New Epigenome Technologies Blog post! bit.ly/3P3XI59

“Chamber-specific Epigenetic Analysis Identifies Cardiovascular Disease-associated Regulatory Elements in Cardiomyocytes”

Based on a exciting recent Nature Communications article

#EpigenomeTech #Blog #Epigenetics #CREs #Cardiomyocytes

💓 hiPSC-derived #cardiomyocytes from each index short QT syndrome patient were developed, as well as an isogenic cell line with variant correction and HEK 293T cells transfected with SLC4A3 expression constructs expressing either wild-type SLC4A3 or the variants.

👉 https://bit.ly/46SVMCJ

#HighIntensityIntervalTraining alleviates #heartfailure by boosting skeletal muscle–derived #Metrnl, which activates AMPK-HDAC4 signaling in #cardiomyocytes to enhance GLUT4 expression, aerobic glucose metabolism and mitochondrial function.

#OpenAccess: doi.org/10.1016/j.ge...

💓 Through employing an in vitro model replicating atrial #cardiomyocytes during mitral regurgitation, scientists observed that shear stress increased Ca2+ oscillation and the amplitude of Ca2+ waves through extracellular Ca2+ influx.
https://bit.ly/4tyCxbl

🫀🧫 Two of our favourite things in one place - EBiSC iPSCs and cardiac research - shown here as UKBi005-A iPSC line differentiated to cardiomyocytes stained with cTNT (green) and Connexin-43 (violet). 💚

#EBiSC #iPSC #CardiacResearch #Cardiomyocytes #StemCellResearch #DiseaseModelling

Response of human iPSC-cardiomyocytes to adrenergic drugs assessed by high-throughput pericellular oxygen measurements and computational modeling Rate- and contractility-modulating drugs, such as adrenergic agonists and antagonists, are widely used in the treatment of cardiovascular conditions. …

🫀New research demonstrates that high-throughput, label-free optical oxygen measurements can assess adrenergic effects in iPSC-derived #cardiomyocytes

Paired with all-optical #electrophysiology, this enables scalable, human-based preclinical testing

#CardioSky

www.sciencedirect.com/science/arti...

🫀🦠 How does #COVID19 damage the heart?

New research from Gross et al. using human iPSC-derived #cardiomyocytes uncovers cellular responses to SARS-CoV-2, shedding light on mechanisms of cardiac injury.

www.sciencedirect.com/science/arti...

Myocardial I/R injury stimulates the activation of STING signaling, that binds directly to GPX4, exacerbating ferroptosis in #cardiomyocytes, while targeted inhibition of STING may alleviate subsequent #cardiacdamage.

#STTT #OpenAccess: doi.org/10.1038/s413...

This paper investigates how pre-existing cardiomyocytes may act as central players in regenerative signaling, offering fresh perspectives on how the heart could repair itself after injury. #CardiacRegeneration #Cardiomyocytes #RegenerativeMedicine
www.sciencedirect.com/science/arti...

Minimally invasive retrieval of #cardiomyocytes via ablation catheters enables successful DNA extraction & sequencing, paving the way for #mosaicism detection & novel cardiac genetic research bit.ly/4pPYksf #GIMO #Epigenetics #ArrhythmogenicRightVentricularCardiomyopathy

Inflammation-Related Factors S100A9 and TLR2 in Cardiomyocyte Hypertrophy

A pathogenic S100A9–TLR2 feedback loop amplifies #hypertrophic and #InflammatorySignaling in mechanically stressed #cardiomyocytes, linking immune dysregulation to maladaptive #CardiacRemodeling and highlighting a therapeutic target in #HeartFailure.

Read: doi.org/10.1007/s115...

A new @cp-cellstemcell.bsky.social paper reveals a cardio-immune mechanism whereby Clusterin+ #cardiomyocytes reprogram #macrophages to resolve inflammation.

Read the paper: https://bit.ly/3Kn8GR9
Listen to the discussion: https://bit.ly/48dmghX

Nanoscale architecture and dynamics of CaV1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopy The clustering of L-type calcium channels for functional regulation of intracellular calcium signaling remains poorly understood. Here we applied supe…

Ever wondered how #Calcium channels cluster in #iPSC derived #Cardiomyocytes?

New work just published from Schwenzer et al. should put your mind at rest as they investigated just that!

Check out the latest paper for more information

www.sciencedirect.com/science/arti...

#CardioSky

Wnt/β-catenin signaling regulates cardiac Cx43 in a metabolic substrate-dependent manner Both Nav1.5 and Cx43 are critical for the fast electrical impulse conduction in the myocardium and their reductions create the arrhythmogenic substrat…

Looking for some weekend reading? 📜

New work from from the University of Ottawa Heart Institute, show how metabolic substrates regulate effects of Wnt/β-catenin signaling in #cardiomyocytes, specifically affecting regulation of #Connexin43

sciencedirect.com/science/arti...

