New article:
This study reveals that EndMT contributes to atherosclerosis by generating osteogenic- and chondrogenic-like cells, linking endothelial dysfunction to vascular calcification in disease
#EndMT #Atherosclerosis #VascularBiology #CardiovascularScience
doi.org/10.1016/j.vp...

New study reveals that microplastics can worsen pulmonary hypertension by promoting EndMT, inflammation, and histone lactylation in endothelial cells. Key genes like TNFRSF11B and SGK1 emerge as potential therapeutic targets to counter PH progression.
#PulmonaryHypertension #EndMT #Microplastics

#HutchinsonGilfordProgeriaSyndrome

#SmoothMuscleCell Progerin transgene drives #EndMT #Atherosclerosis🐭

⏫Aortic Endothelial Cell permeability
⏫Lesional EC infiltr▶️ColIV- & TGFβ1-rich plaque😮

Paracrine of progeroid aortic SMC?😆

#CircAHA 2024
www.ahajournals.org/doi/10.1161/...

Very proud to share our lab’s recently published work in AJP-Heart

Early in my NIH K08 on #EndMT, we found an interesting signal in COL8A1 in athero, w/ biphasic response to TNF

🙏🏻 to ⭐️ postdoc Qian Li for leading us here (& @haoyin.bsky.social for summary!)

journals.physiology.org/doi/full/10....

COL8A1 #EndMT

Human aortic endothelial cells with immobilized, but not soluble, COL8A1 proteins▶️
Elongated morphology
⏫Endothelial/angiogenic transcriptomic features

TNFα-induced #EndMT ⏫by siCOL8A1, ⏬by lentiviral COL8A1

@jeffhsumd.bsky.social #AJPHC 2025
journals.physiology.org/doi/abs/10.1...

I see a little (green) mouse peeping its head out of its nest. #fluoresencefriday #EndMT

Endothelial‐to‐mesenchymal transition in the fetoplacental macrovasculature and microvasculature in pregnancies complicated by gestational diabetes Abstract figure legend Transforming growth factor (TGF)-β2 and interleukin (IL)-1β induced morphological and molecular changes consistent with endothelial-to-mesenchymal transition (EndMT) in HUVECs ...

Recent paper from our team investigating endothelial-to-mesenchymal transition in fetoplacental vasculature in pregnancies complicated by gestational diabetes #pregnancy #placenta #EndMT #reprorocks

physoc.onlinelibrary.wiley.com/doi/full/10....

Vascular Rarefaction precedes #Cachexia Cancer-induced #SkeletalMuscle Wasting

A remote Tumor (⏫Activin A)-Muscle (⏬#EndothelialCell PGC1α) crosstalk

Lenti-Cdh5-PGC1α intramuscular⏬Cachexia

tumor⏫ SkM EC #EndMT OXPHOS ROS

#Myosky
@jalees.bsky.social #NatCancer 2025
www.nature.com/articles/s43...

#EndothelialCell Robo4
👉⏬irradiation-induced #EndMT
👉Modulates #HematopoieticStemProgenitorCell quiescence & lineage potentials

Evidence of bEND3🐭microvascular EC

Robo4-KD EC coculture▶️
HSPC ⏫G0 quiescence ⏫apoptosis
⏫Erythrocyte
⏫Megakaryocyte

#CellDeathDis 2024
www.nature.com/articles/s41...

#SingleCellProteomics 🐭 #Aorta #MarfanSyndrome

6X ea ♀️♂️ WT Fbn1C1049G/+
24 Aortic roots▶️4996 cells, 2746 proteins, 487 proteins/cell

7 #SmoothMuscleCell subtypes!🤩
poorly overlapped with scRNAseq SMC subtype

#EndMT in Marfan #EndothelialCell

#AortaEd
bioRxiv 2025
www.biorxiv.org/content/10.1...

Good Morning sunshine......Will you be my friEnd(MT)? Sorry sometimes my obsession with pareidolia gets the better of me #vascularbiology #endothelialcells #EndMT #facesinthings 😍

Endothelial-to-Mesenchymal Transition in Cardiovascular Pathophysiology
#EndMT

#Atherosclerosis
#VascularCalcification
#PeripheralArteryDisease
#PulmonaryHypertension
#ValvularHeartDisease
#MyocardialInfarction
#CardiacFibrosis

Krishna Singh lab #IJMS_MDPI 2024
www.mdpi.com/1422-0067/25...

Tuning VEGFA signaling by FLT1 for multiple cell type coordination in #Zebrafish #CardiacRegeneration
#SciArt🤩

A role of EGR3 (rich in #EndothelialCell Fibroblast Myofibroblast) in #EndMT

@rmarinjuez.bsky.social @dev-journal.bsky.social 2024
journals.biologists.com/dev/article/...

