#Cuproptosis in #ChronicLimbThreateningIschemia

Bulk RNAseq re-analysis ➡️
12 Cuproptotic genes⏬in👤CLTI Gastroc muscle

Copper chelator Ammonium Tetrathiomolybdate▶️
⏬Muscle protein lipoylation
⏫Limb reperfusion
in diabetic🐭 #LimbIschemia

#FreeRadicBiolMed 2025
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#PeripheralArteryDisease

#HyperbaricOxygenTherapy (100% O2, 3 ATA, 1 h/d for 5 d⬅️what's the control treatment?🤔) for mouse #LimbIschemia

Activate SIRT1➡️improve mitochondria fusion & OXPHOS in angiogenic #EndothelialCell (how?🧐)

#FreeRadicBiolMed 2025
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#SIRT7 protects against VitD3-induced🐭#VascularCalcification

SIRT7 does Not directly modify osteogenic factors RUNX2/BMP2 or contractile markers in 🐭aortic #SmoothMuscleCell +high Pi
Instead, SIRT7 appears antioxidant & anti-senescent

#FreeRadicBiolMed 2024
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