#Zebrafish🐟
#Pericyte

Progressive mural cell deficiencies across the lifespan in a foxf2 model of cerebral small vessel disease

#SciArt from Dr. Sarah Childs lab😍

#eLife 2026
elifesciences.org/articles/106...

Cleared wild-type 3-mpf 🐟brain
pdgfrβ (blue)
kdrl (red)

#Pericyte Preprint
#BloodLabyrinthBarrier

Single-cell Transcriptomic feature of Adult🐭Cochlear Stria Vascularis

CD31+NG2+ Pericyte Progenitor-like leader cells🤠 observed at the tip of vascular sprouts in 3D strial explant

#Rq 2026
www.researchsquare.com/article/rs-8...

Leukocyte stalling in 🐭Retinal microvasculature
2PM

In 20 s, "~7% tracked leukocytes stalled for at least one time along their path", up to 9 stalls

87% stalls occur at vessel junctions, where 71% occupied with #Pericyte

⏫RBC flow irregularity

#JCBFM 2025
journals.sagepub.com/doi/10.1177/...

⍺SMA- Collagen IV+ Interpericyte Tunneling Nanotubes in 👁️Macular vasculature decrease in #T2D diabetes patients (w/o retinopathy)
6 Case + 8 Ctrl

No overt change in Macular Capillary density, #Pericyte density or Pericytic TNT length/diameter

#Diabetologia 2025
link.springer.com/article/10.1...

Gelsolin as a selective #Pericyte marker vs #SmoothMuscleCell Fibroblast in mouse #SkeletalMuscle
Antibody R&D MAB8170 Cryosection

BulkRNAseq
(SkM PDGFRβ+SMA− pericyte vs PDGFRβ+SMA+ SMC) vs (Brain pericyte vs SMC)

#SciRep 2025
www.nature.com/articles/s41...

In 🐁, some #pericyte #PrimaryCilia penetrate #astrocytic #endfeet and reach the #neuropil - sometimes near #synapses! Could these #cilia act as sensory “antennae” 📡for #NeurovascularCoupling?
🧪🧵5/8

In 🐁🧠, #ultrastructure shows that #pericyte #cilia are often ensheathed by #astrocytic #endfeet. In 🚶‍♀️🧠, #astrocytes are swollen and cilia stay confined within the basal lamina. What are they sensing from the astrocytes?
🧪🧵4/8

Aggressive prostate cancer is associated with pericyte dysfunction

👉 buff.ly/hbBYh67

🖊️ @idibell_cat @ArkaitzLab

#ProstateCancer #PCSM #Pericyte

BBB on a chip

#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)

EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture

Does vascular basement membrane have such pores?🤔

#MatTodayBio
www.sciencedirect.com/science/arti...

INduced Pdgfrb+ #Pericyte ITGA8 KO🐭

Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte

⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#Pericyte #Retina

Pericyte Vegfa KO🐭
👉⏬Vascular radial outgrowth, sprouting tip cells P5
down P5
👉↔️Arterial patterning, fundus integrity, vascular permeability, OIR vessel obliteration/neovascularization, laser-induced CNV
👉Pericyte# ⬇️3 wk ⬆️6 mo

#Cells_MDPI 2025
www.mdpi.com/2073-4409/14...

Single-cell transcriptomics #OxygenInducedRetinopathy #Angiogenesis

3 #Pericyte subsets

Col1a1 shRNA intravitreal P7 (onset of hyperoxia)▶️
⏬OIR avascular & neovascular tuft-like, SM α-actin+ mural cell-covered area

Also ⏬mural cell coverage of laser CNV

#THNO 2023
www.thno.org/v13p2515.htm

CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitment Endothelial cell (EC)-pericyte interactions are known to remodel in response to hemodynamic forces, yet there is a lack of mechanistic understanding of the signaling pathways that underlie these event...

#Pericyte #Endothelium #Angiogenesis

Serial block-face SEM of mouse Heart Capillaries #EndothelialCell #Pericyte #Telocyte

There are direct physical contacts b/w CD34+/CD44+ perivascular mesenchymal cells & abluminal protrusions of EC!

#Cells_MDPI 2025
www.mdpi.com/2073-4409/14...

"Plug"
Microglia (~20%) physically contract with #EndothelialCell & #Pericyte through gaps b/w adjacent #Astrocyte endfeet

A meticulous analysis of #MICrONS volumetric EM data
1 mm3 🐭visual cortex🤩

@gazz4science.bsky.social @sorengrubb.bsky.social #JCBFM 2024
journals.sagepub.com/doi/10.1177/...

#GLP1R #Pericyte

GLP1R agonist (Exendin-4) relaxes cardiac #Pericyte & dilates constricted capillaries in oxygen-glucose-derived perfused🐭right ventricle wall
A role of KATP channel Kir6.1

bioRxiv 2025
www.biorxiv.org/content/10.1...

