A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

Our latest work on shape-programmable tissues is out in @science.org. By positioning topological defects in cellular nematics, we encode frustrated 2D force fields that relax into predictable 3D shapes. Collaboration with Marino Arroyo’s lab, led by @pauguillamat.bsky.social at @ibecbarcelona.eu.

1/ Excited to share our preprint! 🥳

Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine

www.biorxiv.org/content/10.6...

🥳 Excited to share our #diatom work out in @pnas.org. In a great collaboration with @nic-poulsen.bsky.social and @ulrichschwarz.bsky.social we show that diatoms modulate the curvature of their paths by dynamic switching of their cell–substrate contact sites.

doi.org/10.1073/pnas.2506122123

Diffusive spreading across dynamic mitochondrial network architectures | PNAS In eukaryotic cells, mitochondria form networks that range from highly fused interconnected structures to fragmented populations of individual orga...

Excited to share a new collaborative paper with Lena Koslover's team, fueled by @chanzuckerberg.bsky.social Metabolism Across Scales Program 🧪 1/
www.pnas.org/doi/10.1073/...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Congrats to Chunyu Li, Dr Xu-cheng Gan, Yu (Hannah) Irie and Dr Milo Smith for their paper appearing today in Science!

Some molecular machines, like ribosomes, can persist for long periods of time in cells.

Could molecular aging of ribosomes shape how proteins are made?

In our new preprint we track ribosomes as they age in cells and uncover unexpected effects on translation (1/10)

www.biorxiv.org/content/10.6...

Pulsed #lasers integrated with #cryoEM: @fitzpatricklab.bsky.social postdoc Daniel Du aims to boost contrast in #cryoET zuckermaninstitute.columbia.edu/making-world-s-best-microscopes-biology-better
zuckermaninstitute.columbia.edu/science-life-daniel-du
elifesciences.org/reviewed-preprints/109793v1

Evolution navigated billions of challenges to get to us to where we are today. Directed evolution compresses this to a 1D axis.

Imagine if you could sample 200 dimensions at once, with data to boot 📈

First @chorylab.bsky.social PACE preprint on our new system to tackle this: bit.ly/turboprance

The molecular basis of force selectivity by PIEZO2 Nature - PIEZO2 is intrinsically more rigid than PIEZO1, and disparate mechanical stimuli paradoxically evoke opposite conformational and gating responses in each channel.

New paper from the lab led by @ericmulhall.bsky.social addresses how PIEZO1 and PIEZO2 are tuned to different types mechanical forces. From nanometer-scale super-resolution microscopy to in vivo experiments, links single-molecule observations to physiological function.
www.nature.com/articles/s41...

Papers are like buses... You wait for ages, then two come along at once.

Huge congrats to @bornanovak.bsky.social and @jefflotthammer.bsky.social for pushing and driving every aspect of this work, preprinted ~1 year ago to the day (Friday before BPS), now published!

www.nature.com/articles/s41...

Have you ever wanted to do single-molecule biophysics in high-throughput? @matt-dejong.bsky.social (amazing joint grad student with @dunnlab.bsky.social) invented a way & measured >100,000 single molecules across 241 different sequences. (1/3)
www.biorxiv.org/content/10.6...

Thanks so much Samrat!

Single-cell and isoform-specific translational profiling of the mouse brain - Nature Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cell RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hipp...

Chuffed to see our paper with @geneyeo.bsky.social finally published in @nature.com.
www.nature.com/articles/s41...
Very grateful to Fede, Susie, and Dave from my lab, and Sammi, Eric, and Pratibha from Gene's lab. What did we find? ⬇️ ⬇️ ⬇️

New preprint from @kutseikin.bsky.social showing how pharmacologic inhibition of SLC33A1 activates IRE1/XBP1s signaling. w/ @enriquesaez.bsky.social @chrisgparker.bsky.social @landerlab.bsky.social @forlilab.bsky.social & the Birsoy lab (Rockefeller) Check it out👇

www.biorxiv.org/content/10.6...

High-throughput ligand diversification to discover chemical inducers of proximity - Nature Chemical Biology Molecular glue degraders have consistently been discovered retrospectively, despite their increasing importance. Herein, a high-throughput approach is described that modifies existing ligands into mol...

What a thrill to see this out! Our prospective, scalable, and target-centric solution for molecular glue discovery.

More thoughts and details here: www.linkedin.com/posts/activi...

Paper here: www.nature.com/articles/s41...

Pleased to share the final version of this behemoth of a paper, now finally published. I guess I can retire now?
www.nature.com/articles/s41...

More functional data, many thousands of words removed, and a few other updates from last year's preprint.

Thanks so much Samrat!

Bioengineer Clifford Brangwynne wins Keio Medical Science Prize Japan’s Keio University awards the prize annually to honor contributions in medicine and life sciences. Brangwynne is being recognized for groundbreaking work that has opened up a new field of cell bi...

A tremendous honor! Thrilled & humbled to receive 2025 Keio Medical Science Prize for launching LLPS #phaseseparation field (= #softmatter + #cellbio) w collaborators esp @HymanLab. & Congrats to Akiko Iwasaki @virusesimmunity.bsky.social. www.princeton.edu/news/2025/09...
#KeioMedicalSciencePrize

Would be great to be added, thanks!

Selective ion binding and uptake shape the microenvironment of biomolecular condensates Biomolecular condensates modulate various ion-dependent cellular processes and can regulate subcellular ion distributions by selective uptake of ions. However, the molecular grammar governing condensa...

Curious how biomolecular condensates can localize ions? Using NMR, we found that condensates selectively bind and localize both chaotropic anions and kosmotropic cations. This alters the condensate composition, interface potential and RNA duplex stability inside. By @irissmokers.bsky.social

I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/...
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!

Short activation domains control chromatin association of transcription factors Transcription factors regulate gene expression with DNA-binding domains (DBDs) and activation domains. It is generally assumed that DBDs are solely responsible for interacting with DNA and chromatin. ...

Happy new year! Our new preprint is live! We use single molecule imaging in live cells to show that activation domains can control how transcription factors bind to chromatin. SMT 1 TF Dogma 0 www.biorxiv.org/content/10.1...

Programming scheduled self-assembly of circadian materials - Nature Communications Harnessing biomolecular systems to endow synthetic materials with life-like properties is a significant challenge. Here, the authors use the biomolecular KaiABC circadian clock proteins to control aut...

Happy to be on BlueSky!
For my first post, I am excited to share a new collaborative paper combining experiments and simulations, where we used circadian clock proteins to engineer biomaterials with time-programmed self-assembly and disassembly www.nature.com/articles/s41...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

EDUCATION CORNER: Molecule of the Month: Celebrating  25 Years of Storytelling and Announcing New Beginnings After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month series.  Janet Iwasa will be continuing the series for PDB-101.

After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service.
@jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases
Details: cdn.rcsb.org/rcsb-pd...

😊 I feel very lucky that you made that choice Samrat, and it’s fun to look back on the novel science you started then! Congrats on all of your/your lab’s outstanding achievements!

Congrat Alex!

Amazing and creative work by Anthony, who very independently made the novel and exciting discoveries/developments in this paper - big congrats Anthony!

Anthony is a
@scripps.edu Graduate School student (joint with @kerenlasker.bsky.social) - great collaboration - big congrats Keren!