#OsteoArthritis
InterMuscular Adipose Tissue from Vastus lateralis
6♀️case + 5♀️ctrl

Single-nuclei RNAseq IMAT▶️
Adipocyte, ~20% of non-myogenic cells
highest DEGs
⏫Leptin (➡️FAP/Fibroblast)
⏫Periostin, Ephrin A5 (➡️Mural cell)
⏬IL-16 (➡️Mφ)

#AgingCell 2025
onlinelibrary.wiley.com/doi/10.1111/...

Low-Protein, High-Carb Diet Linked to Longer Life New study finds low-protein, high-carb diets with fiber and flexible eating may extend lifespan, reduce disease risk, and protect long-term health.

🥣 New research reveals a low-protein, high-carb diet with added fiber and flexible eating may support longer, healthier living.

#Longevity #NutritionScience #AgingCell #Healthspan #Biohacking

biohackingnews.org/science/low-...

#MicrovascularAging 🐴Tendon

Young 2~5 y/o
Aged 18~22 y/o
Superficial digital flexor tendon

⏬μCT microvascular (φ>=37 μm) density, φ
⏫VWF+ microvessels
⏫PDGFRβ+ pericytes
⏬Des+/Myh11+ mural cells

5 mm cubes for 3D immunostaining

#AgingCell 2025
onlinelibrary.wiley.com/doi/10.1111/...

#Senescence🦴

p21Cip1- versus p16Ink4a-mediated senescence in radiation-induced #Osteoporosis

Ablating p21+ cells partially rescues
#BoneMineralDensity
#MineralAppositionRate
Trabecular architecture
#BoneMarrowAdiposity
in irradiated🐭🦴

#AgingCell 2022
onlinelibrary.wiley.com/doi/10.1111/...

Heart #Lymphangiogenesis #Aging

#Exercise (6-wk voluntary wheel running) in aged🐭
⏫Lymphatic vessel density
⏬epicardial lymphatic φ
⏬peri-lymphatic immune cells
⏬peri-lymphatic fibrosis
⏫peri-lymphatic cardiomyocyte size
⏫cardiac lymph flow

#AgingCell 2025
onlinelibrary.wiley.com/doi/10.1111/...

Identifying Age‐Modulating Compounds Using a Novel Computational Framework for Evaluating Transcriptional Age RNAge is a transcriptional based computational approach to measure changes in cellular age upon experimental manipulation. In silico screening of a database of transcriptional changes from drug pertu....

Our recent paper in #AgingCell with @studerl.bsky.social where we identify age inducing compounds for improved modeling of neurodegeneration.

Big congratulations to Chao Zhang (now PI at Boston University) and Nathalie Saurat (now PI at Monash University) who led this work.
doi.org/10.1111/acel...

#RNADiscoveryCenter #RNAResearch #Genetics #AgingCell #PiwiGenes #ScientificInnovation #RNA #UNC #UNCChapelHIll #research

Increased H3K9me3-marked #Heterochromatin & altered Epigenome/transcriptome in #AlzheimersDisease Brain (Neuron✅Astroglia❌)

H3K9me3⏬/mRNA⏫
Transcription/chromatin dynamics: HBP1 FOXP1 EHMT1 REPIN1
Cytoskeleton: DES SEPT4 KIFC IQGAP1
PTBP1

#AgingCell 2020
onlinelibrary.wiley.com/doi/10.1111/...

#SmoothMuscleCell Mechanobiology #Aging #Piezo1

Aortic SMC from aged 🐭> 1 yr (vs 12 wk)
⏬force generation
⏬contractile markers
⏫matrix genes
⏫stiffening marker🥸(ACTG2 ACTN2)
⏫Piezo1

siPiezo1 rescued force dynamics of aged SMCs😎

#AgingCell 2023
onlinelibrary.wiley.com/doi/10.1111/...

Cellular #Senescence & Compromised #TightJunction in #SubmandibularGland in🐭Natural #Aging (18-mo) or D-galactose (for 8 wk)

Interestingly, SA β-Gal activity was only evident in aged SMG, not parotid or sublingual glands.

#AgingCell 2025
onlinelibrary.wiley.com/doi/full/10....

Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice This study investigates the circadian rhythm and proteomic profile of aged FKBP51 knockout mice. The absence of FKBP51 provided protection against stress-mediated disruptions in circadian rhythms in ...

Check out our recent paper, out in this month's issue of #AgingCell. Through deep proteomic analysis, we uncovered previously unknown connections between FKBP51 and Mycbp2, Spryd3, and Fbxo45, which may contribute to the #resilience in FKBP51 knockout mice. onlinelibrary.wiley.com/doi/10.1111/...

Lack of adverse vascular phenotypes in mice with postnatal induction of #EndothelialCell #Progerin
Lmna LCS/LCS::Cdh5-CreERT2
Tamoxifen 1.5-mo
Monitored till 22-mo

Normal lifespan
No #Progeria
No Cardiac Fibrosis
No aggravated Atherosclerosis

#AgingCell 2024
onlinelibrary.wiley.com/doi/10.1111/...

#EndothelialCell (Cdh5+) mTOR KO mice->

⏬Macrovascular & Microvascular #VascularAging (ROS, SASP, NO-Vasodilation)
⏫Glucose/Insulin/Pyruvate/Intralipid tolerance

Lifespan? Muscle? Hematopoiesis? Organ-specific Angiocrine?😁

#AgingCell 2023
onlinelibrary.wiley.com/doi/full/10....

Love to see the evidence for pro-Aging/pro-Senescence ECM😁

Pls take a look at my 2nd paper in Pickering lab
"Non-digestible" Collagen matrix⏫#ArterialAging
Collagenase‐resistant collagen promotes🐭aging & vascular cell senescence

#AgingCell 2014
onlinelibrary.wiley.com/doi/full/10....

#Piezo1 drives Mechano-Aging of #SmoothMuscleCells
🐭Aortic SMC 3 mo vs 1 yr

Single-cell spatiotemporal force measurement by
#UltrasoundTweezer PDMS #MicropillarArray

#InstantaneousFrequencySpectrumAnalysis #HilbertHuangTransform

#AgingCell 2023
onlinelibrary.wiley.com/doi/10.1111/...

#Sirt6 #Ovary

#Sirt6 KO🐭->
Ovarian hypoplasia (How?)

1⃣Aberrant ovarian folliculogenesis, trapping at primary follicle status, ⏬follicle collagen thickness

2⃣⏬Estrogen synthesis by in vitro #GranulosaCell, but⏫plasma E/E2 in vivo

#AgingCell 2023
onlinelibrary.wiley.com/doi/10.1111/...