This video for the MRC Toxicology Unit @uniofcam.bsky.social was made by one of my students! Go have a watch of this fascinating beautiful video youtu.be/TSLPr74m75Q?... #cardiomyocytes

Close-up of a glowing green cellular structure against a dark blue background under a microscope.

Optimizing cardiomyocyte differentiation from iPSCs can be tricky. Our blog shares tested strategies to improve consistency, and purity, and how high-quality HumanKine® growth factors can support cultures.

Read more: ow.ly/881l50WQi3k

#iPSC #Cardiomyocytes #Proteintech

Human heart-on-a-chip model emulates structural and functional characteristics of diastolic dysfunction and reveals a beneficial role for senolytics Diastolic dysfunction (DD) is a hallmark of heart failure with preserved ejection fraction (HFpEF), a huge burden on healthcare systems worldwide. Usi…

Omar's final PhD paper is finally out! He describes the generation of a 3D in vitro model of diastolic dysfunction w preserved ejection fraction using human #cardiomyocytes.
#hypertrophy #HFpEF #OOAC @uhnresearch.ca @uoftmedicine.bsky.social
Check it out👇
www.sciencedirect.com/science/arti...

This new #Methods article presents a standardized workflow for high-throughput morphological and molecular characterization of isolated #cardiomyocytes
#MachineLearning #Cardiovascular #CellPhysiology #CellMorphology
doi.org/10.14814/phy...

📌 #SARS-CoV-2 #Damage #Mitochondrien #Mitochondria #Cardiomyozyten #Cardiomyocytes

Single molecule imaging reveals extracellular signal-regulated kinases dependent signal responsive spatial regulation of translation in cardiomyocytes Background Translational control of gene expression is crucial in cardiomyocytes, particularly in response to hypertrophic stimuli. The extracellular signal-regulated kinases (ERK) pathway plays a key...

Found in translation: Using single-molecule imaging, we show that ERK signaling controls where protein translation happens during hypertrophic stimulation in #cardiomyocytes —revealing a dynamic, localized response. #Biorxivpreprint #cardiosky
Read: www.biorxiv.org/content/10.1...

Human induced pluripotent stem cell-derived cardiomyocytes and their use in a cardiac organ-on-a-chip to assay electrophysiology, calcium and contractility - Nature Protocols Using human induced pluripotent stem cell-derived cardiomyocytes in an organ-on-a-chip model to assay cardiac function: electrophysiology, calcium and contractility. This protocol aims to provide key ...

#FeaturedProtocol this week: #Cardiomyocytes derived from stem cells and their use in an #organonachip model to assay cardiac function bit.ly/3EoI6Ee

Neda Mohammadi of @imperialcollegeldn.bsky.social et al. found that sympathetic #neurons can modify the intrinsic structural and functional properties of human pluripotent stem cell-derived #cardiomyocytes in their recent study 🔬 📜

Read the #Research here: buff.ly/BlMgoUv

We had a great visit to the lab of Prof. Katja Gehmlich @ Birmingham University to learn about engineered heart tissues using #iPSC-derived #cardiomyocytes. A big thank you to the support by the #DanishCardiovascularAcademy #DCA

Research Tip Sheet: New Single-Cell Proteomics Technology Reveals Heart Cell Differences
www.newswise.com/articles/res...

#cedarssinai #singlecellanalysis #clivesvendsen #eduardomarbán #smidtheartinstitute #advancedprotein #cardiomyocytes #heartmusclecells #rarehybrid #heart #hearthealth

#Reprogramming Cells for #Heart #Repair: New Method Transforms #Fibroblasts into Mature #Cardiomyocytes
Their method relies on combining FGF4 with vitamin C... scitechupdates.com/r...

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

How to fix a broken #heart 💔->❤️

#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.

Happy #Fluorescencefriday #BlueSky & #CardioSky!

@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬

Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes. A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below! https://doi.org/10.1016/j.crmeth.2022.100300 https://doi.org/10.1101/2024.10.17.616637

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

Isolated adult mouse ventricular cardiomyocytes

Was a couple of years since I last isolated some adult mouse #cardiomyocytes, but happy to see that it still works. Based on PMID 27502479. ❤️🔬 #CardioSky

Tomorrow Dr. Carol Ann Remme will shed light on the specificities of membrane targeting of #sodiumchannel in #cardiomyocytes 🫀

#WorldWideSodiumChannelSeminar

link will be available here: sodiumchannelseminars.org/Program-2024...

New science and approaches to decrease cardiometabolic risk! S102a #AHA24 #translationalscience #cardiomyocytes #adipocytes