AngioTag
#Zebrafish kdrl+ #EndothelialCell RiboTag + TRAP-seq track endothelial translation activity in vivo

Discover novel EC functional genes😎

Profile Orangotypic endothelial features in🐟

Also UAS::RiboTag for any🐟tissues

Brant Weinstein lab #Angiogenesis 2025
link.springer.com/article/10.1...

#ThrowbackThursday in JHC: "Role of the Pancreatic Islet Microvasculature in Health and Disease."

Click to read for FREE: bit.ly/4fihNMw

#capillary #diabetes #endothelialcell

#EndothelialCell #MerTK #Efferocytosis #Atherosclerosis

EC MerTK KO🐭+ AAV8-PCSK9 + #HighFatDiet 2-mo
👉⏫lesion burden
👉⏫NADPH oxidase
👉⏫inflamm cytokines

Aortic Arch lesion #Proteomics➡️
⏫TGFβ HIF1α Wnt3a IL2
⏬LARP1 NAMPT TEAD1

Zufeng Ding lab #RedoxBiol 2025
www.sciencedirect.com/science/arti...

#TGF-β1 drives maladaptive #BoneMarrow endothelial repair—disrupting #VascularRegeneration and #hematopoietic support—while TGF-β1 inhibition restores #Endothelialcell function and promotes #multilineage recovery. @pku1898.bsky.social

#FeaturedArticle #STTT: doi.org/10.1038/s413...

Perturbing MEK–ERK–c-Myc bioenergetic axis in tumor #EndothelialCell suppress primary tumor growth

Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types

#PNAS 2025
www.pnas.org/doi/10.1073/...

GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell

GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain

Myh11+ SMC Gpr153 KO 🐭
Injured artery

Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke

#NatComm 2025
www.nature.com/articles/s41...

BBB on a chip

#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)

EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture

Does vascular basement membrane have such pores?🤔

#MatTodayBio
www.sciencedirect.com/science/arti...

INduced Pdgfrb+ #Pericyte ITGA8 KO🐭

Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#Atherosclerosis Epitranscriptomics

Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2

Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...

#THNO 2025
www.thno.org/v15p4785.htm

#WernerSyndrome hiPSC engineered vessel

Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell

#SpatialTranscriptomics
#Metabolomics

Vessels with Werner EC are Hypercontractile even with healthy SMC

bioRxiv 2025
www.biorxiv.org/content/10.1...

#TissueEngineeredBloodVessel

Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2

Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo

#CellStemCell 2025
www.sciencedirect.com/science/arti...

A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte

⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#Senescence #Mechanobiology

Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)

⏫F-actin, Calnexin ER, Lamin A Nuclear envelope

#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...

#Stroke #Angiogenesis

Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)

Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...

AAV-BI30, an endotheliotropic #AdenoAssociatedVirus for transducing vascular tree in #CentralNervousSystem Brain/Retina/Spinal Cord

👉>AAV-BR1 esp. in artery/vein

👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney

@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...

Stroke Atlas
anratherlab.shinyapps.io/strokevis/

Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)

9 #EndothelialCell clusters
IGF1 signals

#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)

#NatImmunol 2024
www.nature.com/articles/s41...

Single-cell transcriptomics #SuperficialTemporalArtery #MoyamoyaDisease

2 Case + 3 Ctrl (epilepsy)

SMC1: contractile OXPHOS
SMC2: ribosome

Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell

#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

#Malaria #BloodBrainBarrier

P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types

#NatCommun 2025
www.nature.com/articles/s41...

Endothelin-1➡️ #EndothelialCell to #DorsalRootGanglion signaling in #VascularPain
✅Mechanical ❎Heat ❎Cold

1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍‍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm

Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability

A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...

triggers inflammation throughout the body by activating immune & #endothelialcells.C5a also promotes the release of pro-inflammatory cytokines like IL-6.
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,

Genetic lineage tracing identifies intermediate mesoderm as a novel contributor to mammalian kidney lymphatics The lymphatic vasculature is essential for fluid homeostasis, immune regulation and possesses diverse organ-specific functions. During development, lymphatic endothelial cells (LEC) arise from multipl...

Lineage Origins of mouse renal #LymphaticEndothelialCell
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎

Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)

9 different Cre🐭🤠

@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...

Tie2+ #EndothelialCell- or Col1A2+ fibroblast-derived C-type Natriuretic Peptide protects against #AorticAneurysm in Apoe-/- mice + AngII + AAV-PCSK9 + #WesternDiet

#SmoothMuscleCell & Mφ are responsive to exogenous CNP

#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

Anti-isoDGR immunotherapy stabilizes Aged 🐭 #BloodBrainBarrier

#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging

isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence

bioRxiv 2025
www.biorxiv.org/content/10.1...

Serial block-face SEM of mouse Heart Capillaries #EndothelialCell #Pericyte #Telocyte

There are direct physical contacts b/w CD34+/CD44+ perivascular mesenchymal cells & abluminal protrusions of EC!

#Cells_MDPI 2025
www.mdpi.com/2073-4409/14...

Non-hepatic #PCSK9

PCSK9 overexpression regulates #ExtracellularVescle cargo packaging (Proteome microRNA array) in #SmoothMuscleCell▶️
👉⏫Inflammation in #EndothelialCell Mφ
👉⏫Migration ⏫oxLDL uptake ⏬OXPHOS ⏫Glycolysis in Mφ
👉Recruit more Mφ in🐟brain/muscle

#IJMS 2025
www.mdpi.com/1422-0067/23...

Pulsatile flow dynamics determine pulmonary arterial architecture BACKGROUND Single ventricle congenital heart disease (SV-CHD) is a uniformly lethal condition. Survival depends upon the Glenn surgery, which shunts venous blood directly to the pulmonary arteries wit...

Happy to share our work on the role of biomechanical PULSATILITY on lung vessels.

Following surgery to alleviate overload in kids with single ventricle hearts, lung arteries form AVMs. #DevBio #EndothelialCell #CHD

Beautiful work from Steve Spurgin. #DevBio 🫁 🫀 ❤️‍🩹

www.biorxiv.org/content/10.1...

"Plug"
Microglia (~20%) physically contract with #EndothelialCell & #Pericyte through gaps b/w adjacent #Astrocyte endfeet

A meticulous analysis of #MICrONS volumetric EM data
1 mm3 🐭visual cortex🤩

@gazz4science.bsky.social @sorengrubb.bsky.social #JCBFM 2024
journals.sagepub.com/doi/10.1177/...

AngioTag
Zebrafish kdrl+ #EndothelialCell RiboTag + TRAP-seq track endothelial translation activity in vivo

Discover novel EC functional genes😎

Profile Orangotypic endothelial features in🐟

Also UAS::RiboTag for any🐟tissues

Brant Weinstein lab #Angiogenesis 2025
link.springer.com/article/10.1...

#EndothelialCell GTPBP3, a mitochondrial enzyme in tRNA processing, ⏫#Angiogenesis in🐭embryo/retina/ischemic limb
Depending on mTORC1, not VEGFR/STAT/HIF1/NFκB

siGTPBP3▶️
⏫HRI▶️
⏫p-eIF2α/ATF4▶️
⏫Senstrin 2▶️
⏬mTORC1 activity
Also ⏫mitoROS ⏬OXPHOS

#Angiogenesis 2025
link.springer.com/article/10.1...