☕ @kutaylab.bsky.social & co identify #lamin B receptor (LBR) and lamina-associated polypeptide 2 (LAP2) as major factors that tether #heterochromatin to the envelope. Deletion of these proteins causes changes in 3D #genome organization, gene expression and cell fate determination.
bit.ly/4aPkNBc
Epigenetics Update - LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis go.nature.com/4kVABWm
Ulrike Kutay (ETH Zurich) in Nat Cell Biol
#Epigenetics #Heterochromatin #Chromtin
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Perfect for cancer, immunology, and aging; epigenometech.com
Top: FK506-resistant strains isolated from the Mucor circinelloides complex. Phenotypic analysis of FK506-resistant isolates in Mucor janssenii CBS185.68 (PS1), Mucor bainieri CBS293.63 (PS3), and Mucor atramentarius CBS202.28 (PS6). WT, wild-type; M, mendelian mutant; E, epimutant. Bottom: Schematic overview of epigenetic silencing contributing to antifungal resistance in Mucor species. fkbA expression enables FKBP12 production and FK506 sensitivity (yeast-like morphology). Epigenetic silencing of fkbA via RNAi and/or heterochromatin leads to FK506 resistance and hyphal growth. Generated with BioRender.com.
#Antifungal resistance is a global threat, but epigenetic mechanisms drive rapid, reversible adaptation in fungi. This study shows that #RNAi or #heterochromatin driven #epimutations transiently silence the gene fkbA to confer FK506 #tacrolimus resistance in #Mucor @plosbiology.org 🧪 plos.io/4qdttWc
Top: FK506-resistant strains isolated from the Mucor circinelloides complex. Phenotypic analysis of FK506-resistant isolates in Mucor janssenii CBS185.68 (PS1), Mucor bainieri CBS293.63 (PS3), and Mucor atramentarius CBS202.28 (PS6). WT, wild-type; M, mendelian mutant; E, epimutant. Bottom: Schematic overview of epigenetic silencing contributing to antifungal resistance in Mucor species. fkbA expression enables FKBP12 production and FK506 sensitivity (yeast-like morphology). Epigenetic silencing of fkbA via RNAi and/or heterochromatin leads to FK506 resistance and hyphal growth. Generated with BioRender.com.
#Antifungal resistance is a global threat, but epigenetic mechanisms drive rapid, reversible adaptation in fungi. This study shows that #RNAi or #heterochromatin driven #epimutations transiently silence the gene fkbA to confer FK506 #tacrolimus resistance in #Mucor @plosbiology.org 🧪 plos.io/4qdttWc
Top: FK506-resistant strains isolated from the Mucor circinelloides complex. Phenotypic analysis of FK506-resistant isolates in Mucor janssenii CBS185.68 (PS1), Mucor bainieri CBS293.63 (PS3), and Mucor atramentarius CBS202.28 (PS6). WT, wild-type; M, mendelian mutant; E, epimutant. Bottom: Schematic overview of epigenetic silencing contributing to antifungal resistance in Mucor species. fkbA expression enables FKBP12 production and FK506 sensitivity (yeast-like morphology). Epigenetic silencing of fkbA via RNAi and/or heterochromatin leads to FK506 resistance and hyphal growth. Generated with BioRender.com.
#Antifungal resistance is a global threat, but epigenetic mechanisms drive rapid, reversible adaptation in fungi. This study shows that #RNAi or #heterochromatin driven #epimutations transiently silence the gene fkbA to confer FK506 #tacrolimus resistance in #Mucor @plosbiology.org 🧪 plos.io/4qdttWc
HP1 isoforms direct repair pathway choice in response to #heterochromatin double-strand breaks. New study from Darshika Bohra and Aprotim Mazumder @tifrhyderabad.bsky.social: rupress.org/jcb/article/...
#Chromatin #epigenetics #DNAbiology #DNArepair
Epigenetics Update - Stress controls heterochromatin inheritance via histone H3 ubiquitylation go.nature.com/4pwTxvp
Shiv I. S. Grewal reporting in Nature
#Epigenetics #Heterochromatin #Ubiquitylation
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Gain deeper insights into gene regulation; epigenometech.com
In @jcb.org, Bohra and Mazumder @tifrhyderabad.bsky.social show that HP1 protein dynamics at #heterochromatin damage sites differentially regulate recruitment of DSB repair factors. Disruption of phosphorylation S11–S14 residues of HP1α impairs homologous recombination. rupress.org/jcb/article/...
