Early Ferroptosis Detection Targeting Lipid Hydrogen Abstraction Ferroptosis is an Fe2+-dependent and lipid peroxidation-mediated regulated cell death. Its early detection is critical for diagnosis and timely intervention. Lipid hydrogen abstraction is the initiati...

Youjun Yang and coworkers
did a great chemical probe design paper: Fluorogenicity cleverly triggered by H-atom abstraction. Congrats to all!

#chembio #fluorescencefriday #realtimechem

pubs.acs.org/doi/10.1021/...

Incredible imaging from the @mind-western.bsky.social team for #fluorescencefriday 👇🧡💚!

For #FluorescenceFriday, some of my favorite sections from a new 10X Xenium run at different locations in the anterior/posterior axis of the mouse brain (credit to Olivia Drake, an MD/PhD student in the lab). Each dot marks the location of a specific type of deep layer excitatory neuron.

Happy #FluorescenceFriday!

✨ This Xenopus 🐸 tadpole came to #FluorescenceFriday serving eye-conic energy 👀
Stage 39 eye stained with a whole rainbow: 💙 DAPI | ❤️ Acetylated tubulin | 💚 RPE | 🩵 Rhodopsin | 💜 Pax6. 📸 Image from Jian Sun.

For #FluorescenceFriday, a recent publication highlight from first author Duby Okonkwo from the Schoenecker lab. Great bone imaging with CISR widefield, 3D rendering, and analysis help from Oleg Kovtun. Thanks for using and acknowledging the CISR! pubmed.ncbi.nlm.nih.gov/41651294/

The winner of OneNeuro's March #FotoFriday #FluorescenceFriday contest is "Unleash our inner programmers, without AI" submitted by @takualiu.bsky.social in the Marina Bedny Lab @jhuartssciences.bsky.social

View the complete gallery of OneNeuro BRAINART images here: www.oneneurojhu.org/art/

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is tight junction protein ZO-1.

Our stem cell lines & plasmids are available at just the cost of shipping to accelerate discovery: https://cell-catalog.allencell.org/

📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social

#fluorescencefriday we are back to amyloid (orange) and blood vessels (green) on a triple KI mouse line (Appnl-f, hMAPT and hApoE3). Much less amyloid in blood vessels than the correspondent ApoE4 mice. By the @mind-western.bsky.social team

The future of human clinical sample research is 3D.

This #FluorescenceFriday data (🔈 sound on!) from Clarence Yapp + Seo Woo Choi shows multiplexed labeling in human tonsil and colon samples imaged on #DALISPIM at 9X.

Register for our 4/30 webinar to learn more: bit.ly/4v9aXC1

Looks like a cosmic explosion… but it’s actually a #zebrafishretina 🔬

Pancone line expressing syGCaMP6f
Confocal, whole-mount prep

Sometimes biology doesn’t need enhancement to look spectacular.

#FluorescenceFriday #Neuroscience #Retina

🟢The crystal structure of the green fluorescent protein, GFP! 🟢
The chromophore is yellow. The protein rigidizes the chromophore, releasing energy as fluorescence.
🎥I made the movie with UCSF Chimera & 1EMA.pdb.
#FluorescenceFriday #FluorescentProtein #GFP #SciComm

Close up of a larval zebrafish gut. In this image, several phagocytes have wrapped themselves along the intestine, and are sending protrusions into the gut. The gut itself is visible as a simple long tube. Individual lips-rich cells are visible in cyan.

It's a rainy Easter and I'm working on draft 22 (😢) of this manuscript, so here's a mental reset #FluorescenceFriday peek at some wonderfully busy magenta-labeled phagocytes crawling along a lipid-rich (in cyan) larval fish intestine.

Check out some cool science images (with music by NF) from a recent @nature.com study. @snaga13.bsky.social, @jbuenrostro.bsky.social, et al. found that epigenetic memory of colitis promotes #ColorectalCancer growth. www.nature.com/articles/s41... #FluorescenceFriday 🧪

Perineural net-labeled cells (white), tyrosine hydroxylase-positive cells (red) and mitochondria labelling (magenta) in some cells of the periacqueductal gray region of a mouse brain

Getting into #FluorescenceFriday.

