Behold the microscopic realm full of wonders and mysteries #hiPSC #microscopy

Serna et al. used hiPSC-derived neurons and astrocytes, coupled with multielectrode array recordings, to characterize the electrophysiological activity of human brain cells under baseline conditions, following opioid exposure, and after naloxone treatment.

#neuroskyence #MEA #invitro #hiPSC

#TissueEngineeredVascularGraft

#hiPSC #SmoothMuscleCell on 3-mm PGA tubular scaffold

Decellularized

Rupture pres 1200 mmHg
Suture retent str 120 g

Re-endothelialized⏩rat aortic graft 30 d
❎thrombus
Repop by host EC

#CircRes 2022 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

#TissueEngineeredBloodVessel

Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2

Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo

#CellStemCell 2025
www.sciencedirect.com/science/arti...

Divergent TPM1 mutants in #HypertrophicCardiomyopathy (E62Q) vs #DilatedCardiomyopathy (E54K)

#hiPSC #Cardiomyocyte
Engineered heart tissue
Twitch kinetics
Diastolic stiffness
RNAseq/Proteomics

E62Q⬇️effective stiffness
E54K⬆️Tropomyosin–troponin I interaction

#JCI 2024
www.jci.org/articles/vie...

Screenshot from PDF of linked paper, showing the following information: Stem Cell Research; Lab Resource: Multiple Cell Lines. "Generation of human induced pluripotent stem cell lines from patients with PHACE syndrome.:"Rohin Ramchandania Chanatjit Cheawsamoot, Giovanni A. Torresa, Jennifer Arthur Ataama, Dawn Siegel, Ioannis Karakikesa. Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA, USA; Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Department of Dermatology, Stanford University School of Medicine, Stanford, CA, USA

Using blood samples from patients, researchers at @stanfordcvi.bsky.social developed a new #hiPSC line for studying PHACE syndrome and screening therapeutics.

Read the paper by Ramchandani et al. of @stanfordmedicine.bsky.social

www.sciencedirect.com/science/arti...

🧪 #Cardiosky #PHACE

Immune-Vascular Forebrain #Organoid #Assembloid to model APOE genotype (ε2/2 vs 3/3 vs 4/4) & #SARSCOV2 infection

Isogenic #hiPSC forebrain neural progenitor➡️neuron astrocyte oligodendrocyte

+hiPSC yolk sac➡️ #EndothelialCell #Pericyte #Microglia

bioRxiv 2025
www.biorxiv.org/content/10.1...

STAgent
#SpatialTranscriptomics AI Agent🤯

STARmap #hiPSC -pancreas graft

Multimodel LLM
Visual language reasoning
Dynamic code generation
Context-aware gene set analysis +Google Scholar literature
Final report assembly

Soon we yield all jobs to #AI🥹

bioRxiv 2025
www.biorxiv.org/content/10.1...

hiPSC on micropattern, in a heart shape.

🚨🚨 Paper alert : doi.org/10.1016/j.st...

Check out the work we did with Sarah Withey, Nicole Santucci, @nazsalehin.bsky.social , Bella Li and others in Patrick Tam's

We demonstrated the functional role of MIXL1 in human iPSCs, with some beautiful images. 🔬❤️ (1/7)

#stemcell #devbio #hiPSC

#hiPSC derived #Pericyte

👉>80% Home to & >50% Arborize on Capillaries in live🧠slice of PdgfrbF7/F7🐭
in situ PDGFBB-PDGFRβ PLA assay😍

👉⏫Amyloidβ clearance on 10-m/o 5xFAD 🐭🧠slice
Are these phagocytic pericytes still vessel-bound?😁

@rrust.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...

Redirecting

Great work from @zukailiu.bsky.social defining the first mesenchymal cells in human development as trophoblast-derived, and adding to the examples of evolutionary co-option of viral sequences contributing to our uniquely ape/human features. #hiPSC #MorPhiC #EvoDevo
doi.org/10.1016/j.ce...

🌉 Hello, San Francisco! Catch Vincent Truong at the Axion BioSystems World Tour on April 17! He’ll share how advanced hiPSC-based models are transforming #pain, #ALS & #CMT research.

🔗 Register now → axionbiosystems.com/axion-world-...

