From the latest issue:
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease
Multimodal non-invasive imaging of mouse #LimbIschemia
#LaserSpeckleImaging
#NearInfrared2ZoneImaging
#DuplexUltrasound Flow + Muscle elastography
#OpticalCoherenceTomographyAngiography
+Some human data #T2D
#BioengTranslMed 2025
aiche.onlinelibrary.wiley.com/doi/10.1002/...
📢 Online First!
Pasqui and colleagues share data to suggest that active malignancy increases mortality but does not affect limb salvage or reocclusion in patients with acute limb ischemia.
Read more 👉 journals.sagepub.com/doi/10.1177/...
#LimbIschemia #PeripheralArteryDisease #VascSky
#SGLT2i #Diabetes #PeripheralArteryDisease
Empagliflozin ⏫#Angiogenesis #Arteriogenesis in diabetic db/db🐭 #Limbischemia
A possible SETD2 (H3K36 methyltransferase)-antioxidant cascade
SETD2i EPZ-719 ⏬the benefits of EMPA in stressed HUVEC
#CardiovascToxicol 2025
www.gimopen.org/article/S294...
#Cuproptosis in #ChronicLimbThreateningIschemia
Bulk RNAseq re-analysis ➡️
12 Cuproptotic genes⏬in👤CLTI Gastroc muscle
Copper chelator Ammonium Tetrathiomolybdate▶️
⏬Muscle protein lipoylation
⏫Limb reperfusion
in diabetic🐭 #LimbIschemia
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
#PeripheralArteryDisease
#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia
Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)
#FreeRadicBiolMed 2025
www.sciencedirect.com/science/arti...
Crosslinked Alginate-Collagen #Hydrogel to stabilize & deliver #EndothelialCell (HUVEC)-derived #Extracellular Vesicle, encapsulated with platelet membrane to ischemic🐭limb
Lots of (which) cells express IL-1β in🐭ischemic muscle!😮
#LimbIschemia
#MacroJrnls_ACS 2024
pubs.acs.org/doi/10.1021/...
#CircadianRhythm #PeripheralArteryDisease
Perturbed #CircadianClock in #SkeletalMuscle (ACTA-rtTA-TRE-Cre::Bmall) impairs #Myogenesis (⏬myostatin, ⏫myofiber hypertrophy) & #Angiogenesis (Coupled by⏬VEGF-A) in🐭 #LimbIschemia
#ATVB 2024 @ahascience.bsky.social
www.ahajournals.org/doi/abs/10.1...
Bispecific antibody for #LimbIschemia
A dual-targeting antibody T11-RF12 for activating Tie2 & LGR5-FZD10 Wnt signaling in #EndothelialCells
Intramuscular injection (Gracilis) Balb/c🐭
⏫limb reperfusion
Rescue paw necrosis
⏫Angiogenesis Arteriogenesis
mAbs 2024
www.tandfonline.com/doi/10.1080/...
Mouse #LimbIschemia
Contrast-enhanced ultrasound (whole calf) vs Laser Doppler (primarily skin) imaging of limb reperfusion post-ischemia
C57BL/6J🐭♂️ 10~12 wk
B/w 2- & 4-wk post-ischemia
Laser doppler ⬆️
Ultrasound microbubble ⬇️
Ultrasound Med Biol 2024
www.sciencedirect.com/science/arti...
#ChronicLimbThreateningIschemia #LimbIschemia
Competing risk analysis to estimate #Amputation incidence & risk in #LowerExtremityPeripheralArteryDisease
7273 case + 5095 ctrl
2 yr f/up
journals.sagepub.com/doi/10.1177/...
#EndothelialCell #Mitochodria #LimbIschemia #EndMT
Thrilled to have played a small role in this exciting story😀
Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition
#ScienceAdv 2024
www.science.org/doi/10.1126/...
🐷bone marrow #MesenchymalStemCell encapsulated in Alginate #Hydrogel as cell therapy in 🐷model of #LimbIschemia Iliac arterial excision
Some MSCs survive up to 4 wk in vivo
Limb use - force-time impulses✅ trotting pattern✅
Hyperemic perfusion✅
#JAHA 2024
www.ahajournals.org/doi/10.1161/...
An important paper on Macrophage-#MuscleStemCell crosstalks in regenerative dynamics of ischemic #SkeletalMuscle #CriticalLimbThreateningIschemia
Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia
#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....
Condition Tie2+ Monocyte/Mφ to treat #LimbIschemia
siRNA HTATIP2⏫NRP1+Angpt1
Tie2+ Mφ from #ChronicLimbThreateningIschemia👤exhibits ⏬angiogenic+arteriogenic benefit in vitro & in🐭
Alberto Smith, Bijan Modarai labs #JCIInsight 2023
insight.jci.org/articles/vie...
MedSky🧪#IDSky CardioSky🫀 #CaseReport #COVID19 leads to a #hypercoagulable state that predisposes patients to #thromboembolism. Thrombotic events occur in the arterial circulation in 1-5% of cases & may cause #stroke, #acutecoronarysyndrome or #Limbischemia .
Mouse #LimbIschemia induced by #Photothrombosis
♂️ICR🐭
iv. Erythrosin B
Iliac artery, 100W cold white light, 30 min
vs artery ligation-ischemia
Saving surgical time
More severe ischemic myopathy
No reperfusion
Any embolic events?🧐
Microvasc Res 2019
www.sciencedirect.com/science/arti...
