Great paper! It is in my to-do list to read it in details to discuss it with a colleague next week.

New paper out 💥 3D cell-cell contact reconstitution with synthetic vesicles and (primary) immune cells to study how different proteins and carbohydrates influence the immune synapse dynamics.
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Glad im not at FOM!

Would be good to have a chat with us so we can share our analysis too!

When I excite with 405 nm and collect in the 475/50 channel, I basically see nothing, no membrane, no puncta. But when I excite with 488 nm, I clearly see those lysosome-like puncta, and they overlap with LysoTracker Orange. This to me doesn't make sense as Laurdan shouldn't be excited at 488!

Thanks for sharing! I actually know that one, and yeah, I’m not surprised that Laurdan is colocalising with LysoTracker due to internalisation of Laurdan and accumulation in endo/lysosomal compartments.

What I’m still confused about is the excitation/emission behaviour.

Which CAD software has AI tools? I mainly use OnShape and haven’t seen any AI tools to pop up!

0.28

The one you have is UPlanSApo but they have a new version UPlanXApo. It’s very good!

You’ve got to get the new 60X/1.20 W.

Haven’t taken a flight in 14 months ✈️
Won’t take one until April either, which’ll make it 17.
Just a reminder that travel choices do add up for CO₂. One person skipping a flight doesn’t ground a plane, but lower demand over time does mean fewer routes and frequencies.

Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!

Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.

www.biorxiv.org/content/10.6...

Happy holidays ✨🎄

✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.

This is one of the examples of designing a objective lens+tube lens and I was always wondering why they don't include the scan lens in the combo design.

www.oejournal.org/oea/article/...

I’d say there is no obvious reason for adding telecentricity! It would make the lens bulkier (i guess) but wouldn’t change the image quality that much!

Great stuffs!

Tracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff:

Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...

Cool stuff! Congrats!

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos.
www.nature.com/articles/s42...

Interesting! So one can say the GSG's role is purely translational?

Hahaha!

Yup! Over 20 mm range, and at ambient and cryogenic conditions.

The use of hexapod actuators is insane!

www.spiedigitallibrary.org/conference-p...

The telescope design is superb imo!

Reviewer #3: Aberrations are all over the place!

Protein codes and mobility together shape cellular function and disease Cells organize their biochemical activities by assembling proteins into both membrane-bound organelles and membrane-less condensates. These compartments enable specialized chemical environments that s...

www.cell.com/trends/bioch...

The laser power was set to the max by the previous users!

COS7 cells?
I haven’t imaged them on either of those microscopes though!

a person is holding an empty wallet in their hands and it is empty . ALT: a person is holding an empty wallet in their hands and it is empty .

Damn!