VEGFA/VEGFR2/3➡️Rac1-RhoA/ROCK antagonism➡️⏫ #LymphaticEndothelialCell Barrier function in intestinal lacteal capillaries

A discontinuous button-like to continuous zipper-like junctional shift
⏫barrier to chylomicron uptake

#CircRes 2023 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

3D #LymphaticEndothelialCell Microvessel

Co-treatment with VEGF-A & VEGF-C, not individual factor➡️
1⃣Sprouting
2⃣Hyperpermeability
3⃣Collective realignment to longitudinal axis even in the absence of flow! ◀️tubular geometry-induced #CollectiveDurotaxis?🤓

bioRxiv 2025
www.biorxiv.org/content/10.1...

Genetic lineage tracing identifies intermediate mesoderm as a novel contributor to mammalian kidney lymphatics The lymphatic vasculature is essential for fluid homeostasis, immune regulation and possesses diverse organ-specific functions. During development, lymphatic endothelial cells (LEC) arise from multipl...

Lineage Origins of mouse renal #LymphaticEndothelialCell
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎

Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)

9 different Cre🐭🤠

@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...

scRNAseq of🐭collecting lymphatic vessels😎

Cell type-specific #LymphaticEndothelialCell vs peri-lymphatic myeloid cell contributions of TRPV4 in distinct stages of lymphatic contractile activity

@maryschulz.bsky.social
@jcastorena.bsky.social
#ATVB 2025
www.ahajournals.org/doi/full/10....

Heart #Lymphangiogenesis

LYVE1+ #LymphaticEndothelialCell KO🐭 of ACKR3, a decoy receptor for Adrenomedullin
post-Infarction
⏫survival
⏬Myocardial Edema
⏫peri-infarct #Lymphangiogenesis

siACKR3 #Hypoxia▶️
⏫LEC VE-cad junction

#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

Heart #Lymphangiogenesis

EphrinB2-mediated CDK5/ISL1 pathway
⏫🫀lymphangiogenesis
⏬ischemic injury by resolving post-MI inflammation

EphrinB2-CDK5-ISL1-VEGFR3
⏫ #LymphaticEndothelialCell proliferation migration➡️
Lymphatic clearance of Mφ

#SigTransductTargetTher 2025
www.nature.com/articles/s41...

🫀 #Lymphangiogenesis
#BranchedChainAminoAcid Catabolism
#HeartFailure

Prox1+ #LymphaticEndothelialCell BCKDK (kinase ⏬BCAA degradation) KO➡️
⏫Lymphangiogenesis
⏫Diastolic function
🐭HFpEF (L-NAME+HFD 12 wk)

Src-VEGFR3 axis

#CircAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/full/10....

2X #DilatedCardiomyopathy GWAS MATG #NatGenet 2024 reveal novel genes/cellular players

PROX1 - #LymphaticEndothelialCell
ERG - #EndothelialCell
DGKG - #SmoothMuscleCell
MAPT/Tau validated in both studies

>9k case
www.nature.com/articles/s41...

>14k case
www.nature.com/articles/s41...

Neural Guidance of Lymphatics

#SchwannCell CXCL12⏫ Sprouting/Patterning of CXCR4+ #LymphaticEndothelialCell

Prox1-CreERT2:Cxcr4f/f🐭
⬇️ Lymphatic Valve formation
⬇️ VEGF-C-induced #Lymphangiogenesis

Xiaolei Liu lab Development 2024 @biologists.bsky.social
journals.biologists.com/dev/article-...

Cardiac Lymphangiogenesis in CVDs

A concise review on #LymphaticEndothelialCell dynamics in #MyocardialInfarction #HeartFailure #Atherosclrosis

Lymphangiocrine▶️Reelin (cardiomyocyte), CCL2/CCL21 (macrophage)

#ATVB 2024
www.ahajournals.org/doi/10.1161/...