Riemannian Geometry, Optimal Transport & Spatial Multiomics Alignment 👹

Manifold Gromov-Wasserstein vs moscot SCOTv2 SpatialMeta

Visium + Xenium
Visium + #MassSpecImaging spatial metabolomics

Admit I have0️⃣understanding of the mathematical machinery😅

bioRxiv 2025
www.biorxiv.org/content/10.6...

Spatial Multiomics▶️ aberrant activation of Polyamine Metabolism in Oral Submucous Fibrosis-derived Oral Squamous Cell Carcinoma

#SpatialTranscriptomics Visium
#SpatialMetabolomics #MassSpecImaging

High polyamine➡️⏬OSCC survival

#AdvSci 2024
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#BranchedChainAminoAcid
Diabetic Atherosclerosis Calcification

#SpatialMetabolomics
#MassSpecImaging
Calcified👤tibial artery➡️
⏫BCAA metabolism

Propionyl-CoA, BCAA catabolic product▶️
H3K23 Propionylation
Smooth Muscle Cell Osteogenesis

SMC BCAT2 KO🐭

Research 2026
spj.science.org/doi/10.34133...

Excited to present our work at #EASLCongress 2025 (THU-036-YI). See you tomorrow at 1pm on the Track Hub 2 - Basic science to check how we use #massspecimaging and IMC for metabolic profiling of #liver fibrosis. Special thanks to our collaborators and funders @zoehall-icl.bsky.social

Using #massspecimaging we show #lipid #metabolism is dysregulated spatially in #liverfibrosis. By integrating MSI and IMC, we linked #sphingolipids with non-parenchymal cells. Targeting sphingolipid metabolic pathway in HSCs and hPCLSs showed anti-fibrotic effect.

MSIght: A Modular Platform for Improved Confidence in Global, Untargeted Mass Spectrometry Imaging Annotation Mass spectrometry imaging (MSI) has gained popularity in clinical analyses due to its high sensitivity, specificity, and throughput. However, global profiling experiments are often still restricted to LC-MS/MS analyses that lack spatial localization due to low-throughput methods for on-tissue peptide identification and confirmation. Additionally, the integration of parallel LC-MS/MS peptide confirmation, as well as histological stains for accurate mapping of identifications, presents a large bottleneck for data analysis, limiting throughput for untargeted profiling experiments. Here, we present a novel platform, termed MSIght, which automates the integration of these multiple modalities into an accessible and modular platform. Histological stains of tissue sections are coregistered to their respective MSI data sets to improve spatial localization and resolution of identified peptides. MS/MS peptide identifications via untargeted LC-MS/MS are used to confirm putative MSI identifications, thus generating MS images with greater confidence in a high-throughput, global manner. This platform has the potential to enable large-scale clinical cohorts to utilize MSI in the future for global proteomic profiling that uncovers novel biomarkers in a spatially resolved manner, thus widely expanding the utility of MSI in clinical discovery.

Excited that the software @hnmiles.bsky.social and I built for confident #MassSpecImaging has now been published! #JProteomeResearch @pubs.acs.org

✨ Fernando “Ralph” Tobias @ftobias.bsky.social – Secretary

✨ Boone Prentice @prenticelabuf.bsky.social – Counselor

✨ Jillian Johnson – Counselor

#IMSIS #MassSpecImaging #MSI #SpatialOmics #MassSpectrometry #ScienceLeadership #AnalyticalChemistry #TeamMassSpec

New year, new round of inspiring @eurobioimaging.bsky.social #VirtualPub talks.
We start this week with Cay Tressler on MALDI #MassSpecImaging. Join us on Friday at 1 pm CET - register below.

#SpatialProtomics
#MassSpecImaging
38 metal isotopes
#SystemicSclerosis Skin #Microangiopathy
Dynamic #EndothelialCell & #Pericyte subsets

Cell Neighborhood (25/40 µm) of EndMT->
T cell, Myofibroblast inner niche
Mast cell/Monocyte outer niche

#CircRes 2024
www.ahajournals.org/doi/10.1161/...