Multi-Region Vascularized Brain Organoids
Integration of hiPSC-derived cerebral, mid/hindbrain & endothelial organoids

scRNAseq▶️"13 (!?🧐) previously uncharacterized endothelial-neural signaling interactions"

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Integrating Spatial Proteogenomics in Cancer Research Xx xx.

Integrating Spatial Proteogenomics in Cancer Research #AdvSci #MassSpec advanced.onlinelibrary.wiley.com/doi/10.1002/...

#Exosome from hTERT- #MesenchymalStemCell overexpressing PD-L1 + HGF i.v. Immediately following🐭Stroke
👉⏫Nestin+ neuroblast/astroglia
👉⏬Neural cell apoptosis
👉⏬Pro-inflamm leukocyte ⏫Anti-inflamm leukocyte
👉⏬infarct vol
⏫Neurobehaviors

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Spatial Multiomics▶️ aberrant activation of Polyamine Metabolism in Oral Submucous Fibrosis-derived Oral Squamous Cell Carcinoma

#SpatialTranscriptomics Visium
#SpatialMetabolomics #MassSpecImaging

High polyamine➡️⏬OSCC survival

#AdvSci 2024
advanced.onlinelibrary.wiley.com/doi/10.1002/...

👤Brain Organoids post-graft in 🐭prefrontal cortex & hippocampus

They gain regional traits😆

Prefrontal cortex niche (vs hippocampus)
➡️⏫TH+ dopaminergic & VACHT+ cholinergic features
➡️⏫Astrocyte branching & interactions with neurites

#AdvSci 2024
advanced.onlinelibrary.wiley.com/doi/10.1002/...

From Sample to Mixed Reality: A Translational 3D MALDI Imaging Platform for Advanced 3D Spatial Omics Analysis of 3D Cell Culture Disease Models A comprehensive technology platform enables high-fidelity, volumetric MALDI imaging of 3D cell cultures by integrating custom embedding molds, a semi-automated computational framework for 3D reconstr...

From Sample to Mixed Reality: A Translational 3D MALDI Imaging Platform for Advanced 3D Spatial Omics Analysis of 3D Cell Culture Disease Models #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

Microdroplet Stabilization Enabled Direct Mass Spectrometric Identification of Electrogenerated Transient Carbocation Intermediates The in situ mass spectrometric identification of the electrogenerated benzylic and phenylic carbocations, whose half-lives in bulk solutions are on the nanosecond scale, is achieved by taking advanta...

Microdroplet Stabilization Enabled Direct Mass Spectrometric Identification of Electrogenerated Transient Carbocation Intermediates #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

Image Fusion for Super‐Resolution Mass Spectrometry Imaging of Plant Tissue #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

“Time is brain” for cell therapies?🧠

Our #AdvSci perspective😆
@rrust.bsky.social
@dominikusbrian.com
@lydenlab.bsky.social

#StemCellTherapy for #Stroke should be timed to a biomarker-defined “window of receptivity”, not just the thrombolysis clock

advanced.onlinelibrary.wiley.com/doi/10.1002/...

Single‐Position Peptide Clustering for Peptidomics Reveals Novel Disease Biomarkers and Dysregulated Proteolytic Characteristics #AdvSci #MassSpec advanced.onlinelibrary.wiley.com/doi/10.1002/...

NRIP2+ #SmoothMuscleCell (likely mediating metabolic resilience by Leptin signaling) #AorticDissection

Leptin antagonist R128Q or AAV9-SM22a-NRIP2
⏬🐭AD #AorticRupture (BAPN)
⏫contractile SMC
⏫SMC mitochondrial ultrastructure & OXPHOS

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#Maraviroc CCR5i in fibrin hydrogel ⏫post-stroke survival & differentiation of hESC-cortical #NeuralPrecursorCell in🧠

At the ischemic core
👉⏫ ~10-fold survival (7 d)
👉NeuN+ PSD95+ neuron, laminin+ vascularization, ⏬CCL3/4/5 CCR5 (30 d)

#AdvSci 2023
advanced.onlinelibrary.wiley.com/doi/10.1002/...

INduced Pdgfrb+ #Pericyte ITGA8 KO🐭

Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#hiPSC-derived mononuclear phagocyte i.v. ▶️neurobehavioral benefits in Aged NSG or #AlzherimersDisease 5xFAD🐭

⏫Hippocampal Mossy cell
⏬Inflammation
↔️Aβ
Revert Transcriptomic Age of multiple neural cells

Injected cells do not home to🧠

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte

⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#DryAMD #AgerelatedMacularDegeneration

Rat focal #ChoroidAtropy◀️NaIO3 into suprachoroidal space
⏬Choroid thickness
⏬vessel density/junctions

partial rescued by #hiPSC-#EndothelialCell transplant 7 dpi
👉making 30~40% of choroid vessels

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#SpatialTranscriptomics #BrainOrganoid #Glioblastoma

