Organoids-On-a-Chip for Personalized Precision Medicine

3D printing of microfluidic chips
Three-jet in-air microfluidics
Human-on-a-chip

Drug screening/development on patient-specific Organoids-On-a-Chip

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/full/10....

Inflammed #hiPSC Blood Vessel #Organoid➡️drug screening for #Atherosclerosis

Spinning shear stress
eLDL/TNFα/IL1β
monocytic THP1 co-culture

THP1 cells squeeze along vascular lumen➡️Foam cell at mural cell layer

✅Nanosized #GrapheneOxide

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...

#PeripheralArteryDisease #Netrin

Exosome derived from Netrin 1-overexpressing 🐭#AdiposeDerivedStromalCell⏫
Reperfusion #Angiogenesis 🧐 #Arteriogenesis
Exercise capacity
M2 Mφ polarization
in Diabetic db/db🐭#LimbIschemia

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...

#ArteryOnAChip

Dual-channel flow
#EndothelialCell (1 Pa) + #SmoothMusclecell (0.0002 Pa)

Bulk RNAseq of EC-/+flow SMC▶️
Novel vascular regulatory genes in SMCs
CRYAB INHBA LRG1 ▶️
Validated in👤🐷🐭#Atherosclerosis #AbdominalAorticAneurysm

#AdvHealthcMater 2023
onlinelibrary.wiley.com/doi/10.1002/...

#SmoothMuscleCell #YAP/TAZ senses Matrix Topology (Flat, Cross-patterned, Line, Curved) (PMMA GPa; PDMS MPa)

2 segregatable Muscularization programs in SMCs？🤔
👉SM α-actin-based, ⏫by verteporfin (YAPi)
👉Calponin-based, ⏬by verteporfin

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...

#LungSealant with Alveoli-mimetic hydrofoam structure!😆

"gelatin (structural biopolymer), transglutaminase (crosslinking initiator), tannic acid (tissue adhesion promoter), and lung-derived matrikines (tissue repair promoter)"

#AdvHealthcMater 2024
onlinelibrary.wiley.com/doi/10.1002/...