U-box E3 structural mechanisms and immune signaling networks.
(a) NMR structure of the AtPUB14U-box (PDB: 1T1H, pale cyan).
(b) Superimposition of the AtPUB14U-box (PDB: 1T1H, pale cyan), GmPUB13U-box (PDB: 7C96, wheat), and OsCIE1U-box (PDB: 7XED, light pink).
(c) Crystal structure of the OsCIE1U-box (light pink) and OsUBC8 (light blue) complex (PDB: 7XED). (Bottom left) Close-up view of the E2-binding network with surface patch A. (Bottom middle) Close-up view of the OsCIE1U-box dimerization interface. (Bottom right) Close-up view of the E2-binding network with surface patch B.
(d) Crystal structure of the GmPUB13U-box (wheat)-Avr1d (pale green) complex (PDB: 7C96). (right) Zoom-in views of the interaction network between GmPUB13U-box-Avr1d.
(e) Two views of the crystal structure of the AvrPtoB CTD (C-terminal domain) (PDB: 2FD4, raspberry) showing the core fold, loops 1 and 2, and the partially disordered N-terminal domain regions.
(f) Surface and cartoon representation of the AvrPtoBCTD (PDB: 2FD4, raspberry). Close-up view of the E2-binding residues.
(g) Superimposition of the AtPUB14U-box (PDB: 1T1H, pale cyan) and AvrPtoBCTD (PDB: 2FD4, raspberry). Close-up view of the E2-binding residues.
(h) Models of U-box E3s in positively regulating immunity. (Left) PUB2/4-interacting PTI components in Arabidopsis. (Left middle) Chitin-activated CERK1 phosphorylation of CIE1 (i), preventing E3-ligase activity and promoting the immune response as well as PUB44 and the MAPK cascade (ii), promoting PBI1 degradation and releasing WRKY45, in rice. (Right middle) PUB17-mediated degradation of KH17 in potato. (Right) Positive feedback loop by the MYC2-PUB22-JAZ4 module to promote JA signaling in tomato.
(i) (Left) Secretion of SDE5 by CLas bacteria promotes PUB21-mediated MYC2 degradation and suppresses JA signaling in citrus. (Left middle) PUB25/26-mediated degradation of MYB6 and increased Verticillium dahliae susceptibility in Arabidopsis. (Right middle) PUB12/13-me…
🧬 From the plant journal: Ubiquitin ligases act as molecular switches that tune plant immunity, offering new routes to strengthen disease resistance without compromising growth. (Natalie Hamada, Katherine A. Hand, Nitzan Shabek)
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