High Hydrostatic Pressure Exacerbates Bladder Fibrosis through Activating Piezo1

High hydrostatic pressure exacerbates #BladderFibrosis by activating the mechanosensitive ion channel #Piezo1, thereby positioning Piezo1 as a potential #TherapeuticTarget for #BladderOutletObstruction.

Read more here: doi.org/10.1007/s115...

Join us today @ 12PM for our SCBRM Works in Progress Seminar where Kasoorelope Oguntuyo from Dr. Woojin Han's lab and Charles Mangan from Dr. @allisonbond.bsky.social lab will be presenting! 🧬🧠💪 Location: Annenberg 25-51
#SCBRM #musclebiology #neuroscience #PIEZO1 #oligodendrocytes

🧠 In the developing #Xenopus brain, retinal ganglion axons follow a precise path to the optic tectum. Reducing #Piezo1, a mechanosensitive ion channel, in axons or surrounding tissues, result in axons stalling, wandering, or misrouting.

Geoanna M. Bautista et al. examine #Piezo1 in intestinal smooth muscle cells and propose a new conceptual framework which aims to provide a more nuanced understanding of #gastrointestinal #mechanobiology 🔬 📜

🔗 Read the #SymposiumReview: physoc.onlinelibrary.wiley.com/doi/10.1113/...

Spatial Transcriptional Heterogeneity in the Infarct Core and Its Surrounding Regions Targeting Piezo1 Signals in Rats With Myocardial Ischemia‐Reperfusion Injury We employed spatially resolved transcriptomics to visualize and molecularly characterize the spatial distribution of gene expression profiles in the rats’ left ventricles following MIR injury. Furthe...

#Article in #MedComm
#Spatial Transcriptional #Heterogeneity in the Infarct Core and Its Surrounding Regions Targeting Piezo1 Signals in Rats With Myocardial Ischemia-Reperfusion Injury doi.org/10.1002/mco2...

#MIR #Piezo1 #SingleCell #RNA #sequencing

MagPiezo: A Magnetogenetic Platform for Remote Activation of Endogenous Piezo1 Channels in Endothelial Cells MagPiezo enables wireless activation of endogenous Piezo1 channels without genetic modification using 19 nm magnetic nanoparticles and low-intensity magnetic fields. It generates torque forces at the...

...una plataforma capaz de generar estímulos mecánicos precisos con resolución inédita.

advanced.onlinelibrary.wiley.com/doi/full/10....

#Mechanobiology #Piezo1 #Biophysics (2/2)

#Sunitinib is an effective cancer treatment, but can cause #hypertension & #HeartFailure.

Drs. Amit Manhas, Yu Liu, & Dr. Nazish Sayed at @stanfordcvi.bsky.social restored #PIEZO1 expression to reverse sunitinib-induced #endothelial dysfunction.

🔖 https://bit.ly/4s9xrBG
🗨️ https://bit.ly/4p3izlj

Maternal LPS exposure disrupts offspring gut motility: TLR4–PIEZO1 interaction enhances 5-HT–mediated dysfunction, revealing a novel inflammation-mechanotransduction link.
#GutHealth #TLR4 #PIEZO1 #InflammationResearch #Neurogastroenterology
www.sciencedirect.com/science/arti...

Out now: paper in @natcomms.nature.com applying #DNAPAINT 3D #MINFLUX to study activcation and modulation of the mechano-sensitive ion channel #PIEZO1.

www.nature.com/articles/s41...

The #mechanosensitive channel #Piezo1 in #BMMSCs suppresses #BoneMarrow #adipogenesis and promotes #BoneFormation by blocking the Ccl2–Lcn2 inflammatory loop, linking #mechanotransduction to #inflammation control and bone strength.

#OpenAccess in #STTT: doi.org/10.1038/s413...

Piezo1, a key protein in bone health, boosts fracture healing by enhancing mitochondrial energy production. A new study shows it works through a pathway involving β-catenin and Lars2, offering new ways to treat fractures！👇
#Piezo1 #bone #Lars2
@brjournal.bsky.social
www.nature.com/articles/s41...

Finally, our work reveals a hidden mechanical state of regeneration where tissue stiffening instruct cells to transit into regenerative states...

Far from perfect but always looking forward to hearing from you #Regeneration #Mechanobiology #Piezo1 #Yap1 #Xenopus #FrogPower #Morphogenesis

... then we found that cells sense this stiffening via #Piezo1, which in turn activates #Yap1. This Piezo1–Yap1 cascade relays tissue mechanics into transcriptional programs that support the transition into regenerative bud states...

#SmoothMuscleCell #Piezo1 #Hypertension

#HydrostaticPressure➡️
⏫GPR146➡️
⏫SMC synthetic phenotype
⏫cAMP-CREB-Piezo1➡️⏫Syst/Diastolic Blood Pressure

Evidence from GPR146 KO
SMC-GPR146 KI & KO
AAV2-SM22a-GPR146 +SMC-Piezo1 KO🐭

#CircRes 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

#SmoothMuscleCell #Piezo1 Small intestinal motility

SMC Piezo1 KO in 4~6 wk-old🐭
👉Muscularis thinning
👉⏬Food consumption
👉⏬SMC density w/o⏫apoptosis
👉⏬small intestinal motility
👉⏬Muscularis Orai1/3 Trpc4 IP3R ⏫Cav1.2 Ano1
👉⏬carbachol-induced tension

#CommsBio 2025
www.nature.com/articles/s42...

Activation of Piezo 1 in the Pathogenesis of Glaucoma Glaucoma is an eye disorder that damages the retinal ganglion cells and the optic nerve, and can cause vision loss or blindness. However, a lack of understanding of the underlying pathogenetic mechanisms has hampered the development of effective therapies.

