Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including a…

Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Mass spectrometry protocol for tear fluid proteome profiling and biomarker discovery Here, we present a protocol for tear fluid proteomics using Schirmer strips and liquid chromatography-tandem mass spectrometry (LC-MS/MS). It includes…

Mass spectrometry protocol for tear fluid proteome profiling and biomarker discovery #STARProtoc www.sciencedirect.com/science/arti...

Protocol for structural analysis of amyloid fibrils using hydrogen-deuterium exchange mass spectrometry Amyloid fibrils are common in many diseases. Here, we present a protocol that uses α-synuclein fibrils, a Parkinson’s disease hallmark, to demonstrate…

Protocol for structural analysis of amyloid fibrils using hydrogen-deuterium exchange mass spectrometry #STARProtoc www.sciencedirect.com/science/arti...

Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids Protein phosphorylation is an important post-transcription modification regulating essential cellular activities. Here, we present a proteomics protoc…

Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for the quantification of melatonin and AFMK in cerebrospinal fluid by LC-MS/MS Melatonin and its oxidant derivative, N-acetyl-N-formyl-5-methoxykynuramine (AFMK), are indolamines present in higher organisms, which are related to …

Protocol for the quantification of melatonin and AFMK in cerebrospinal fluid by LC-MS/MS #STARProtoc www.sciencedirect.com/science/arti...

Protocol for hybrid mass spectrometry-AI mapping of proteasome-generated HLA class I epitopes Proteasomes play a central role in antiviral and antitumor cytotoxic immunity, generating most of the >10,000 unique proteome-derived peptides bound t…

Protocol for hybrid mass spectrometry-AI mapping of proteasome-generated HLA class I epitopes #STARProtoc www.sciencedirect.com/science/arti...

Protocol to pinpoint oxidized and reduced cysteine residues in brown fat using differential alkylation labeling techniques Cysteine post-translational modifications are important for protein functions and protein-protein interactions. Here, we present a protocol to detect …

Protocol to pinpoint oxidized and reduced cysteine residues in brown fat using differential alkylation labeling techniques #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for RNA modification analysis by UHPLC-QqQ MS Over 170 RNA modifications have been reported across domains of life. Here, we present a protocol for identifying and quantifying modified nucleosides…

Protocol for RNA modification analysis by UHPLC-QqQ MS #STARProtoc www.sciencedirect.com/science/arti...

Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm

Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability

A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Lei…

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics Here, we present a protocol for spatial proteomic profiling of the tumor microenvironment in tonsil cancer using multiplexed imaging-powered deep visu…

Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS Acetylcholine (ACh) is a small-molecule neurotransmitter that regulates a wide range of neuronal and non-neuronal physiological functions. The non-neu…

Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS #STARProtoc www.sciencedirect.com/science/arti...

Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts Crabtree-positive yeasts rapidly consume glucose via glycolysis, making it difficult to experimentally estimate their actual glycolytic rate or flux. …

Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry Nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS) benefits untargeted metabolomics by enhancing sensitivity and integrating proteomics …

Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry #STARProtoc www.sciencedirect.com/science/arti...

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

Protocol for identification of proteins from deyolked zebrafish embryos #STARProtoc #MassSpec star-protocols.cell.com/protocols/4124

Protocol for label-free quantitative lysine lactylproteome profiling L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) deriv…

Protocol for label-free quantitative lysine lactylproteome profiling #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for spatial metabolomics and isotope tracing in the mouse brain Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. He…

Protocol for spatial metabolomics and isotope tracing in the mouse brain #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome Colostrum and milk from dairy sources consist of whey, casein, and fat, which have notable pharmacological properties due to their proteins and peptid…

Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for generating protein profiles and distance-based network analysis of murine tissue slices We introduce a protocol for spatial proteomics using thin cryotome sections of mouse skeletal muscle tissue. We describe steps for preparing muscle se…

Protocol for generating protein profiles and distance-based network analysis of murine tissue slices #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol to study secretome interactions using extracellular proximity labeling Biotin ligase-based proximity ligation is a widely used, highly effective technique for the study of in vivo protein-protein interactions. However, th…

Protocol to study secretome interactions using extracellular proximity labeling #STARProtoc #MassSpec www.sciencedirect.com/science/arti...

Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques Yeast serves as a functional alternative and sustainable protein source in aquaculture. This protocol outlines feeding strategy and intestinal proteom…

Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques #STARProtoc www.sciencedirect.com/science/arti...

Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics We present a low-volume, direct analysis protocol for non-targeted gas chromatography-mass spectrometry (GC-MS) metabolomics, using 100 μL of urine. T…

Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics #STARProtoc www.sciencedirect.com/science/arti...