biological Control of Metabolism Lipid droplets are master regulators of nutrient and energy homeostasis, but their functions go far beyond these classical roles: They are important in cellular differentiation and various stress res…

Dear #LipidDroplet friends, we just launched the website for the next #EMBOLipidDroplets Workshop in September 2025, again at beautiful Costa Brava. Check out the amazing lineup of speakers. Abstract submission deadline is 10 June 2025. Hope to see many of you there! #organelles #LipidMetabolism

Figure 2. ABHD1 over-expression results in LD formation and a higher TAG content. A. LD imaging in cells over-expressing ABHD1 in nitrogen-replete mixotrophic growth i.e. TAP medium (Three independent lines are shown, bar = 5 μm). B. TAG amount in WT versus ABHD1 overexpressing lines during nitrogen replete mixotrophic growth. Four independent experiments and for six independent lines (three technical replicates for each line) are shown. Error bars show mean and standard deviation, Mann-Whitney test: *** p = 0.0002. C. The distribution of LD numbers per cell during nitrogen replete mixotrophic growth (>200 cells examined per group). Histograms represent mean and standard deviation of three biological replicates for WT and five independent lines for OE (ABHD1 overexpressor). D. TAG quantification in the H1246 yeast strain expressing the Chlamydomonas ABHD1 gene. Yeast transformants expressing the respective gene were harvested and analysed for total TAG amount using GC-MS. Data are means of three biological replicates with standard deviation bars shown. A significance of q = 0.13 for EV vs. +CrABHD1, q = 0.02 for EV vs. +ScDGA1 and q = 0.13 for +ScDGA1 vs. +CrABHD1 were determined using the Kruskal–Wallis test followed by a two-stage step-up Benjamini, Krieger and Yekutieli false discovery rate procedure for multiple comparison correction.

Figure 6. A model explaining the multifaceted actions of lyso-DGTS and ABHD1 protein in promoting LD budding, growth and TAG synthesis. ABHD1 catalyzes the hydrolysis of lyso-DGTS to produce a free fatty acid and a GTS moiety. ABHD1 is present at basal levels during optimal growth, and it increases in transcription and protein amount as N starvation is initiated, paralleling the increases in both LD number and size [67]. During LD budding, the formation of lyso-DGTS and the synthesis of ABHD1 protein both promote positive membrane curvature therefore LD budding. Later on, ABHD1 activity may facilitate LD growth as less curvature is needed by degrading lyso-DGTS. The resulting FA can be activated to acyl-CoA by LCS2 and contribute to TAG synthesis. Additionally, by consuming lyso-DGTS, ABHD1 is pulling the flow of acyls from either a putative PDAT or a PLA2, whose activity drives either TAG or FA production, respectively. Indeed, in two knock-out mutants, LCS2 protein is present in reduced amount, likely because the flow of free FA is reduced without ABHD1. Taken together, the major role of ABHD1 lies in LD coat remodeling and maintain LD stability. Abbreviations: BTA1, betaine lipid synthase 1; DAG, diacylglycerol; DGAT, diacylglycerol acyltransferase; DGTS, diacylglyceryl-3-O-4'-(N,N,N-trimethyl)-homoserine; FAS, fatty acid synthase; G3P, glycerol-3-phosphate; GPAT, glycerol-3-phosphate acyltransferase; MLDP, major lipid droplet protein; LCS2, long chain acyl-CoA synthetase; Lyso-PA, lysophosphatidic acid; LPLAT, Lysophospholipid acyltransferase; PA, phosphatidic acid; PDAT, phospholipid:diacylglycerol acyltransferase; PLA2, phospholipase A2; TAG, triacylglycerol.

Great work by Torres-Romero, Légeret et al. (2024) on showing that the α/β hydrolase domain-containing protein 1 (ABHD1) has a lyso-DGTS lipase activity and its overexpression can promote #LipidDroplet formation and higher TAG content in #Chlamydomonas.
#Microalgae

academic.oup.com/nsr/advance-...

Attention #LipidDroplet friends. The website for our 2nd edition of the #EMBO Workshop about lipid droplets will be launched soon. Stay tuned for an amazing lineup of speakers and sign up here for automatic notification when we go online: coming-soon.embo.org/w25-16
#Organelles #LipidMetabolism

NMR #Metabolomics Conditioned medium of #EndothelialCell (HUVEC) #Senescence

⏬glucose consumption
⏬#Glycolysis
⏫Oxygen consumption
⏫#FattyAcidOxidation (LPL CD36 FABP5 CPT1A/B ACAD ACOX1 PPARγ #LipidDroplet)
⏫ATP production

#Antioxidants 2024
www.mdpi.com/2076-3921/12...

#DigitalHolographicMicroscopy
+#DeepLearning automated quantitation

Detects the optical thickness of #Nucleolus, (Huntingtin, TDP-43, ataxin-3) Aggregate, #StressGranule, #LipidDroplet in cultured cells

#Emboreports 2024
www.embopress.org/doi/full/10....

#Mechanobiology #SmoothMuscleCell

#Piezo1 & Pressure (hypertensive 180/120 mmHg) ▶️cPLA2-mediated #LipidDroplet formation & #FoamCell phenotype in SMC in 2 h🤠

SMC Epigenetics by 8 h Yoda1 (Piezo1 activator): LAD-heterochromatin, H3K9me3 Cut&Tag

#AdvSci 2023
onlinelibrary.wiley.com/doi/10.1002/...