🌟New Product! Recombinant human nodal (Qk029), full length highly bioactive animal origin-free protein.

View and buy online > zurl.co/aYixs

#stemcells #recombinantproteins #pluripotency

Naïve-like H9 hESC colonies exhibit distinct spatial patterns of pan Kla, LDHA, proteins involved in lactate transport, and general pluripotency markers. (A) Schematic showing the process of z-stack selection [left; created in BioRender by Betts Laboratory (2026); https://BioRender.com/k478v5i] and colony section assignment (right) for quantitative analysis of mean intensity. This figure was sublicensed under CC-BY 4.0 terms. (B) Representative immunofluorescence images of untreated naïve-like H9 hESC colonies stained for DAPI (nuclear marker), Pan Kla (global lactylation), LDHA, CX43, MCT1, SOX2, and OCT4. Scale bars: 50 μm.

Alexandra Kozlov, Robert Cumming & co from @westernu.ca examine the levels and spatial distribution of a newly identified protein modifier, lysine lactylation, and its relationship to cell fate in human embryonic stem cells. doi.org/10.1242/bio....

#PluripotentStemCells #Pluripotency #Lactylation

In a new study published in Fundamental Research, a team led by Yi-Liang Miao has pinpointed PIAS4, an E3 ubiquitin ligase, as a pivotal regulator of pluripotency exit in porcine embryonic stem cells. Learn more: https://ow.ly/3Ihp50XQz6H
#Pluripotency exit #Stem #Cells #CRISPR screening

Identification of direct OCT4 targets through integration of OCT4 ChIP-seq and nascent RNA-seq data

Cell Regen Research | 🎯Direct Oct4 targets in ESCs revealed! Auxin-inducible degron (AID) system + nascent RNA-seq uncovers immediate transcriptional changes in pluripotency genes & non-coding RNAs. #StemCells #Pluripotency
🔗 Full paper at: doi.org/10.1186/s136...

Online now! First derivation of nuclear transfer embryonic stem cells (ntESCs) from cloned blastocysts generated by somatic cell nuclear transfer in the #marmoset; ntESCs show normal karyotypes & #pluripotency. https://ow.ly/Xkus50XrM7Z

ISSCR | The Gairdner Foundation | SickKids Foundation

Loss-of-function analysis of the #Oct4 distal (DE) and proximal enhancers (PE) shows that Oct4 PE and DE are required for primed and naive pluripotency, respectively. Powerful genetic tools to dissect Oct4’s role in #pluripotency & early development. https://ow.ly/JShz50Xo5nz

ISSCR

Online now! An evolutionary conserved role for EZH2-polycomb repressive complex 2 (PRC2) in safeguarding #pluripotency from primitive #endoderm cell fate. #hypoblast #transdifferentiation https://ow.ly/Q2eZ50XkBks

ISSCR | Cell Press | SickKids Foundation | The Gairdner Foundation

We are excited to have Prof. Dasaradhi Palakodeti from inSTEM, Bengaluru delivering our monthly Seminar. He will discuss about the mechanisms behind pluripotency in Stem cells.
#stemcells #pluripotency #seminars #invitedtalks

Happy Oct 4 y’all
#oct4 #pluripotency

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

💫NEW: @sarawickstrom.bsky.social @katemiro.bsky.social & co show that mechano-osmotic changes in the #nucleus induce general #transcriptional repression and prime #chromatin for cell fate transitions by relieving repression of specific differentiation genes. #pluripotency
bit.ly/3VMcyNZ

Derivation of embryonic stem cells across 8 avian species including chicken.

buff.ly/ZA7QdIy
#ESCells #PSCells #Pluripotency

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

Recent publications from DRN members, Aug–Sept 2025

#Heterochromatin, #Drosophila #olfaction, #pluripotency extinction, #anatomy #education, #zebrafish olfactory #ionocytes, #hearing loss, #machine #learning and more!

See thread for details ⬇️

New study identifies N-myristoyltransferase (NMT) as a novel regulator of pluripotency, with distinct roles in mice and humans. #stemcells #epiblast #pluripotency ow.ly/IJYb50WNONG

@isscr.org @cellpress.bsky.social @gairdnerawards.bsky.social @sickkidsvs.bsky.social

Metabolic Crossroads: AMDHD2 Couples the Hexosamine Biosynthetic Pathway to Acetyl-CoA Homeostasis in Pluripotent Stem Cells Pluripotent stem cells (SCs) rely on metabolic rewiring to regulate self-renewal and differentiation. The hexosamine biosynthetic pathway (HBP) integrates multiple metabolic inputs to produce uridine ...

🎉 We’re excited to share our very first preprint!! 🎉
"Metabolic Crossroads: AMDHD2 Couples the Hexosamine Biosynthetic Pathway to Acetyl-CoA Homeostasis in Pluripotent Stem Cells"
👉 doi.org/10.1101/2025...

#FirstPreprint #StemCells #Metabolism #Epigenetics #AcetylCoA #HBP #Pluripotency #NewScience

12% of male human embryonic stem cell lines have lost at least in part their Y chromosome, which could have impact on the differentiation capacity of the cells.
By the team of Nissim Benvenisty
buff.ly/JN4Afpf
#Pluripotency
#CheckYourCells!