#AxionWorldTour #hiPSC #Neuroscience #NotYourAverageNeuron

Thrilled to share our latest research! Our manuscript on CHD1L’s role in neurodevelopment and its implications in 1q21.1 syndromes is now on BioRxiv! 🧬🐟🧠

🔬 Read our findings: doi.org/10.1101/2025...
#Neurodevelopment #1q21.1 #Zebrafish #hiPSC

@golzio-lab.bsky.social
@igbmc.bsky.social

Roses are red, violets are blue,
Old heart models lack the punch they once knew.
But on an Organ-Chip— #hiPSC so refined—
Flow and stretch bring true maturity in kind.

MEF2C (not MEF2A/B/D)⬆️ by 120-fold during #hiPSC #EndothelialCell differentiation (VEGF FGF2 BMP4)

shRNA MEF2C ▶️
No effects on hiPSC-EC differentiation or VCAM1 ICAM1 (by TNFα)
⏬in vitro angiogenesis

TMEM100 TRAIL as direct target genes of MEF2C

#StemCells 2023
academic.oup.com/stmcls/artic...

Shaking the flask or Vertical-Wheel Bioreactor of 3D #hiPSC spheroids▶️9-fold⏫#SmoothMuscleCell yield🤠

Therapeutic benefit of Intramuscular injection of hiPSC-SMC in NOD🐭#Limbischemia

Stem Cell Res Ther 2024
stemcellres.biomedcentral.com/articles/10....

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

How to fix a broken #heart 💔->❤️

#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.

Happy #Fluorescencefriday #BlueSky & #CardioSky!

@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬

Inflammed #hiPSC Blood Vessel #Organoid➡️drug screening for #Atherosclerosis

Spinning shear stress
eLDL/TNFα/IL1β
monocytic THP1 co-culture

THP1 cells squeeze along vascular lumen➡️Foam cell at mural cell layer

✅Nanosized #GrapheneOxide

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

🔥Super excited to drop our recent preprint🔥

biorxiv.org/cgi/content/...

Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️

#Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

Hey #BlueSky! Here is my first #FluorescenceFriday post!
This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬.

🔵 Cardiomyocytes
🔴 Endothelial cells
🟡 Fibroblasts

More coming next week! 🧪

#Science #Biology #CellBiology #SciArt
@benjaminbjohnson.bsky.social

Happy #FluorescenceFriday! ✨ Proud to show a 100x magnification of a #hiPSC derived #innerearorganoid, with a beautiful #haircell and its stereocilia pointing towards the lumen! 🔬👂

@otobiologyleiden.bsky.social

Confocal microscopy shows a mini 3D heart (Cardioid) as a sphere full of wavy orange, reds, and cyan in the process of colliding and merging with a blood vessel organoid filled with deep reds and dark blues.

Hello #BlueSky! Figured my first post had to be a #FluorescenceFriday 🧪 I work on #hiPSC cardiac disease modeling ❤️

Image shows the initial collision and fusion of a #Cardioid (right) with a blood vessel organoid (left) as part of a fun collaboration with @mvilacuenca.bsky.social!

628P Development of a CRISPR/CasX 4q telomeric region ablation strategy for FSHD1 using an isogenic hiPSC line and a FSHD1 fibroblast cell line Facioscapulohumeral muscular dystrophy type 1 (FSHD1), the second most common myopathy affecting adults, is linked to a contraction to less than 11 Re…

Euromuscular Disorders 43 (2024)

👉#FSHD Poster 628P 🇫🇷
Development of a #CRISPR/CasX 4q telomeric region ablation strategy for FSHD1 using an isogenic #hiPSC line and a FSHD1 fibroblast cell line

💡The D4Z4 locus ablation will be verified with the Nanopore technology.

#Microfluidic #BrainOnAChip

PDMS dual-channel, separated by 50-μm-thick porous membrane

#hiPSC -#EndothelialCell -Neuron + Primary #Astrocyte #Pericyte

Screening human TransferrinR1 antibodies-conjugated AAV9 vectors for #BBB crossing

#Pharmaceut_MDPI 2024
www.mdpi.com/1999-4923/16...

#hiPSC #Senorimotor #Organoid
->Neural/Microglia/Vessel/Striated myofiber
->Modeling the impacts of #AmyotrophicLateralSclerosis variants (TDP43/SOD1/PFN1) on #NeuroMuscularJunction

To study Pericyte-NMJ crosstalk?

Brian Wainger lab #NatureComms 2021
www.nature.com/articles/s41...