#SIRT6 #VascularCalcification

Rat #HighFatDiet + VitD3 + Nicotine
▶️Aortic medial calcification & ⏬SIRT6

SIRT6 overexpression ⏬Palmitic acid-induced #SmoothMuscleCell Apoptosis & Osteogenesis

#JHyperten 2023
journals.lww.com/jhypertensio...

#VascularCalcification
Resorbed or not?

When s.c. implanted in B6🐭, Calcium in Hydroxyapatite powder⏬
resorbed by TRAP-neg Multinucleated giant cells

In comparison
1⃣Calcified 👤tibial arteries 2⃣Pulverized🦴particles are resistant to calcium resorption

#ATVB 2025
www.ahajournals.org/doi/10.1161/...

#VascularCalcification

🐭aortic #SmoothMuscleCell in anchored 3D fibrin hydrogel for 14 d
👉Accumulate proteoglycan (IHC Proteomics)
👉No elastin fiber formation
👉TGFβ-responsive
👉Calcifiable, retaining similar individual sensitivity to 2D culture

www.sciencedirect.com/science/arti...

#LINE1 upregulation is identified as a driver of #VascularCalcification; silencing LINE1 or using #NRTIs suppresses cGAS‑STING signaling and mitigates VC—highlighting LINE1 inhibition and NRTIs as promising therapeutic strategies.

#OpenAccess in #STTT: doi.org/10.1038/s413...

DT-109 significantly reduces #atherosclerotic lesions and #VascularCalcification in #primates by downregulating #inflammation and #osteogenic markers, highlighting DT-109 as a promising therapy for #CardiovascularDisease.

#OpenAccess in #STTT: doi.org/10.1038/s413...

#VascularCalcification #SmoothMuscleCell m6A Epitranscriptomics

High Pi ⏬METTL3 in SMCs

METTL3-YTHDF2 destabilizes RUNX2 mRNA & ⏬ SMC Osteogenesis

S-Adenosylhomocysteine METTL3/14i aggravates aortic calcification in CKD 🐭 (adenine + hi Pi diet)

#CommsBio 2025
www.nature.com/articles/s42...

Diabetes-induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

➡️ buff.ly/bs13bDz

#Diabetes #VascularCalcification #Pyrophosphate

#VascularCalcification Mechanobiology

#HydrostaticPressure 120 mmHg 7 d➡️
⏫#Piezo1➡️ (⏫ #ERStress GPR78 CHOP➡️)
⏫ #SmoothMuscleCell Calcification (~20% level by 4 mM Pi)

#BBAMolBasisDis 2025
www.sciencedirect.com/science/arti...

#vascularCalcification

DNA N6-methyladenine/6mA (NOT m6A) of BMP2 gene ⏬OCT4-dependent transcription & #SmoothMuscleCell Calcification

ALKBH1, an eraser of 6mA
⬇️in calcified radial artery of👤CKD
⏫aortic calcification in 🐭+Adenine diet

@jci.org 2021
www.jci.org/articles/vie...

#LINE1 drives #VascularCalcification (VC) via cGAS-STING–mediated #inflammation, and silencing LINE1 or treating with #NRTIs suppresses VSMC osteogenic reprogramming, reduces VC in mice, and lowers VC incidence in humans. #medsky

#OpenAccess in #STTT: doi.org/10.1038/s413...

#VascularCalcification

#SmoothMuscleCell NAMPT-SIRT1 axis, via suppressing Notch-HES1 signaling, protects against aortic medial calcification in 🐭VitD3 or CKD

#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

That happy feeling when someone features a graphic you made in their talk 😇 #ASBMR2025 #vascularcalcification #ACDCmedical #MAC

Chronic Kidney Disease—Mineral and Bone Disorder (CKD-MBD), from Bench to Bedside

#OpenAccess
Full text 👉https://buff.ly/XY11LOV

#CKD
#KidneyHealth
#Dialysis
#BoneDisorder
#VascularCalcification
#Nephrology
#KidneyCare
#nephsky
#medsky

#VascularCalcification

#Glutaminase1 (GLS1)-derived Glutamate drives #SmoothMuscleCell #Osteogenesis via NMDAR (▶️Ca2+/β-at), not AMPAR, mGlu

Both GLS1 & NMDAR ⏫in 👤calcified arteries #CKD

BPTES (GLS1i)⏬🐭arterial calcification (VitD3)

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Gut alterations in a chronic kidney disease rat model with diet-induced vascular calcification

👉 buff.ly/ZkuYVrB

#ChronicKidneyDisease #VascularCalcification

#EndothelialCell to #SmoothMuscleCell communications in #VascularCalcification

CPP2, secondary calciprotein crystalline nanoaggregate, is internalized by EC(▶️Inflammation + altered secretome) & SMC (▶️calcification w/o osteogenic reprogram RUNX2 in 24 h)

#CMLS 2025
link.springer.com/article/10.1...

How does Genomic Instability cause #VascularCalcification?

A potential STING-STAT1-NLRP3 #Inflammasome cascade in #SmoothMuscleCell Osteogenesis #MedialArterialCacification

Which type of DNA lesions activates STING in calcifying SMC?🧐

#FASEBJ 2025
faseb.onlinelibrary.wiley.com/doi/10.1096/...

Endothelial-to-Mesenchymal Transition in Cardiovascular Pathophysiology
#EndMT

#Atherosclerosis
#VascularCalcification
#PeripheralArteryDisease
#PulmonaryHypertension
#ValvularHeartDisease
#MyocardialInfarction
#CardiacFibrosis

Krishna Singh lab #IJMS_MDPI 2024
www.mdpi.com/1422-0067/25...

DJ-1/PARK7 (causal gene of #ParkinsonDisease)⏬Diabetic #VascularCalcification

Acetylation (by NAT10)-Ubiquitination (by TRIM32) cascade degrades DJ-1▶️
⏫STAT1 & RAGE
⏫#SmoothMuscleCell Osteogenesis
⏫SMC MFGE8+ #MatrixVesicle▶️
⏫MV-collagen binding

#CardiovascRes 2024
academic.oup.com/cardiovascre...

#PCSK9 #VascularCalcification

50%⏫plasma PCSK9 in👤CKD+atherosclerotic calcification n=104 vs 330 Ctrl

300%⏫plasma PCSK9 in uremic rats
kidney as a PCSK9 source

#SmoothMuscleCell+PCSK9 OE secretes⏫calcific #ExtracellularVesicle

#Atherosclerosis 2022
www.atherosclerosis-journal.com/article/S002...

@JenniferLees85 presents her work on #vascularcalcification & #vitaminK @Jamietraynor01 @drpaddymark @Kidney_Research @UofGICAMS #eraedta18