Bohra and Mazumder @tifrhyderabad.bsky.social show that HP1 protein dynamics at #heterochromatin damage sites differentially regulate recruitment of DSB repair factors. Disruption of phosphorylation S11–S14 residues of HP1α impairs homologous recombination. rupress.org/jcb/article/...
#Chromatin
Very interesting pre-print from Chris Baker's lab at JAX on the mechanistic link between trans-acting genetic variants and heterochromatin formation in mice. #heterochromatin #epigenetics #variants www.biorxiv.org/content/10.6...
Epigenetics Update - Heterochromatin boundaries maintain centromere position, size and number go.nature.com/3MnWTTh
Lars E. T. Jansen (University of Oxford) reporting in NSMB
#Epigenetics #Heterochromatin #Chromatin
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Discover the breakthrough at epigenometech.com
We set out to understand how subtelomeric #heterochromatin is organized in #Spombe. Subtelomeres are repetitive, variable, and poorly mapped, yet play essential roles in protecting chromosome ends and regulating genome function. (1/n)
Epigenetics Update - Requirements for establishment and epigenetic stability of mammalian heterochromatin bit.ly/425mFRw
Danesh Moazed (Harvard) reporting in Mol Cell
#Epigenetics #Heterochromatin #Chromatin
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Empower your research with high-res epigenetic insights at epigenometech.com
Recent publications from DRN members, Aug–Sept 2025
#Heterochromatin, #Drosophila #olfaction, #pluripotency extinction, #anatomy #education, #zebrafish olfactory #ionocytes, #hearing loss, #machine #learning and more!
See thread for details ⬇️
Sunil Kumar Pradhan @sunil-k-pradhan.bsky.social, M Cristina Cardoso and co-workers from the Technical University of Darmstadt show that dynamic association of H3K36me3 with pericentromeric #heterochromatin regulates its #replication time.
www.embopress.org/doi/full/10....
Epigenetics Update - Dynamic association of H3K36me3 with pericentromeric heterochromatin regulates its replication time bit.ly/3IhhrLw
From M Cristina Cardoso (University of Darmstadt)
#Epigenetics #H3K36me3 #Heterochromatin
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Gain deeper insights into gene regulation; epigenometech.com
Congratulations Manuel and Baizabal lab for this 2025 seed grant from the Brain Research Foundation! 👏🏽🥳
www.thebrf.org/seed-grants/
@iubiology.bsky.social #iu #heterochromatin
📣Rush to read our latest paper!! journals.plos.org/plospathogen.... You'll learn why #heterochromatin is formed and maintained in specific regions of the Plasmodium falciparum genome and not others 🧵⬇️
#LiquidLiquidPhaseSeparation #Heterochromatin
CK2-dependent N-term Ser11-Ser14 phosphorylation & basic segment b4 (K68-K72) of HP1α is critical for its LLPS & heterochromatin assembly
Yoshifumi Nishimura lab #NucAcidRes 2025
academic.oup.com/nar/article/...
Nice review about alternative roles of nucleolus in genome organization in stem cells
Emerging roles for the nucleolus in development and stem cells | Development
#Review #Heterochromatin #GenomeOrganization #StemCells
journals.biologists.com/dev/article/...
#Heterochromatin #Mechanobiolgy
Pulsatile #LaminarShearStress➡️ sustained ⏫SUV39H1 (histone methyltransferase) & H3K9me3 at inflammatory genes (SELE VCAM1) in #EndothelialCell
SUV39H1 physically interacts with Emerin➡️⏫H3K9me3 at nuclear periphery
@pnas.org 2025
www.pnas.org/doi/10.1073/...
H3K27me3 domains in HEK293 cells change with accelerated cell cycle timing. Top left: Flow cytometry analysis of DNA content using propidium iodide fluorescence for HEK293 cells that were treated with DMSO for 48 h. Bottom left: As above, but HEK293 cells treated with Chiron-124 for 48 h. Top middle: Immunoblot for H3K27me3 and H3 on acid-extracted histones after 48-h treatment with Chiron-124. Bottom middle: Quantification of the modification levels normalized to DMSO-treatment. p-value calculated using two-tailed, homoscedastic student t test. Right: Heatmap of H3K27me3 enrichment at domains defined in DMSO treated cells for DMSO treatment (left) and Chiron-124 treatment (middle), and the log2 ratio of H3K27me3 within domains for Chiron-124 over DMSO (right).
