Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm

Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability

A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...

Intraluminal pressure (100 Pa vs static) in a 3D #MicrovesselOnAChip (HUVEC φ200 μm collagen channel)➡️
⏫25%φ
⏫2.5-fold permeability (4kD FITC-dextran)
⏫EC stress fiber
👉Local nuclei clustering
👉Bimodal redistribution of junctional length

#iScience 2023
www.sciencedirect.com/science/arti...