Interesting decorated perineural net cells surrounding the periacqueductal gray region, along with tyrosine hydroxylase-positive cells.

Cellular Liquid Gold 🔬

This time-lapse shows an A549 cell over-expressing Sec61B, outlining the Endoplasmic Reticulum. Imaged at 1 frame per 500ms, it reveals just how violently active our cellular infrastructure really is!

What is your favorite organelle to image? 👇

#SciArt #FluorescenceFriday

Going through some old microscopy data, and I have to say - I maintain that #mitochondria are the most beautiful organelles, but the #ER might be a very close second.
#FluorescenceFriday

Live HeLa cells, ER imaged by STED, mitochondria with confocal.

Reactive microglia (🟠) and astrocytes (⚪️) as well as Aβ plaques (🔵) in the hippocampal dentate gyrus of a 5xFAD mouse model of Alzheimer's disease.

#FluorescenceFriday

Mouse brain stem section with GLP-1-producing neurons marked in green and low-activity neurons in magenta.

This is a fun #FluorescenceFriday pic. My favorite NTS neurons that express a GLP-1 precursor in Green and the inverse activity marker phospho pyruvate dehydroxylase in magenta. Awesome work by my master's student Patience Otiya.

#FluorescenceFriday
This microscopic🔬 object is a specialized immune cell (microglia) that resides in your retina👁️ and is responsible for damage control and refinement of neural circuits. GFP labeled

@urneuroscience.bsky.social @urochestersmd.bsky.social @flaumeye.bsky.social @uofrbme.bsky.social

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is LAMP-1 - illuminating lysosomes. 🧫 ✨

Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.

📦 Hosted by @coriellinstitute.bsky.social & @addgene.bsky.social

Happy #FluorescenceFriday! Today we are excited to share this video which shows a cleared mouse brain hemisphere following depletion of endogenous microglia and transplantation of human cells (magenta).

The @mind-western.bsky.social #fluorescencefriday entry focus on the hippocampus of an aggressive AD model Appnl-g-f with IBA1 (yellow) and abeta (magenta). thanks to @shahrzadbahrampour.bsky.social @nadiahaghbinphd.bsky.social and @kesummers.bsky.social

We had soooo much fun learning from Steve Farber this week. Please enjoy this zebrafish larval #FluorescenceFriday image with macrophages in red (the extensions!! 😍) and lipid uptake in the gut in green.

microscopy images of a developing mouse spinal cord showing nuclei, actin, and membrane stains in rainbow coloring

Happy #FluorescenceFriday! This is a confocal 🔬 series of a developing 🐭 spinal cord showing nuclei, actin, and cellular membrane stains colored in 🌈 Microscopy by postdoc @christinaadaly.bsky.social 👩‍🔬 🧪

Such a beautiful example demonstrating the importance of developing and using robust biosensors
#FluorescenceFriday

✨ Somite symmetry never fails to mesmerize 🐟
Trunk somites of a 1-day-old zebrafish embryo with nuclear (💙) and membrane (🧡) labelling 📸 Image credit: Matyas Bubna-Litic (Mongera lab, UCL) 🔬 #FluorescenceFriday

#Cilia are beautiful!

That's it, that's the tweet.

Oh, and Happy #FluorescenceFriday 🔬

A dark-blue background with bright red/yellow neurons arranged in a diagonal band through the middle. The right side is faintly striped, typical of the striatum.

Happy #FluorescenceFriday! Since I got to do some rare patching this week, enjoy this sagittal brain slice with ChAT+ interneurons in the Striatum, labelled in pAce-mNeonGreen.

This #FluorescenceFriday post is for those who do 2-photon calcium imaging. Make your videos pop! @filip-tomaska.bsky.social released code and a demo to make the neural activity in your videos clear.
Code: github.com/ftomaska/roi...
Video demo: youtu.be/my1CE9sn2FQ

E.g.: bsky.app/profile/spen...