Why do #StemCells have lower levels of facultative #heterochromatin, defined by H3K27me3, compared to differentiated cells? By artificially lengthening G1 phase, @4everbiochemist.bsky.social &co show that G1 length is an essential determinant of H3K27me3 landscape @plosbiology.org 🧪 plos.io/4lKfztO
H3K27me3 domains in HEK293 cells change with accelerated cell cycle timing. Top left: Flow cytometry analysis of DNA content using propidium iodide fluorescence for HEK293 cells that were treated with DMSO for 48 h. Bottom left: As above, but HEK293 cells treated with Chiron-124 for 48 h. Top middle: Immunoblot for H3K27me3 and H3 on acid-extracted histones after 48-h treatment with Chiron-124. Bottom middle: Quantification of the modification levels normalized to DMSO-treatment. p-value calculated using two-tailed, homoscedastic student t test. Right: Heatmap of H3K27me3 enrichment at domains defined in DMSO treated cells for DMSO treatment (left) and Chiron-124 treatment (middle), and the log2 ratio of H3K27me3 within domains for Chiron-124 over DMSO (right).
Why do #StemCells have lower levels of facultative #heterochromatin, defined by H3K27me3, compared to differentiated cells? By artificially lengthening G1 phase, @4everbiochemist.bsky.social &co show that G1 length is an essential determinant of H3K27me3 landscape @plosbiology.org 🧪 plos.io/4lKfztO
H3K27me3 domains in HEK293 cells change with accelerated cell cycle timing. Top left: Flow cytometry analysis of DNA content using propidium iodide fluorescence for HEK293 cells that were treated with DMSO for 48 h. Bottom left: As above, but HEK293 cells treated with Chiron-124 for 48 h. Top middle: Immunoblot for H3K27me3 and H3 on acid-extracted histones after 48-h treatment with Chiron-124. Bottom middle: Quantification of the modification levels normalized to DMSO-treatment. p-value calculated using two-tailed, homoscedastic student t test. Right: Heatmap of H3K27me3 enrichment at domains defined in DMSO treated cells for DMSO treatment (left) and Chiron-124 treatment (middle), and the log2 ratio of H3K27me3 within domains for Chiron-124 over DMSO (right).
Why do #StemCells have lower levels of facultative #heterochromatin, defined by H3K27me3, compared to differentiated cells? By artificially lengthening G1 phase, @4everbiochemist.bsky.social &co show that G1 length is an essential determinant of H3K27me3 landscape @plosbiology.org 🧪 plos.io/4lKfztO
#Heterochromatin #Mechanobiology
HP1α, via its dimerization, serves as a chromatin crosslinker
Interphase: P1α & Histone methylation independently contribute to nuclear mechanics
Mitosis: HP1α depletion⏬mitotic chromosome stiffness
@elife.bsky.social 2021
elifesciences.org/articles/63972
#Heterochromatin #Mechanobiology
Distinguish the contributions of HP1α & H3K9me in chromatin condensate formation & biophysical properties
HP1α drives the segregation of unmodified & H3K9me3-marked nucleosomes (fascinating chromatin array assays😍)
bioRxiv 2025
www.biorxiv.org/content/10.1...
Increased H3K9me3-marked #Heterochromatin & altered Epigenome/transcriptome in #AlzheimersDisease Brain (Neuron✅Astroglia❌)
H3K9me3⏬/mRNA⏫
Transcription/chromatin dynamics: HBP1 FOXP1 EHMT1 REPIN1
Cytoskeleton: DES SEPT4 KIFC IQGAP1
PTBP1
#AgingCell 2020
onlinelibrary.wiley.com/doi/10.1111/...
The molecular basis of heterochromatin assembly and epigenetic inheritance | Molecular Cell
#Heterochromatin #EpigeneticInheritance #Review
www.sciencedirect.com/science/arti...
Our work therefore identifies a #KZFP that regulates #enhancer function through targeting domesticated #transposon sequences and operates in a #heterochromatin independent manner.
It also suggests a molecular mechanisms that may underlie the genetic association between ZKSCAN3 and #schizophrenia.
#KZFPs typically function through establishment of #heterochromatin, via interactions with KAP1/Trim28.
However:
- ZKSCAN3 doesn't bind KAP1
- ZKSCAN3 modulation does not affect H3K9me3
- The KRAB-deletion mutant rescues ZKSCAN3 function as well as the wild-type.
