Human Lung Aging
lungaging.com
60👤
200k cells
25 cell types

Transcriptional Entropy🤠 is superior to gene expression in predicting chronological age in general capillary EC

Somatic mutation burdens 3~7 mut/MB vary across cell types

Naftali Kaminski lab #NatComm 2026
www.nature.com/articles/s41...

#AlternativeSplicing analysis pipeline

MAJIQ v2 - VOILA v2 modulizer

👉Build gene splicegraph including unannotated, complex elements (e.g. de novo intron retention)
👉Quantify AS events & modules
👉Comparative analysis of GTEx v8 all 53 tissues

#NatComm 2023
www.nature.com/articles/s41...

Prime Editing for patient-derived Liver or Intestinal Organoids

Alleles edited
CTNNB1 in-frame 6bpΔ
ABCB11 D482G or R1153H
ATP7B S430fs
SERPINA1 E342K
DGAT1 S210Δ

WGS of 4 prime-edited clonal organoid lines▶️absence of genome-wide off-target effects

#NatComm 2020
www.nature.com/articles/s41...

hiPSC-derived Neural Progenitor Cells support long-term (43 dpi) graft neuronal integration & functional recovery after photothrombotic Stroke🐭

👉57% neurites extend along cc into contralateral hemisphere

Grafts▶️44% GABAergic Neurons

@rrust.bsky.social #NatComm 2025
www.nature.com/articles/s41...

The pyramid of tears and disappointment. #natcomm #embo2025cytoskeleton quotation from @wormlockholmes.bsky.social

GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell

GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain

Myh11+ SMC Gpr153 KO 🐭
Injured artery

Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke

#NatComm 2025
www.nature.com/articles/s41...

Systematic comparisons among 4 subcellular-resolution #SpatialTranscriptomics methods

Stereo-seq v1.3
Visium HD FFPE
CosMx 6k
Xenium 5k

Human Tumors
Ground truth CODEX, +scRNAseq

#NatComm 2025
www.nature.com/articles/s41...

#Pieoz1-TMEM16F ( lipid scramblase)➡️ #Trophoblast Fusion #Polyploidy

Trophoblast Piezo1 KO🐭➡️
👉Embryonic death
👉↔️fetoplacental #Angiogenesis
👉Compromised multi-nucleated Syncytiotrophoblast barrier
⏬MCT4 (maternal-facing BM)
⏬MCT1 (fetal-facing BM)

#NatComm 2025
www.nature.com/articles/s41...

#MoyamoyaDisease

ACTA2 R197C/+🐭
👉Less differentiated #SmoothMuscleCell ⬆️migration (in vitro)
👉SMC are ⬆️glycolytic ⬇️OXPHOS

+Carotid ligation
👉Lethal, occlusive intima
👉Dilated, kinky leptomeningeal collaterogenesis

Rescued by #NicotinamideRiboside

#NatComm 2025
www.nature.com/articles/s41...

Extended coverage of human serum glycosphingolipidome by 4D-RP-LC TIMS-PASEF unravels association with Parkinson’s disease - Nature Communications 4D-TIMS-glycosphingolipidomics expedite clinical serum profiling of 376 GSLs covering ganglio- and neolacto-series, neutrals and sulfatides. Clinical phenotyping of Parkinson’s disease cohort (sub)gro...

#Glycosphingolipidomics alterations iincluding #peripheral #immunity dependant profiles in #Parkinsons disease New Manuscript allert in #NatComm go.nature.com/43AalJZ

#Neurology
#Medicine

SIMO
Integration of multimodal single-cell omics (incld SNARE-seq ISSAAC-seq) with #SpatialTranscriptomics

Modality-specific kNN + fused G-W #OptimalTransport

vs CARD LIGER Scanorama Seurat Tangram

Spatial ATAC-RNAseq
Spatial CUT&TAG-RNAseq

#NatComm 2025
www.nature.com/articles/s41...

Developmental vs Neonatal Hyperoxia-induced #Senescence in🐭Lung

Both p21-dependent
AT2, mesenchymal cell, secondary crest myofibroblasts are senescent

Development
❎DNA damage
❎SASP
Disrupted by p21 KO

Hyperoxic injury
✅DNA damage
✅SASP
⬇️by #Senolytic

#NatComm 2023
www.nature.com/articles/s41...

#Sirt6 #Cartilage Development #Aging #OsteoArthritis

Intra-articular injection of tgg2 (chondrocyte aptamer)-PP (PEGylated PAMAM: drug encapsulator)-MDL800 (Sirt6 activator) #Nanoparticle
Persist up to 40 d in normal🐭
⏬#Chondrocyte #Senescence+🐭OA (DMM)

#NatComm 2022
www.nature.com/articles/s41...