Striatal implant of Brain organoids
+TERT p.C228T TP53 R248Q➡️SOX11+ Proneural GBM
+TERT p.C228T PTENΔ NF1Δ➡️ERBB3+ Mesenchymal GBM

Single-cell spatial transcriptional+CNV landscape

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#FGF9 ➡️Mechanosensitive Osteocyte-to-Osteoblast/Osteoclast signaling in Maxillary Bone formation

#CyclicStretch ➡️⏬FGF9 in MC3T3 preosteoblasts (😆)

FGF9⏫ in underdeveloped🧍‍♂️Maxilla

FGF9 ⏬osteogenesis &⏫osteoclastogenesis
ATF4/5/6 NR2F1

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Endothelin-1➡️ #EndothelialCell to #DorsalRootGanglion signaling in #VascularPain
✅Mechanical ❎Heat ❎Cold

1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍‍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Toward a Species Search Engine: KISSE Offers a Rigorous Statistical Framework for Bone Collagen Tandem Mass Spectrometry Data The Species Search Engine (KISSE) is a novel statistical approach for identifying species from collagen peptides, using a curated library of sequences and their relative abundances derived from shotg...

Toward a Species Search Engine: KISSE Offers a Rigorous Statistical Framework for Bone Collagen Tandem Mass Spectrometry Data #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

Hydrogen‐Deuterium Exchange Mass Spectrometry for Molecular Glue Characterization #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

A Mass Spectrometry Approach Reveals Fatty Acid Isomerism in Tomato Cold Tolerance #AdvSci advanced.onlinelibrary.wiley.com/doi/10.1002/...

#VascularCalcification

#Glutaminase1 (GLS1)-derived Glutamate drives #SmoothMuscleCell #Osteogenesis via NMDAR (▶️Ca2+/β-at), not AMPAR, mGlu

Both GLS1 & NMDAR ⏫in 👤calcified arteries #CKD

BPTES (GLS1i)⏬🐭arterial calcification (VitD3)

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Plasma Metabolic Profile with Machine Learning Reveals Distinct Diagnostic and Biological Signatures for Pathologic Myopia Plasma metabolic detection combined with machine learning offers a precise (area under the curve of 0.874) and high-throughput (<30 s) diagnosis for pathologic myopia (serious myopic macular degenera....

Plasma Metabolic Profile with Machine Learning Reveals Distinct Diagnostic and Biological Signatures for Pathologic Myopia #AdvSci #MassSpec advanced.onlinelibrary.wiley.com/doi/10.1002/...

DeepSecMS Advances DIA‐Based Selenoproteome Profiling Through Cys‐to‐Sec Proxy Training DeepSecMS, a deep learning platform leveraging a Cys-to-Sec proxy training strategy, enhances the identification of known selenoproteins and holds promise for discovering novel candidates. This proxy....

DeepSecMS Advances DIA‐Based Selenoproteome Profiling Through Cys‐to‐Sec Proxy Training #AdvSci #MassSpec ps://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202504109

Prediction of Lung Cancer Metastasis Risk Based on Single‐Cell Metabolic Profiling of Circulating Tumor Cells A self-developed CTC sorting and capture platform enabled single-cell metabolomics of patient/animal CTCs, revealing brain/bone metastasis-specific metabolic signatures. A classification model develo...

Prediction of Lung Cancer Metastasis Risk Based on Single‐Cell Metabolic Profiling of Circulating Tumor Cells #AdvSci #MassSpec advanced.onlinelibrary.wiley.com/doi/10.1002/...

ATF3 as Anti-Senescence transcription factor (with ATG7 as a direct target gene!) in #SmoothMuscleCell

AAV9-SM22α-shATF3⏫arterial #Senescence & Stiffness in SAMP8🐭

Terazosin, α1AR blocker
⏬YTHDF2-m6A ATF3 mRNA binding
⏬🐭Atherosclerosis

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Delayed Transplantation (7 vs 1 dpi) of #NeuralStemCell Improves Initial Graft Survival after #Stroke

⏫graft axon extension along #CorpusCallosum to contralateral hemisphere

@ctackenberg.bsky.social @rrust.bsky.social #AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

JAK2 #AorticAneurysm

#SmoothMuscleCell CCDC80 KO🐭
⏫Contractile-to-synthetic (prolif, inflam & MMP-hi) phenotypes by Angiotensin II
⏫ #AorticAneurysm Dissection

rescued by JAK2i WP1066

👤 #AorticDissection
⏬CCDC80 ⏫JAK2-STAT3 ⏫MMP2/9

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics Biosynthesis rates of 13 FFAs are quantitatively calculated in 15 tissues from glucose and an analytical framework to quantitatively calculate inter-tissue communications of fatty acids between the l...

Unraveling Tissue-Specific Fatty Acid Biosynthesis and Inter-Tissue Crosstalk in Mice through Stable-Isotope Tracing Metabolomics #AdvSci #MassSpec advanced.onlinelibrary.wiley.com/doi/10.1002/...