BZR1 promotes #pluripotency acquisition and callus development through direct regulation of ARF7 and ARF19.

-> www.embopress.org/doi/full/10....

UPRmt controls the mesenchymal-to-epithelial transition - Nature Metabolism The mitochondrial unfolded protein response (UPRmt) is a transcriptional program that is activated in cells experiencing mitochondrial stress and that helps to return to proteostasis and improve stres...

NEWS&VIEWS | M Bartez, D Chen - ‪@ucberkeleyofficial.bsky.social‬:

The mitochondrial unfolded protein response controls the mesenchymal-to-epithelial transition during the acquisition of #pluripotency 🧫

www.nature.com/articles/s42... ?utm_source=bluesky&utm_medium=social&utm_campaign=natmetab

The mitochondrial unfolded protein response inhibits pluripotency acquisition and mesenchymal-to-epithelial transition in somatic cell reprogramming - Nature Metabolism The mitochondrial unfolded protein response is shown to inhibit pluripotency during somatic cell reprogramming and to inhibit the mesenchymal-to-epithelial transition through c-Jun-mediated histone ac...

RESEARCH | Z Ying, X Liu et al. (Guangzhou Medical University):

The mitochondrial unfolded protein response is shown to inhibit #pluripotency during somatic cell #reprogramming 🧫✨

www.nature.com/articles/s42...

Non-canonical enhancers control gene expression and cell fate in human pluripotent stem cells Enhancers are key gene regulatory elements that ensure the precise spatiotemporal execution of developmental gene expression programmes. However recent findings indicate that approaches to identify en...

Intriguing new pre-print from the @stefanschoenfelder.bsky.social lab on unusual human enhancers. www.biorxiv.org/content/10.1... #pluripotency #epigenetics #transcription

New research reveals #OCT4 -regulated perinuclear mitochondrial clustering is required for mesenchymal-to-epithelial transition process of #pluripotency induction ow.ly/XTUJ50VCUbV

@cellpress.bsky.social @gairdnerawards.bsky.social

Join our lab to study transcriptional regulation in mouse germ cells and embryonic stem cells at @haskoliislands.bsky.social in beautiful Reykjavík!
Apply here: tinyurl.com/34y6jcrd
#epigenetics #TranscriptionFactors #transcriptomics #stemCells #pluripotency

Online now - the Review "Decoding human #ChemicalReprogramming: mechanisms and principles" from Hongkui Deng and colleagues.

#SmallMolecules #Plasticity #Pluripotency #Regeneration

authors.elsevier.com/a/1ks7s3S6Gf...

Qkine recombinant human NRG-1 (neuregulin 1) is a highly pure, bioactive animal origin-free monomer view and buy online > zurl.co/RoqSD

3 for 2 mix and match proteins and kits > zurl.co/MXBcp

#stemcells #pluripotency #recombinantproteins

Tracking and mitigating imprint erasure during induction of naive human pluripotency at single-cell resolution Fischer et al. describe a biallelic, dual-colored fluorescent reporter at an imprinted locus in hPSCs, which monitors imprint stability at single-cell resolution. They report that overexpression of ZF...

An elegant study from the Theunissen lab into approaches to counteract the erasure of vital epigenetic marks in cultured human pluripotent stem cells. www.cell.com/stem-cell-re.... #stemcells #pluripotency #epigenetics #imprinting

Tech Tip: Counting hPSC Aggregates for Maintenance Culture and Differentiation

Our Tech Tip outlines how to count PSC aggregates to determine and adjust the plating densities for maintenance and differentiation. You’ll want to bookmark this thread! 👇 #pluripotency

📺 For a more in-depth breakdown, check out this video: bit.ly/3XU4RXc

Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells Pluripotent stem cells are susceptible to acquiring detrimental epigenetic abnormalities, posing a hurdle to their safe use in biomedical applications. Using pluripotent cells with defined genetic bac...

Our work on the regulation is out www.cell.com/stem-cell-re...! Congrats to our collaborators in the Vierbuchen and Mezey labs. It turns out that genetic variation has to be very much considered if you want to harness stem cells safely in biomedicine. #pluripotency #epigenetics #stemcells

Epigenetics Update - Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee bit.ly/43e9Wgs

#Epigenetics #StemCells #Pluripotency
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Empower your research with high-res epigenetic insights at epigenome.us

👋Our February issue's out!
Cover: #glioblastoma

👉🏼Review on #mitochondria #ProteinImport
👉🏻Viewpoint on #LipidMetabolism
🔬 #HumanEmbryos #NuclearSpeckles #PhaseSeparation #autophagy #mitosis #pluripotency #hematopoiesis #LipidDroplets & more!
www.nature.com/ncb/volumes/...

👋🏿Happy new year and our first 2025 issue's out!
Cover: #chromosomal instability in #cancer

👉Review on #TranscriptionFactors and #cell quiescence
🔬#heterochromatin #embryo #DNArepair #pluripotency #proteostasis #obesity #CellCycle #CellDeath & more!

www.nature.com/ncb/volumes/...