#Pericyte #Mechanobiology

Primary🐭cardiac pericyte (Ca2+) senses Compressive force (1 g/cm2 by calibrated weights on coverslip), via TRPM4 & TRPM7

TRPM4i, 9-phenanthrol, ⏬infarct size by 60% in🐭MI

@mishracbflab.bsky.social @physrep.bsky.social 2025
physoc.onlinelibrary.wiley.com/doi/10.14814...

New data that #pericyte contraction due to increased compression (pressure), such as occurs during acute #myocardial #infarction, depends on TRPM channels, #TRPM4 and #TRPM7. TRPM4 inhibition reduced infarct size in a mouse MI model.
doi.org/10.14814/phy...

PDGFRβ+ #Pericyte miR-15a/16-1 KO🐭MCAO➡️
⏫🧠reperfusion 3~28 dpi
⏬infarct size 7~28 dpi
⏫#Angiogenesis #Neurogenesis 28 dpi

miR-15a KD pericyte condition media⏫proliferation & migration of cultured🐭🧠microvascular ECs

#Angiogenesis 2025
link.springer.com/article/10.1...

Immune-Vascular Forebrain #Organoid #Assembloid to model APOE genotype (ε2/2 vs 3/3 vs 4/4) & #SARSCOV2 infection

Isogenic #hiPSC forebrain neural progenitor➡️neuron astrocyte oligodendrocyte

+hiPSC yolk sac➡️ #EndothelialCell #Pericyte #Microglia

bioRxiv 2025
www.biorxiv.org/content/10.1...

🫀 Heart failure with preserved ejection fraction accounts for half of #heartfailure cases. Here, Luxán and colleagues suggest reduction in STAT3 drives #pericyte loss, a hallmark of #HFpEF:

➡️ doi.org/10.1002/1873-3468.70057

Isolation methods and characterization of primary rat neurovascular cells - PubMed Neurovascular cells can be isolated through our optimized protocols that minimize cell loss and encourage the adhesion and proliferation of isolated cells. By identifying timepoints of viable glia and...

🧠 Publication the article is based on! For all your primary #astrocyte and brain microvascular #endothelial and #pericyte needs: pubmed.ncbi.nlm.nih.gov/38992711/

#Microfluidic #BloodBrainBarrier-On-A-Chip

Parallel microchannels 50x50 µm 0.15 dyn/cm2 0.5~1 Hz⬅️ #EndothelialCell
Polycarbonate membrane 8 µm pore
Milli-well⬅️ #Pericyte #Astrocyte
Pericyte Astrocyte⏫ microchannel EC coverage

#AdvHealthcMat 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

New issue online! www.embopress.org/toc/17574684...

This month's cover highlights the article by Bo He, Ruth Ganss & colleagues 👉 doi.org/10.1038/s443... 👉 #eribulin treatment normalizes tumor blood vessels by inducing #pericyte quiescence, thus reducing #hypoxia.

Scientific image by Bo He

#Flavivirus #BloodBrainBarrier

#WestNileVirus #UsutuVirus cross in vitro #BBB (🧠microvascular #EndothelialCell - ThinCert - #Pericyte #Astrocyte, 60 Ω/cm2)
w/o barrier disruption

BBB secretome
⏬MMP3 MCP1
⏫IFNβ CXCL10

@barryrockx.bsky.social x #NPJViruses 2025
www.nature.com/articles/s44...

Lung #Pericyte #Fibrosis

Tuj1/βIIITub+ Pericyte expands in #Bleomyin-induced mouse #PulmonaryFibrosis

A potential anti-fibrotic #Pericrine CXCL10

Not Collagen-depositing

Tubb3 KO🐭➡️exacerbated fibrosis

#SciArt
Yoh-suke Mukouyama lab bioRxiv 2025
www.biorxiv.org/content/10.1...

CD248 #Pericyte #Zebrafish🐟

cd248a (not cd248b) KO
⏬brain & trunk pdgfrb+ Pericytes (⏬proliferation ⏫hypoxia-induced apoptosis)
⏫#BBB permeability (Cadaverine 1kD)

@commsbio.nature.com 2025
www.nature.com/articles/s42...

"Authentic" #hiPSC-derived #Pericyte 😎

Sharing highly similar proteome/phosphoproteome to primary👤🧠cortical pericyte

Contractile

⏫ #BloodBrainBarrier & Neuroprotective (Pdgfrb F7/F7🐭)

Clear Aβ Tau (🧠slices 5xFAD🐭 APOE4 P3001S🐭)

@rrust.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...

#hiPSC derived #Pericyte

👉>80% Home to & >50% Arborize on Capillaries in live🧠slice of PdgfrbF7/F7🐭
in situ PDGFBB-PDGFRβ PLA assay😍

👉⏫Amyloidβ clearance on 10-m/o 5xFAD 🐭🧠slice
Are these phagocytic pericytes still vessel-bound?😁

@rrust.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...