🔓📄 'Activation of Piezo 1 in the Pathogenesis of Glaucoma' - an article published in 'Neuropharmacology and Therapy' on #ScienceOpen:

🖇️ #GlaucomaResearch #Piezo1 #CalciumSignaling #Cofilin

LPS exposure during pregnancy affects #IntestinalMotility in offspring mice by activating #TLR4 pathways in the colon. This activation, upon interaction with #PIEZO1, causes calcium influx and increases 5-HT secretion from enterochromaffin cells. #medsky
#OpenAccess:

High Hydrostatic Pressure Exacerbates Bladder Fibrosis through Activating Piezo1

This study reveals that #Highhydrostaticpressure may exacerbate #BladderFibrosis through the activation of #Piezo1, which may serve as a potential therapeutic target for mitigating bladder fibrosis associated with #BladderOutletObstruction. #medsky

#MostDownloaded: link.springer.com/article/10.1...

LPS exposure during pregnancy affects #IntestinalMotility in offspring mice by activating #TLR4 pathways in the colon. This activation, upon interaction with #PIEZO1, causes calcium influx and increases 5-HT secretion from enterochromaffin cells. #medsky
#OpenAccess:
buff.ly/Ib6xt0R

Tissue geometry spatiotemporally drives bacterial infections.

Cellular traction forces modulate heterogeneous #Piezo1 localization. Piezo1 accelerates the formation of bacterial marginal invasion patterns

www.cell.com/cell/abstrac... #MicroSky

神経細胞の新たな移動メカニズムに関する研究成果 近畿大学と京都大学の研究チームが、神経細胞の移動方法を多様化させるメカニズムを解明しました。この成果は脳疾患治療に期待されます。

神経細胞の新たな移動メカニズムに関する研究成果 #大阪府 #東大阪市 #神経細胞 #脳発生 #PIEZO1

近畿大学と京都大学の研究チームが、神経細胞の移動方法を多様化させるメカニズムを解明しました。この成果は脳疾患治療に期待されます。

Wickström @sarawickstrom.bsky.social and colleagues show the role of Piezo1 in ensuring skin barrier integrity by balancing tension. #SkinBarrier #Mechanobiology #Piezo1 @mpi-muenster.bsky.social @helsinki.fi
@reviewcommons.org #preprintpeerreview here >> eeb.embo.org/doi/10.1101/2024.11.20.624378

#SmoothMuscleCell Mechanobiology #Aging #Piezo1

Aortic SMC from aged 🐭> 1 yr (vs 12 wk)
⏬force generation
⏬contractile markers
⏫matrix genes
⏫stiffening marker🥸(ACTG2 ACTN2)
⏫Piezo1

siPiezo1 rescued force dynamics of aged SMCs😎

#AgingCell 2023
onlinelibrary.wiley.com/doi/10.1111/...

#Piezo1 #YAP Bioenergetics

Human Umbilical Cord MSC

#CyclicStretch (10% strain, 0.5Hz, 7 d)
#Piezo➡️
YAP nuclear import➡️
⏫MFN1/2 ⏬DRP1➡️
⏫Mitochondrial fusion ⏫mtDNA-encoded genes➡️
⏫OXPHOS (⏫Glycolysis)➡️
⏫SMC contractile proteins

#FASEBJ 2025
faseb.onlinelibrary.wiley.com/doi/10.1096/...

An interesting #Piezo1 - NF-κB feedforward loop in Vibration-induced injury (Inflammation, Apoptosis) of arterial #SmoothMuscleCell

NF-κB transcriptionally activates Piezo1?

Vibration injury
Rat tail 125 Hz accel 49 m/s2
👤umbilical SMC 125 Hz 5.9 m/s2

#CommsBio 2025
www.nature.com/articles/s42...

Polyunsaturated fatty acids suppress PIEZO ion channel mechanotransduction in articular chondrocytes Chondrocytes sense mechanical signals from their environment via ion channels such as TRPV4 and Piezo1. The mechanosensitivity of these channels is altered by the presence of polyunsaturated omega-3 ...

Congrats to Gabi Marushack and @alisavadipour.bsky.social & team for our latest @Guilak_Lab paper showing that polyunsaturated fatty acids alter cell membrane properties & inhibit chondrocyte #mechanotransduction by #Piezo1 and #TRPV4. faseb.onlinelibrary.wiley.com/doi/10.1096/...

Amyloidβ Aβ1-40 activates #Piezo1 channels in brain capillary #EndothelialCell via ROS

Another splendid patch-clamp study from @osamaharraz.bsky.social

Aβ1-40 doesn't have to be in the pipette to activate Piezo1, likely an indirect effect

@biophysj.bsky.social 2024
www.cell.com/biophysj/abs...

"Overall, antagonizing Piezo1 expression considerably restored the old cells' mechanosensing properties to resemble that of the young cells (Figure 5j)." #aging #longevity #Piezo1 @doctorjosh.bsky.social

#SpaceVascularBiology #VascularAging

#VesselOnAChip
#EndothelialCell +PET membrane + #SmoothMuscleCell

#Microgravity (clinostat-simulated)
⬆️ #Senescence EC SMC
rescued by
1️⃣Laminar Shear
2️⃣EC-SMC co-culture
3️⃣ siRNA #Piezo1 in SMCs

Biosens Bioelectron 2024
www.sciencedirect.com/science/arti...

Looking forward to visiting David Geffen School of Medicine at #UCLA tomorrow. If you are in town and interested in learning about our research on #Piezo1 channels in the brain vasculature and how they affect neurovascular coupling and brain blood flow, I hope this will be of interest.