#EndMT #Aortopathy #MarfanSyndrome

A cascade of TGFβ-miR-632-DNAJB6 (#LimbGirdleMuscularDystrophy 1E)-βCatenin in both #EndothelialCell & #SmoothMuscleCell

miR-632 mimics delivery (lipofectamine) to cultured human aortic fragments

#IJMS_MDPI 2023
www.mdpi.com/1422-0067/24...

#EndMT in 3D (perfusable) Vascularized #SkeletalMuscleOnAChip

Do we have an isogenic #hiPSC-derived vascularized muscle model to study myopathy-vasculopathy (de)coupling in ischemia or dystrophy?😆

Simone Bersini lab Bioeng Transl Med 2024
aiche.onlinelibrary.wiley.com/doi/full/10....

#YAP/TAZ #HydrostaticPressure #EndothelialCell

Effects of Pressure (20 mmHg capillary, 55 mmHg arteriole) on 2D & 3D EC behaviors
⏫#SproutingAngiogenesis
mouse aortic ring
3D EC microchannel
YAP/TAZ-dependent

⏫Partial #EndMT

⏫YAP nucleation

#CommsBio 2024
www.nature.com/articles/s42...

#EndothelialCell #Mitochodria #LimbIschemia #EndMT

Thrilled to have played a small role in this exciting story😀

Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition

#ScienceAdv 2024
www.science.org/doi/10.1126/...

Splendid story #ZebrafishRocks 🐟

EGR3 as a mechanotranducing transcription factor (nr4a2b-spp1)⏫protrustive & collective migration of endocardial cell during #Zebrafish #CardiacValveMorphogenesis (AV valve leaflet; OFT leaflet)

#EndMT as well?

#ScienceAdvances 2024
www.science.org/doi/10.1126/...

scRNAseq #EndothelialCell in Ldlr-/- 🐭#DiabeticAtherosclerosis

Does the diabetogenic #HighFatDiet+cholesterol produce a more severe (or unique) mode of #EndMT than conventional HFD?

Do EndMT subclusters display altered insulin signaling?

#FrontCellDevBio 2021
www.frontiersin.org/articles/10....

Targeting #EndMT (Barrier/Mechanics/Inflammation) in vitro using N-cadherin antibody-conjugated natural melanin nanoparticle

HUVEC
HDMVEC
HCAEC

Ignore the title type; Clean in vitro data; wait for in vivo data

#AcsNano 2024
pubs.acs.org/doi/10.1021/...

#EndMT in🐭#Atherosclerosis is⏬in♀️

corr w⏫lesion burden,⏫#EndothelialCell lipid peroxidation+apoptosis+lesion denudation

I tabulate the Sex-dependent findings

Also RNAseq of🐭aortic EC &👤lung EC ♀️ vs♂️

Olga Cherepanova lab #ATVB 2023
www.ahajournals.org/doi/abs/10.1...

#SUMOylation #EndMT

SUMOylation (PIAS2α-SUMO1)-DeSUMOylation (SENP1) is critical for⏬SMAD2 ubiquitin-proteasomal degradation & TGFβ-induced #EndMT (HAEC, HUVEC)

PIAS2α & SUMO1 predominantly in nuclei!

Hongmei Wu, Yitao Qi labs #JBC 2023
www.sciencedirect.com/science/arti...

Common & vascular beds-specific transcriptomic traits of #EndMT in vitro

#EndMT ⏫by TGFβ1+IL-1β
⏬by Acetate🥸

HUVEC/HAEC/HPMVEC

Common: Chemokines (CCL3L1/CCL5/CX3CL1/CXCL10/CXCL11/CXCL3/CXCL5/ICOSLG/TNF) as EndMT Secretome

Jianhua Xiong lab G3 2023
academic.oup.com/g3journal/ad...

#EndMT #Aortopathy #MarfanSyndrome

A cascade of TGFβ-miR-632-DNAJB6 (#LimbGirdleMuscularDystrophy 1E)-βCat in both #EndothelialCell & #SmoothMuscleCell

miR-632 mimics delivery (lipofectamine) to cultured human aortic fragments

#AortaEd

#IJMSMDPI 2023
www.mdpi.com/1422-0067/24...

#Obesity #EndMT

Cdh5-CreERT2:ROSA26-eYFP🐭
Hi fat+Hi sugar diet
up to 52 wk!

Mesenchymalized endothelial progeny (Acta2+Pecam1-eYFP+)⏫in #WhiteAdiposeTissue +strong angiogenic transcript feature🤓

Nicholas Chavkin, Kenneth Walsh lab Front CVMedicine 2023
www.frontiersin.org/articles/10....

#GutMicrobiota #EndMT

E. Coli #Flagellin->
#EndMT #LiverSinusoidalEndothelialCell
Steatosis Inflammation Fibrosis #NAFLD
TLR5/Myd88/Twist1

But why E-cadherin instead of VE-Cad?
And figure indexing……

Dr. Hui Dong, Lungen Lu labs #CMGH 2023
www.sciencedirect.com/science/arti...