Quantitative mapping of methionine sensitivity to oxidation in the copper-bound PcuC chaperone Copper is typically coordinated by histidine, cysteine, or methionine in proteins, and these residues are particularly sensitive to oxidation. However…

Quantitative mapping of methionine sensitivity to oxidation in the copper-bound PcuC chaperone #RedoxBiol #MassSpec www.sciencedirect.com/science/arti...

Rapid quantitation of NAD+/NADH and NADPH/NADP+ with mass spectrometry by using calibration constants NAD+/NADH and NADPH/NADP+ are important indicators of cellular redox status, but they cannot be reliably calculated from the relative intensities of m…

Rapid quantitation of NAD+/NADH and NADPH/NADP+ with mass spectrometry by using calibration constants #RedoxBiol www.sciencedirect.com/science/arti...

#EndothelialCell #MerTK #Efferocytosis #Atherosclerosis

EC MerTK KO🐭+ AAV8-PCSK9 + #HighFatDiet 2-mo
👉⏫lesion burden
👉⏫NADPH oxidase
👉⏫inflamm cytokines

Aortic Arch lesion #Proteomics➡️
⏫TGFβ HIF1α Wnt3a IL2
⏬LARP1 NAMPT TEAD1

Zufeng Ding lab #RedoxBiol 2025
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Mapping the oxidative stress metabolome in neurology by gas chromatography–mass spectrometry: a systematic review on signature-driven diagnosis and disease monitoring Oxidative stress is a pivotal factor in the pathogenesis of neurological conditions; however, its clinical assessment is constrained by the ephemeral …

Mapping the oxidative stress metabolome in neurology by gas chromatography–mass spectrometry: a systematic review on signature-driven diagnosis and disease monitoring #RedoxBiol www.sciencedirect.com/science/arti...

Ether lipids and sphingolipids drive sex-specific human aging dynamics Aging is a dynamic process characterized by complex molecular changes, including shifts in lipid metabolism. To systematically define lipidome dynamic…

Ether lipids and sphingolipids drive sex-specific human aging dynamics #RedoxBiol #MassSpec www.sciencedirect.com/science/arti...

A high-resolution N-glycoproteome landscape of aging mouse ovary Ovarian aging typically precedes the decline of other organ systems, yet its molecular mechanisms remain poorly understood. Glycosylation as one of th…

A high-resolution N-glycoproteome landscape of aging mouse ovary #RedoxBiol #MassSpec www.sciencedirect.com/science/arti...

Multi-tissue metabolomics reveal mtDNA- and diet-specific metabolite profiles in a mouse model of cardiometabolic disease Excess consumption of sugar- and fat-rich foods has heightened the prevalence of cardiometabolic disease, which remains a driver of cardiovascular dis…

Multi-tissue metabolomics reveal mtDNA- and diet-specific metabolite profiles in a mouse model of cardiometabolic disease #RedoxBiol #MassSpec www.sciencedirect.com/science/arti...

Quantitative analysis of the proteome and protein oxidative modifications in primary human coronary artery endothelial cells and associated extracellular matrix Vascular endothelial cells (ECs) play a key role in physiology by controlling arterial contraction and relaxation, and molecular transport. EC dysfunc…

Quantitative analysis of the proteome and protein oxidative modifications in primary human coronary artery endothelial cells and associated extracellular matrix #RedoxBiol #MassSpec www.sciencedirect.com/science/arti...

Lung #Pericyte #Ferroptosis #Sepsis

A pro-ferroptotic axis
#YAP ACSL4 ⏫PUFA esterification ⏫Phospholipids for #LipidPeroxidation

1⃣Fer-1
2⃣Pericyte Acsl4 KO
3⃣NG2/CD13-targeting LNP to deliver ad-shACSL4
⏬🫁 pericyte loss
⏫Survival
in CLP septic🐭

#RedoxBiol 2024
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Dehydroascorbic acid quantification in human plasma: Simultaneous direct measurement of the ascorbic acid/dehydroascorbic acid couple by UPLC/MS-MS Ascorbic acid (AA, vitamin C) and dehydroascorbic acid (DHA) constitute a biological couple. No technique can accurately, independently, and simultane…

Dehydroascorbic acid quantification in human plasma: Simultaneous direct measurement of the ascorbic acid/dehydroascorbic acid couple by UPLC/MS-MS #RedoxBiol www.sciencedirect.com/science/arti...